Evaluation of various filter paper and reagent systems for the preservation of Newcastle disease virus RNA samples

The transport of Newcastle disease virus (NDV) specimens, isolates or purified RNA is traditionally performed at ultra-low temperatures using dry ice to prevent degradation. However, this method is costly and requires specialised packaging and stringent shipping conditions. The aim of this study is...

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Main Authors: Qaisieh Bajes Amjed Al, Ababneh Mustafa Mohammed-Khair, Al-Zghoul Mohammad Borhan F., Alghizzawi Daoud Abed Alnaser, Aboomer Hebah Alaeddin
Format: Article
Language:English
Published: Sciendo 2025-06-01
Series:Journal of Veterinary Research
Subjects:
Online Access:https://doi.org/10.2478/jvetres-2025-0030
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author Qaisieh Bajes Amjed Al
Ababneh Mustafa Mohammed-Khair
Al-Zghoul Mohammad Borhan F.
Alghizzawi Daoud Abed Alnaser
Aboomer Hebah Alaeddin
author_facet Qaisieh Bajes Amjed Al
Ababneh Mustafa Mohammed-Khair
Al-Zghoul Mohammad Borhan F.
Alghizzawi Daoud Abed Alnaser
Aboomer Hebah Alaeddin
author_sort Qaisieh Bajes Amjed Al
collection DOAJ
description The transport of Newcastle disease virus (NDV) specimens, isolates or purified RNA is traditionally performed at ultra-low temperatures using dry ice to prevent degradation. However, this method is costly and requires specialised packaging and stringent shipping conditions. The aim of this study is to evaluate existing products’ capacities to preserve NDV or its RNA under different conditions.
format Article
id doaj-art-30b99be632914b099c5b4bc6838b4f4f
institution DOAJ
issn 2450-8608
language English
publishDate 2025-06-01
publisher Sciendo
record_format Article
series Journal of Veterinary Research
spelling doaj-art-30b99be632914b099c5b4bc6838b4f4f2025-08-20T03:15:10ZengSciendoJournal of Veterinary Research2450-86082025-06-0169218319010.2478/jvetres-2025-0030Evaluation of various filter paper and reagent systems for the preservation of Newcastle disease virus RNA samplesQaisieh Bajes Amjed Al0Ababneh Mustafa Mohammed-Khair1Al-Zghoul Mohammad Borhan F.2Alghizzawi Daoud Abed Alnaser3Aboomer Hebah Alaeddin41Department of Basic Medical Veterinary Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, 22110Irbid, Jordan1Department of Basic Medical Veterinary Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, 22110Irbid, Jordan1Department of Basic Medical Veterinary Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, 22110Irbid, Jordan1Department of Basic Medical Veterinary Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, 22110Irbid, Jordan2Department of Clinical Medical Veterinary Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, 22110Irbid, JordanThe transport of Newcastle disease virus (NDV) specimens, isolates or purified RNA is traditionally performed at ultra-low temperatures using dry ice to prevent degradation. However, this method is costly and requires specialised packaging and stringent shipping conditions. The aim of this study is to evaluate existing products’ capacities to preserve NDV or its RNA under different conditions.https://doi.org/10.2478/jvetres-2025-0030fta cardrna preservationnewcastle disease virusrna integrityambient temperature
spellingShingle Qaisieh Bajes Amjed Al
Ababneh Mustafa Mohammed-Khair
Al-Zghoul Mohammad Borhan F.
Alghizzawi Daoud Abed Alnaser
Aboomer Hebah Alaeddin
Evaluation of various filter paper and reagent systems for the preservation of Newcastle disease virus RNA samples
Journal of Veterinary Research
fta card
rna preservation
newcastle disease virus
rna integrity
ambient temperature
title Evaluation of various filter paper and reagent systems for the preservation of Newcastle disease virus RNA samples
title_full Evaluation of various filter paper and reagent systems for the preservation of Newcastle disease virus RNA samples
title_fullStr Evaluation of various filter paper and reagent systems for the preservation of Newcastle disease virus RNA samples
title_full_unstemmed Evaluation of various filter paper and reagent systems for the preservation of Newcastle disease virus RNA samples
title_short Evaluation of various filter paper and reagent systems for the preservation of Newcastle disease virus RNA samples
title_sort evaluation of various filter paper and reagent systems for the preservation of newcastle disease virus rna samples
topic fta card
rna preservation
newcastle disease virus
rna integrity
ambient temperature
url https://doi.org/10.2478/jvetres-2025-0030
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