Diisooctyl phthalate (DIOP) exposure leads to cell apoptosis to HUVEC cells and toxicity to Caenorhabditis elegans through increasing the oxidative stress
Diisooctyl phthalate (DIOP), a common phthalate plasticizer, is frequently encountered in everyday life. Despite its widespread use, there is a dearth of toxicological research on DIOP, resulting in incomplete knowledge of its potential harmful effects. Our current research endeavored to provide a c...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324016701 |
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| author | Siyuan Luo Junnan Li Yuqing Zhou Zihang Zhai Qiang Li Zhenglin Huang Wencan He Kejun Zhong Bo Kong Zanxian Xia Hang Fai Kwok Lipeng Zhu |
| author_facet | Siyuan Luo Junnan Li Yuqing Zhou Zihang Zhai Qiang Li Zhenglin Huang Wencan He Kejun Zhong Bo Kong Zanxian Xia Hang Fai Kwok Lipeng Zhu |
| author_sort | Siyuan Luo |
| collection | DOAJ |
| description | Diisooctyl phthalate (DIOP), a common phthalate plasticizer, is frequently encountered in everyday life. Despite its widespread use, there is a dearth of toxicological research on DIOP, resulting in incomplete knowledge of its potential harmful effects. Our current research endeavored to provide a comprehensive evaluation of DIOP's toxicological profile using both cellular and Caenorhabditis elegans models as our in vitro and in vivo study subjects. Our results demonstrate that DIOP markedly decreases the viability and colony-forming ability of HUVECs. Moreover, this cytotoxicity correlates with elevated levels of reactive oxygen species (ROS), causing cell cycle arrest at the G1 phase and the induction of cell apoptosis. In addition, DIOP adversely affects the growth, movement, and reproductive fitness of C. elegans, as well as other physiological aspects such as body curvature, egg-laying capability, and body length. C. elegans exposed to DIOP exhibit increased oxidative stress, evidenced by higher ROS levels and lipofuscin buildup. Importantly, the PI3K/AKT and MAPK pathways are implicated in the response to DIOP-induced toxicity. This study, therefore, highlights the potential toxicity of DIOP in both cellular and organismal models, advancing our understanding of the detrimental effects associated with exposure to DIOP. |
| format | Article |
| id | doaj-art-30a4b1845fb145489c6b180309671d4f |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-30a4b1845fb145489c6b180309671d4f2025-02-12T05:29:55ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117594Diisooctyl phthalate (DIOP) exposure leads to cell apoptosis to HUVEC cells and toxicity to Caenorhabditis elegans through increasing the oxidative stressSiyuan Luo0Junnan Li1Yuqing Zhou2Zihang Zhai3Qiang Li4Zhenglin Huang5Wencan He6Kejun Zhong7Bo Kong8Zanxian Xia9Hang Fai Kwok10Lipeng Zhu11Department of Biomedical Sciences, Faculty of Health Sciences, University of Macau, University of Macau, 999078, MacaoKey Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, School of Medicine, Hunan Normal University, Changsha, Hunan, ChinaSchool of Life Sciences, Xiangya School of Medicine, Central South University, Changsha 510006, ChinaSchool of Life Sciences, Xiangya School of Medicine, Central South University, Changsha 510006, ChinaFood Safety Inspection Technology Center of Sichuan Market Supervision Administration, Chengdu, ChinaFood Safety Inspection Technology Center of Sichuan Market Supervision Administration, Chengdu, ChinaSchool of Life Sciences, Xiangya School of Medicine, Central South University, Changsha 510006, China; Technology Center, China Tobacco Hunan Industrial Co., Ltd., Changsha 410014, ChinaTechnology Center, China Tobacco Hunan Industrial Co., Ltd., Changsha 410014, ChinaTechnology Center, China Tobacco Hunan Industrial Co., Ltd., Changsha 410014, ChinaSchool of Life Sciences, Xiangya School of Medicine, Central South University, Changsha 510006, China; Corresponding authors.Department of Biomedical Sciences, Faculty of Health Sciences, University of Macau, University of Macau, 999078, Macao; Corresponding authors.School of Life Sciences, Xiangya School of Medicine, Central South University, Changsha 510006, China; Corresponding authors.Diisooctyl phthalate (DIOP), a common phthalate plasticizer, is frequently encountered in everyday life. Despite its widespread use, there is a dearth of toxicological research on DIOP, resulting in incomplete knowledge of its potential harmful effects. Our current research endeavored to provide a comprehensive evaluation of DIOP's toxicological profile using both cellular and Caenorhabditis elegans models as our in vitro and in vivo study subjects. Our results demonstrate that DIOP markedly decreases the viability and colony-forming ability of HUVECs. Moreover, this cytotoxicity correlates with elevated levels of reactive oxygen species (ROS), causing cell cycle arrest at the G1 phase and the induction of cell apoptosis. In addition, DIOP adversely affects the growth, movement, and reproductive fitness of C. elegans, as well as other physiological aspects such as body curvature, egg-laying capability, and body length. C. elegans exposed to DIOP exhibit increased oxidative stress, evidenced by higher ROS levels and lipofuscin buildup. Importantly, the PI3K/AKT and MAPK pathways are implicated in the response to DIOP-induced toxicity. This study, therefore, highlights the potential toxicity of DIOP in both cellular and organismal models, advancing our understanding of the detrimental effects associated with exposure to DIOP.http://www.sciencedirect.com/science/article/pii/S0147651324016701Diisooctyl phthalateToxicityCell apoptosisC. elegans |
| spellingShingle | Siyuan Luo Junnan Li Yuqing Zhou Zihang Zhai Qiang Li Zhenglin Huang Wencan He Kejun Zhong Bo Kong Zanxian Xia Hang Fai Kwok Lipeng Zhu Diisooctyl phthalate (DIOP) exposure leads to cell apoptosis to HUVEC cells and toxicity to Caenorhabditis elegans through increasing the oxidative stress Ecotoxicology and Environmental Safety Diisooctyl phthalate Toxicity Cell apoptosis C. elegans |
| title | Diisooctyl phthalate (DIOP) exposure leads to cell apoptosis to HUVEC cells and toxicity to Caenorhabditis elegans through increasing the oxidative stress |
| title_full | Diisooctyl phthalate (DIOP) exposure leads to cell apoptosis to HUVEC cells and toxicity to Caenorhabditis elegans through increasing the oxidative stress |
| title_fullStr | Diisooctyl phthalate (DIOP) exposure leads to cell apoptosis to HUVEC cells and toxicity to Caenorhabditis elegans through increasing the oxidative stress |
| title_full_unstemmed | Diisooctyl phthalate (DIOP) exposure leads to cell apoptosis to HUVEC cells and toxicity to Caenorhabditis elegans through increasing the oxidative stress |
| title_short | Diisooctyl phthalate (DIOP) exposure leads to cell apoptosis to HUVEC cells and toxicity to Caenorhabditis elegans through increasing the oxidative stress |
| title_sort | diisooctyl phthalate diop exposure leads to cell apoptosis to huvec cells and toxicity to caenorhabditis elegans through increasing the oxidative stress |
| topic | Diisooctyl phthalate Toxicity Cell apoptosis C. elegans |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324016701 |
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