Fractionating the Flavonoids in <i>Lonicerae japonicae</i> Flos and <i>Lonicerae flos</i> via Solvent Extraction Coupled with Automated Solid-Phase Extraction
Due to the structural diversity of flavonoids in functional plant foods and the inherent limitations of existing techniques, it is important to develop a simple and green (environmentally friendly) method of extracting flavonoids from plant foods. In this study, a method involving solvent extraction...
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MDPI AG
2024-11-01
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| Series: | Foods |
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| author | Lingyi Li Shanbo Zhang Bin Yu Shao Quan Liu Yancai Xiong |
| author_facet | Lingyi Li Shanbo Zhang Bin Yu Shao Quan Liu Yancai Xiong |
| author_sort | Lingyi Li |
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| description | Due to the structural diversity of flavonoids in functional plant foods and the inherent limitations of existing techniques, it is important to develop a simple and green (environmentally friendly) method of extracting flavonoids from plant foods. In this study, a method involving solvent extraction followed by automated solid-phase extraction was developed for extracting flavonoids from <i>Lonicerae japonicae</i> flos (JYH) and <i>Lonicerae flos</i> (SYH), both of which are widely used functional plant-based foods in Asian countries. For the optimisation of the solvent extraction method, solvent concentration (0.0, 20.0, 40.0, 60.0, 80.0 and 100.0% (<i>v</i>/<i>v</i>) of ethanol–water solution), extraction temperature (40, 60 and 80 °C) and extraction time (15.0, 30.0, 60.0, 90.0 and 120.0 min) were evaluated via design of experiment after screening. For solid-phase extraction, five cartridges (Strata-X, InertSep RP-2, InertSep RP-C18, Bond Elut-ENV, Oasis Prime HLB) were evaluated and different elution steps were optimised to obtain high recoveries (79.69–140.67%) for eight target flavonoids, including rutin, isoquercetin and luteolin. Antioxidant capacity assays revealed that JYH samples demonstrated superior antioxidant potential compared to SYH. The optimised extraction method provides a valuable tool for industrial-scale flavonoid production. |
| format | Article |
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| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2024-11-01 |
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| series | Foods |
| spelling | doaj-art-30d4e6b29ea44627841fce542b4d825e2024-12-13T16:26:50ZengMDPI AGFoods2304-81582024-11-011323386110.3390/foods13233861Fractionating the Flavonoids in <i>Lonicerae japonicae</i> Flos and <i>Lonicerae flos</i> via Solvent Extraction Coupled with Automated Solid-Phase ExtractionLingyi Li0Shanbo Zhang1Bin Yu2Shao Quan Liu3Yancai Xiong4Department of Food Science and Technology, National University of Singapore, S14 Level 5, Science Drive 2, Singapore 117542, SingaporeDepartment of Food Science and Technology, National University of Singapore, S14 Level 5, Science Drive 2, Singapore 117542, SingaporeMane SEA Pte Ltd., 3 Biopolis Drive, #07-17/18/19 Synapse, Singapore 138623, SingaporeDepartment of Food Science and Technology, National University of Singapore, S14 Level 5, Science Drive 2, Singapore 117542, SingaporeSchool of Pharmacy, Hubei University of Science and Technology, Xianning 437100, ChinaDue to the structural diversity of flavonoids in functional plant foods and the inherent limitations of existing techniques, it is important to develop a simple and green (environmentally friendly) method of extracting flavonoids from plant foods. In this study, a method involving solvent extraction followed by automated solid-phase extraction was developed for extracting flavonoids from <i>Lonicerae japonicae</i> flos (JYH) and <i>Lonicerae flos</i> (SYH), both of which are widely used functional plant-based foods in Asian countries. For the optimisation of the solvent extraction method, solvent concentration (0.0, 20.0, 40.0, 60.0, 80.0 and 100.0% (<i>v</i>/<i>v</i>) of ethanol–water solution), extraction temperature (40, 60 and 80 °C) and extraction time (15.0, 30.0, 60.0, 90.0 and 120.0 min) were evaluated via design of experiment after screening. For solid-phase extraction, five cartridges (Strata-X, InertSep RP-2, InertSep RP-C18, Bond Elut-ENV, Oasis Prime HLB) were evaluated and different elution steps were optimised to obtain high recoveries (79.69–140.67%) for eight target flavonoids, including rutin, isoquercetin and luteolin. Antioxidant capacity assays revealed that JYH samples demonstrated superior antioxidant potential compared to SYH. The optimised extraction method provides a valuable tool for industrial-scale flavonoid production.https://www.mdpi.com/2304-8158/13/23/3861<i>Lonicerae japonicae</i> flos<i>Lonicerae flos</i>flavonoidsgreen chemistryautomated solid-phase extractionantioxidant capacities |
| spellingShingle | Lingyi Li Shanbo Zhang Bin Yu Shao Quan Liu Yancai Xiong Fractionating the Flavonoids in <i>Lonicerae japonicae</i> Flos and <i>Lonicerae flos</i> via Solvent Extraction Coupled with Automated Solid-Phase Extraction Foods <i>Lonicerae japonicae</i> flos <i>Lonicerae flos</i> flavonoids green chemistry automated solid-phase extraction antioxidant capacities |
| title | Fractionating the Flavonoids in <i>Lonicerae japonicae</i> Flos and <i>Lonicerae flos</i> via Solvent Extraction Coupled with Automated Solid-Phase Extraction |
| title_full | Fractionating the Flavonoids in <i>Lonicerae japonicae</i> Flos and <i>Lonicerae flos</i> via Solvent Extraction Coupled with Automated Solid-Phase Extraction |
| title_fullStr | Fractionating the Flavonoids in <i>Lonicerae japonicae</i> Flos and <i>Lonicerae flos</i> via Solvent Extraction Coupled with Automated Solid-Phase Extraction |
| title_full_unstemmed | Fractionating the Flavonoids in <i>Lonicerae japonicae</i> Flos and <i>Lonicerae flos</i> via Solvent Extraction Coupled with Automated Solid-Phase Extraction |
| title_short | Fractionating the Flavonoids in <i>Lonicerae japonicae</i> Flos and <i>Lonicerae flos</i> via Solvent Extraction Coupled with Automated Solid-Phase Extraction |
| title_sort | fractionating the flavonoids in i lonicerae japonicae i flos and i lonicerae flos i via solvent extraction coupled with automated solid phase extraction |
| topic | <i>Lonicerae japonicae</i> flos <i>Lonicerae flos</i> flavonoids green chemistry automated solid-phase extraction antioxidant capacities |
| url | https://www.mdpi.com/2304-8158/13/23/3861 |
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