How Adhesion Molecule Patterns Change While Neutrophils Traffic through the Lung during Inflammation

In acute pulmonary inflammation, polymorphonuclear cells (PMNs) pass a transendothelial barrier from the circulation into the lung interstitium followed by a transepithelial migration into the alveolar space. These migration steps are regulated differentially by a concept of adhesion molecules and r...

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Main Authors: Franziska M. Konrad, Julia Wohlert, Jutta Gamper-Tsigaras, Kristian-Christos Ngamsri, Jörg Reutershan
Format: Article
Language:English
Published: Wiley 2019-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/2019/1208086
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author Franziska M. Konrad
Julia Wohlert
Jutta Gamper-Tsigaras
Kristian-Christos Ngamsri
Jörg Reutershan
author_facet Franziska M. Konrad
Julia Wohlert
Jutta Gamper-Tsigaras
Kristian-Christos Ngamsri
Jörg Reutershan
author_sort Franziska M. Konrad
collection DOAJ
description In acute pulmonary inflammation, polymorphonuclear cells (PMNs) pass a transendothelial barrier from the circulation into the lung interstitium followed by a transepithelial migration into the alveolar space. These migration steps are regulated differentially by a concept of adhesion molecules and remain—despite decades of research—incompletely understood. Current knowledge of changes in the expression pattern of adhesion molecules mainly derives from in vitro studies or from studies in extrapulmonary organ systems, where regulation of adhesion molecules differs significantly. In a murine model of lung inflammation, we determined the expression pattern of nine relevant neutrophilic adhesion molecules on their way through the different compartments of the lung. We used a flow cytometry-based technique that allowed describing spatial distribution of the adhesion molecules expressed on PMNs during their migration through the lung in detail. For example, the highest expression of CD29 was found in the intravascular compartment, highlighting its impact on the initial adhesion to the endothelium. CD47 showed its peak of expression on the later phase of transendothelial migration, whereas CD11b and CD54 expression peaked interstitial. A pivotal role for transepithelial migration was found for the adhesion molecule CD172a. Thereby, expression may correlate with functional impact for specific migration steps. In vitro studies further confirmed our in vivo findings. In conclusion, we are the first to determine the changes in expression patterns of relevant adhesion molecules on their migration through the different compartments of the lung. These findings may help to further understand the regulation of neutrophil trafficking in the lung.
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spelling doaj-art-30b9d889dc7544c986bdebb85d6f75252025-02-03T05:51:19ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/12080861208086How Adhesion Molecule Patterns Change While Neutrophils Traffic through the Lung during InflammationFranziska M. Konrad0Julia Wohlert1Jutta Gamper-Tsigaras2Kristian-Christos Ngamsri3Jörg Reutershan4Department of Anesthesiology and Intensive Care Medicine, University Hospital of Tübingen, Tübingen, GermanyDepartment of Anesthesiology and Intensive Care Medicine, University Hospital of Tübingen, Tübingen, GermanyDepartment of Anesthesiology and Intensive Care Medicine, University Hospital of Tübingen, Tübingen, GermanyDepartment of Anesthesiology and Intensive Care Medicine, University Hospital of Tübingen, Tübingen, GermanyDepartment of Anesthesiology and Intensive Care Medicine, Hospital of Bayreuth, GermanyIn acute pulmonary inflammation, polymorphonuclear cells (PMNs) pass a transendothelial barrier from the circulation into the lung interstitium followed by a transepithelial migration into the alveolar space. These migration steps are regulated differentially by a concept of adhesion molecules and remain—despite decades of research—incompletely understood. Current knowledge of changes in the expression pattern of adhesion molecules mainly derives from in vitro studies or from studies in extrapulmonary organ systems, where regulation of adhesion molecules differs significantly. In a murine model of lung inflammation, we determined the expression pattern of nine relevant neutrophilic adhesion molecules on their way through the different compartments of the lung. We used a flow cytometry-based technique that allowed describing spatial distribution of the adhesion molecules expressed on PMNs during their migration through the lung in detail. For example, the highest expression of CD29 was found in the intravascular compartment, highlighting its impact on the initial adhesion to the endothelium. CD47 showed its peak of expression on the later phase of transendothelial migration, whereas CD11b and CD54 expression peaked interstitial. A pivotal role for transepithelial migration was found for the adhesion molecule CD172a. Thereby, expression may correlate with functional impact for specific migration steps. In vitro studies further confirmed our in vivo findings. In conclusion, we are the first to determine the changes in expression patterns of relevant adhesion molecules on their migration through the different compartments of the lung. These findings may help to further understand the regulation of neutrophil trafficking in the lung.http://dx.doi.org/10.1155/2019/1208086
spellingShingle Franziska M. Konrad
Julia Wohlert
Jutta Gamper-Tsigaras
Kristian-Christos Ngamsri
Jörg Reutershan
How Adhesion Molecule Patterns Change While Neutrophils Traffic through the Lung during Inflammation
Mediators of Inflammation
title How Adhesion Molecule Patterns Change While Neutrophils Traffic through the Lung during Inflammation
title_full How Adhesion Molecule Patterns Change While Neutrophils Traffic through the Lung during Inflammation
title_fullStr How Adhesion Molecule Patterns Change While Neutrophils Traffic through the Lung during Inflammation
title_full_unstemmed How Adhesion Molecule Patterns Change While Neutrophils Traffic through the Lung during Inflammation
title_short How Adhesion Molecule Patterns Change While Neutrophils Traffic through the Lung during Inflammation
title_sort how adhesion molecule patterns change while neutrophils traffic through the lung during inflammation
url http://dx.doi.org/10.1155/2019/1208086
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