Gene Expression Profiling in Behcet’s Disease Indicates an Autoimmune Component in the Pathogenesis of the Disease and Opens New Avenues for Targeted Therapy
Behçet disease (BD) is a chronic inflammatory multisystem disease characterized by oral and genital ulcers, uveitis, and skin lesions. Disease etiopathogenesis is still unclear. We aim to elucidate some aspects of BD pathogenesis and to identify specific gene signatures in peripheral blood cells (PB...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/4246965 |
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| author | Antonio Puccetti Piera Filomena Fiore Andrea Pelosi Elisa Tinazzi Giuseppe Patuzzo Giuseppe Argentino Francesca Moretta Claudio Lunardi Marzia Dolcino |
| author_facet | Antonio Puccetti Piera Filomena Fiore Andrea Pelosi Elisa Tinazzi Giuseppe Patuzzo Giuseppe Argentino Francesca Moretta Claudio Lunardi Marzia Dolcino |
| author_sort | Antonio Puccetti |
| collection | DOAJ |
| description | Behçet disease (BD) is a chronic inflammatory multisystem disease characterized by oral and genital ulcers, uveitis, and skin lesions. Disease etiopathogenesis is still unclear. We aim to elucidate some aspects of BD pathogenesis and to identify specific gene signatures in peripheral blood cells (PBCs) of patients with active disease using novel gene expression and network analysis. 179 genes were modulated in 10 PBCs of BD patients when compared to 10 healthy donors. Among differentially expressed genes the top enriched gene function was immune response, characterized by upregulation of Th17-related genes and type I interferon- (IFN-) inducible genes. Th17 polarization was confirmed by FACS analysis. The transcriptome identified gene classes (vascular damage, blood coagulation, and inflammation) involved in the pathogenesis of the typical features of BD. Following network analysis, the resulting interactome showed 5 highly connected regions (clusters) enriched in T and B cell activation pathways and 2 clusters enriched in type I IFN, JAK/STAT, and TLR signaling pathways, all implicated in autoimmune diseases. We report here the first combined analysis of the transcriptome and interactome in PBCs of BD patients in the active stage of disease. This approach generates useful insights in disease pathogenesis and suggests an autoimmune component in the origin of BD. |
| format | Article |
| id | doaj-art-30ad864c69ec4afda8cdcbedde7821ca |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-30ad864c69ec4afda8cdcbedde7821ca2025-08-20T02:07:55ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/42469654246965Gene Expression Profiling in Behcet’s Disease Indicates an Autoimmune Component in the Pathogenesis of the Disease and Opens New Avenues for Targeted TherapyAntonio Puccetti0Piera Filomena Fiore1Andrea Pelosi2Elisa Tinazzi3Giuseppe Patuzzo4Giuseppe Argentino5Francesca Moretta6Claudio Lunardi7Marzia Dolcino8Immunology Area, Pediatric Hospital Bambino Gesù, Viale San Paolo 15, 00146 Rome, ItalyImmunology Area, Pediatric Hospital Bambino Gesù, Viale San Paolo 15, 00146 Rome, ItalyImmunology Area, Pediatric Hospital Bambino Gesù, Viale San Paolo 15, 00146 Rome, ItalyDepartment of Medicine, University of Verona, Piazzale L.A. Scuro 10, 37134 Verona, ItalyDepartment of Medicine, University of Verona, Piazzale L.A. Scuro 10, 37134 Verona, ItalyDepartment of Medicine, University of Verona, Piazzale L.A. Scuro 10, 37134 Verona, ItalyDepartment of Medicine, University of Verona, Piazzale L.A. Scuro 10, 37134 Verona, ItalyDepartment of Medicine, University of Verona, Piazzale L.A. Scuro 10, 37134 Verona, ItalyDepartment of Medicine, University of Verona, Piazzale L.A. Scuro 10, 37134 Verona, ItalyBehçet disease (BD) is a chronic inflammatory multisystem disease characterized by oral and genital ulcers, uveitis, and skin lesions. Disease etiopathogenesis is still unclear. We aim to elucidate some aspects of BD pathogenesis and to identify specific gene signatures in peripheral blood cells (PBCs) of patients with active disease using novel gene expression and network analysis. 179 genes were modulated in 10 PBCs of BD patients when compared to 10 healthy donors. Among differentially expressed genes the top enriched gene function was immune response, characterized by upregulation of Th17-related genes and type I interferon- (IFN-) inducible genes. Th17 polarization was confirmed by FACS analysis. The transcriptome identified gene classes (vascular damage, blood coagulation, and inflammation) involved in the pathogenesis of the typical features of BD. Following network analysis, the resulting interactome showed 5 highly connected regions (clusters) enriched in T and B cell activation pathways and 2 clusters enriched in type I IFN, JAK/STAT, and TLR signaling pathways, all implicated in autoimmune diseases. We report here the first combined analysis of the transcriptome and interactome in PBCs of BD patients in the active stage of disease. This approach generates useful insights in disease pathogenesis and suggests an autoimmune component in the origin of BD.http://dx.doi.org/10.1155/2018/4246965 |
| spellingShingle | Antonio Puccetti Piera Filomena Fiore Andrea Pelosi Elisa Tinazzi Giuseppe Patuzzo Giuseppe Argentino Francesca Moretta Claudio Lunardi Marzia Dolcino Gene Expression Profiling in Behcet’s Disease Indicates an Autoimmune Component in the Pathogenesis of the Disease and Opens New Avenues for Targeted Therapy Journal of Immunology Research |
| title | Gene Expression Profiling in Behcet’s Disease Indicates an Autoimmune Component in the Pathogenesis of the Disease and Opens New Avenues for Targeted Therapy |
| title_full | Gene Expression Profiling in Behcet’s Disease Indicates an Autoimmune Component in the Pathogenesis of the Disease and Opens New Avenues for Targeted Therapy |
| title_fullStr | Gene Expression Profiling in Behcet’s Disease Indicates an Autoimmune Component in the Pathogenesis of the Disease and Opens New Avenues for Targeted Therapy |
| title_full_unstemmed | Gene Expression Profiling in Behcet’s Disease Indicates an Autoimmune Component in the Pathogenesis of the Disease and Opens New Avenues for Targeted Therapy |
| title_short | Gene Expression Profiling in Behcet’s Disease Indicates an Autoimmune Component in the Pathogenesis of the Disease and Opens New Avenues for Targeted Therapy |
| title_sort | gene expression profiling in behcet s disease indicates an autoimmune component in the pathogenesis of the disease and opens new avenues for targeted therapy |
| url | http://dx.doi.org/10.1155/2018/4246965 |
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