Molecular characterization of carbapenem-resistant and virulent plasmids in Klebsiella pneumoniae from patients with bloodstream infections in China
Bloodstream infections (BSIs) caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) are potentially life-threatening and an urgent threat to public health. The present study aims to clarify the characteristics of carbapenemase-encoding and virulent plasmids, and their interactions with the hos...
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Taylor & Francis Group
2021-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2021.1906163 |
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| author | Yongqiang Yang Yanxian Yang Guanping Chen Minmin Lin Yuan Chen Ruowen He Klibs N. Galvão Mohamed Abd El-Gawad El-Sayed Ahmed Adam P. Roberts Yiping Wu Lan-Lan Zhong Xiaoxue Liang Mingyang Qin Xin Ding Wenbin Deng Songyin Huang Hong-Yu Li Min Dai Ding-Qiang Chen Liyan Zhang Kang Liao Yong Xia Guo-Bao Tian |
| author_facet | Yongqiang Yang Yanxian Yang Guanping Chen Minmin Lin Yuan Chen Ruowen He Klibs N. Galvão Mohamed Abd El-Gawad El-Sayed Ahmed Adam P. Roberts Yiping Wu Lan-Lan Zhong Xiaoxue Liang Mingyang Qin Xin Ding Wenbin Deng Songyin Huang Hong-Yu Li Min Dai Ding-Qiang Chen Liyan Zhang Kang Liao Yong Xia Guo-Bao Tian |
| author_sort | Yongqiang Yang |
| collection | DOAJ |
| description | Bloodstream infections (BSIs) caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) are potentially life-threatening and an urgent threat to public health. The present study aims to clarify the characteristics of carbapenemase-encoding and virulent plasmids, and their interactions with the host bacterium. A total of 425 Kp isolates were collected from the blood of BSI patients from nine Chinese hospitals, between 2005 and 2019. Integrated epidemiological and genomic data showed that ST11 and ST307 Kp isolates were associated with nosocomial outbreak and transmission. Comparative analysis of 147 Kp genomes and 39 completely assembled chromosomes revealed extensive interruption of acrR by ISKpn26 in all Kp carbapenemase-2 (KPC-2)-producing ST11 Kp isolates, leading to activation of the AcrAB-Tolc multidrug efflux pump and a subsequent reduction in susceptibility to the last-resort antibiotic tigecycline and six other antibiotics. We described 29 KPC-2 plasmids showing diverse structures, two virulence plasmids in two KPC-2-producing Kp, and two novel multidrug-resistant (MDR)-virulent plasmids. This study revealed a multifactorial impact of KPC-2 plasmid on Kp, which may be associated with nosocomial dissemination of MDR isolates. |
| format | Article |
| id | doaj-art-30975a7293bb4ad4a33d4fbfcf3f13e3 |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-30975a7293bb4ad4a33d4fbfcf3f13e32025-08-20T02:24:43ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512021-01-0110170070910.1080/22221751.2021.1906163Molecular characterization of carbapenem-resistant and virulent plasmids in Klebsiella pneumoniae from patients with bloodstream infections in ChinaYongqiang Yang0Yanxian Yang1Guanping Chen2Minmin Lin3Yuan Chen4Ruowen He5Klibs N. Galvão6Mohamed Abd El-Gawad El-Sayed Ahmed7Adam P. Roberts8Yiping Wu9Lan-Lan Zhong10Xiaoxue Liang11Mingyang Qin12Xin Ding13Wenbin Deng14Songyin Huang15Hong-Yu Li16Min Dai17Ding-Qiang Chen18Liyan Zhang19Kang Liao20Yong Xia21Guo-Bao Tian22Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaDepartment of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaSun Yat-sen University School of Medicine, Guangzhou, People’s Republic of ChinaDepartment of Respiratory Medicine, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, People’s Republic of ChinaSun Yat-sen University School of Medicine, Guangzhou, People’s Republic of ChinaDepartment of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaDepartment of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, USADepartment of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaDepartment of Tropical Disease Biology, Liverpool School of Tropical Medicine, Pembroke Place, UKDepartment of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaDepartment of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaSchool of Laboratory Medicine, Chengdu Medical College, Chengdu, People’s Republic of ChinaBasic Medical College, Xinxiang Medical University, Xinxiang, People’s Republic of ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Guangzhou, People’s Republic of ChinaSchool of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Guangzhou, People’s Republic of ChinaDepartment of Clinical Laboratory, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaDepartment of Clinical Laboratory, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaSchool of Laboratory Medicine, Chengdu Medical College, Chengdu, People’s Republic of ChinaDivision of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, People’s Republic of ChinaDepartment of Clinical Laboratory, Guangdong Provincial People’s Hospital / Guangdong Academy of Medical Sciences, Guangzhou, People’s Republic of ChinaDepartment of Clinical Laboratory, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, People’s Republic of ChinaDepartment of Clinical Laboratory Medicine, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, People’s Republic of ChinaDepartment of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, People’s Republic of ChinaBloodstream infections (BSIs) caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) are potentially life-threatening and an urgent threat to public health. The present study aims to clarify the characteristics of carbapenemase-encoding and virulent plasmids, and their interactions with the host bacterium. A total of 425 Kp isolates were collected from the blood of BSI patients from nine Chinese hospitals, between 2005 and 2019. Integrated epidemiological and genomic data showed that ST11 and ST307 Kp isolates were associated with nosocomial outbreak and transmission. Comparative analysis of 147 Kp genomes and 39 completely assembled chromosomes revealed extensive interruption of acrR by ISKpn26 in all Kp carbapenemase-2 (KPC-2)-producing ST11 Kp isolates, leading to activation of the AcrAB-Tolc multidrug efflux pump and a subsequent reduction in susceptibility to the last-resort antibiotic tigecycline and six other antibiotics. We described 29 KPC-2 plasmids showing diverse structures, two virulence plasmids in two KPC-2-producing Kp, and two novel multidrug-resistant (MDR)-virulent plasmids. This study revealed a multifactorial impact of KPC-2 plasmid on Kp, which may be associated with nosocomial dissemination of MDR isolates.https://www.tandfonline.com/doi/10.1080/22221751.2021.1906163Bloodstream infectioncarbapenem resistanceKlebsiella pneumoniaegenomicsKPC-2 |
| spellingShingle | Yongqiang Yang Yanxian Yang Guanping Chen Minmin Lin Yuan Chen Ruowen He Klibs N. Galvão Mohamed Abd El-Gawad El-Sayed Ahmed Adam P. Roberts Yiping Wu Lan-Lan Zhong Xiaoxue Liang Mingyang Qin Xin Ding Wenbin Deng Songyin Huang Hong-Yu Li Min Dai Ding-Qiang Chen Liyan Zhang Kang Liao Yong Xia Guo-Bao Tian Molecular characterization of carbapenem-resistant and virulent plasmids in Klebsiella pneumoniae from patients with bloodstream infections in China Emerging Microbes and Infections Bloodstream infection carbapenem resistance Klebsiella pneumoniae genomics KPC-2 |
| title | Molecular characterization of carbapenem-resistant and virulent plasmids in Klebsiella pneumoniae from patients with bloodstream infections in China |
| title_full | Molecular characterization of carbapenem-resistant and virulent plasmids in Klebsiella pneumoniae from patients with bloodstream infections in China |
| title_fullStr | Molecular characterization of carbapenem-resistant and virulent plasmids in Klebsiella pneumoniae from patients with bloodstream infections in China |
| title_full_unstemmed | Molecular characterization of carbapenem-resistant and virulent plasmids in Klebsiella pneumoniae from patients with bloodstream infections in China |
| title_short | Molecular characterization of carbapenem-resistant and virulent plasmids in Klebsiella pneumoniae from patients with bloodstream infections in China |
| title_sort | molecular characterization of carbapenem resistant and virulent plasmids in klebsiella pneumoniae from patients with bloodstream infections in china |
| topic | Bloodstream infection carbapenem resistance Klebsiella pneumoniae genomics KPC-2 |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2021.1906163 |
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