Comparative RNA-Sequence Transcriptome Analysis of Phenolic Acid Metabolism in Salvia miltiorrhiza, a Traditional Chinese Medicine Model Plant
Salvia miltiorrhiza Bunge is an important traditional Chinese medicine (TCM). In this study, two S. miltiorrhiza genotypes (BH18 and ZH23) with different phenolic acid concentrations were used for de novo RNA sequencing (RNA-seq). A total of 170,787 transcripts and 56,216 unigenes were obtained. The...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2017/9364594 |
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| author | Zhenqiao Song Linlin Guo Tian Liu Caicai Lin Jianhua Wang Xingfeng Li |
| author_facet | Zhenqiao Song Linlin Guo Tian Liu Caicai Lin Jianhua Wang Xingfeng Li |
| author_sort | Zhenqiao Song |
| collection | DOAJ |
| description | Salvia miltiorrhiza Bunge is an important traditional Chinese medicine (TCM). In this study, two S. miltiorrhiza genotypes (BH18 and ZH23) with different phenolic acid concentrations were used for de novo RNA sequencing (RNA-seq). A total of 170,787 transcripts and 56,216 unigenes were obtained. There were 670 differentially expressed genes (DEGs) identified between BH18 and ZH23, 250 of which were upregulated in ZH23, with genes involved in the phenylpropanoid biosynthesis pathway being the most upregulated genes. Nine genes involved in the lignin biosynthesis pathway were upregulated in BH18 and thus result in higher lignin content in BH18. However, expression profiles of most genes involved in the core common upstream phenylpropanoid biosynthesis pathway were higher in ZH23 than that in BH18. These results indicated that genes involved in the core common upstream phenylpropanoid biosynthesis pathway might play an important role in downstream secondary metabolism and demonstrated that lignin biosynthesis was a putative partially competing pathway with phenolic acid biosynthesis. The results of this study expanded our understanding of the regulation of phenolic acid biosynthesis in S. miltiorrhiza. |
| format | Article |
| id | doaj-art-30a4a5f11586452eb1146da68d4e4673 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
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| series | International Journal of Genomics |
| spelling | doaj-art-30a4a5f11586452eb1146da68d4e46732025-08-20T03:55:12ZengWileyInternational Journal of Genomics2314-436X2314-43782017-01-01201710.1155/2017/93645949364594Comparative RNA-Sequence Transcriptome Analysis of Phenolic Acid Metabolism in Salvia miltiorrhiza, a Traditional Chinese Medicine Model PlantZhenqiao Song0Linlin Guo1Tian Liu2Caicai Lin3Jianhua Wang4Xingfeng Li5Agronomy College, Shandong Agricultural University, Tai’an, Shandong 271028, ChinaAgronomy College, Shandong Agricultural University, Tai’an, Shandong 271028, ChinaAgronomy College, Shandong Agricultural University, Tai’an, Shandong 271028, ChinaState Key Laboratory of Crop Biology, Agronomy College, Shandong Agricultural University, Tai’an, Shandong 271018, ChinaAgronomy College, Shandong Agricultural University, Tai’an, Shandong 271028, ChinaAgronomy College, Shandong Agricultural University, Tai’an, Shandong 271028, ChinaSalvia miltiorrhiza Bunge is an important traditional Chinese medicine (TCM). In this study, two S. miltiorrhiza genotypes (BH18 and ZH23) with different phenolic acid concentrations were used for de novo RNA sequencing (RNA-seq). A total of 170,787 transcripts and 56,216 unigenes were obtained. There were 670 differentially expressed genes (DEGs) identified between BH18 and ZH23, 250 of which were upregulated in ZH23, with genes involved in the phenylpropanoid biosynthesis pathway being the most upregulated genes. Nine genes involved in the lignin biosynthesis pathway were upregulated in BH18 and thus result in higher lignin content in BH18. However, expression profiles of most genes involved in the core common upstream phenylpropanoid biosynthesis pathway were higher in ZH23 than that in BH18. These results indicated that genes involved in the core common upstream phenylpropanoid biosynthesis pathway might play an important role in downstream secondary metabolism and demonstrated that lignin biosynthesis was a putative partially competing pathway with phenolic acid biosynthesis. The results of this study expanded our understanding of the regulation of phenolic acid biosynthesis in S. miltiorrhiza.http://dx.doi.org/10.1155/2017/9364594 |
| spellingShingle | Zhenqiao Song Linlin Guo Tian Liu Caicai Lin Jianhua Wang Xingfeng Li Comparative RNA-Sequence Transcriptome Analysis of Phenolic Acid Metabolism in Salvia miltiorrhiza, a Traditional Chinese Medicine Model Plant International Journal of Genomics |
| title | Comparative RNA-Sequence Transcriptome Analysis of Phenolic Acid Metabolism in Salvia miltiorrhiza, a Traditional Chinese Medicine Model Plant |
| title_full | Comparative RNA-Sequence Transcriptome Analysis of Phenolic Acid Metabolism in Salvia miltiorrhiza, a Traditional Chinese Medicine Model Plant |
| title_fullStr | Comparative RNA-Sequence Transcriptome Analysis of Phenolic Acid Metabolism in Salvia miltiorrhiza, a Traditional Chinese Medicine Model Plant |
| title_full_unstemmed | Comparative RNA-Sequence Transcriptome Analysis of Phenolic Acid Metabolism in Salvia miltiorrhiza, a Traditional Chinese Medicine Model Plant |
| title_short | Comparative RNA-Sequence Transcriptome Analysis of Phenolic Acid Metabolism in Salvia miltiorrhiza, a Traditional Chinese Medicine Model Plant |
| title_sort | comparative rna sequence transcriptome analysis of phenolic acid metabolism in salvia miltiorrhiza a traditional chinese medicine model plant |
| url | http://dx.doi.org/10.1155/2017/9364594 |
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