The green algae CO2 concentrating mechanism and photorespiration jointly operate during acclimation to low CO2
Abstract Due to low availability of CO2 in aquatic environment, microalgae have evolved a CO2 concentrating mechanism (CCM). It has long been thought that operation of CCM would suppress photorespiration by increasing the CO2 concentration at the Rubisco active site, but experimental evidence is sca...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-06-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-60525-7 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850111740823470080 |
|---|---|
| author | Ousmane Dao Marie Bertrand Saleh Alseekh Florian Veillet Pascaline Auroy Phuong-Chi Nguyen Bertrand Légeret Virginie Epting Amélie Morin Stephan Cuiné Caroline L. Monteil Luke C. M. Mackinder Adrien Burlacot Anja Krieger-Liszkay Andreas P. M. Weber Alisdair R. Fernie Gilles Peltier Yonghua Li-Beisson |
| author_facet | Ousmane Dao Marie Bertrand Saleh Alseekh Florian Veillet Pascaline Auroy Phuong-Chi Nguyen Bertrand Légeret Virginie Epting Amélie Morin Stephan Cuiné Caroline L. Monteil Luke C. M. Mackinder Adrien Burlacot Anja Krieger-Liszkay Andreas P. M. Weber Alisdair R. Fernie Gilles Peltier Yonghua Li-Beisson |
| author_sort | Ousmane Dao |
| collection | DOAJ |
| description | Abstract Due to low availability of CO2 in aquatic environment, microalgae have evolved a CO2 concentrating mechanism (CCM). It has long been thought that operation of CCM would suppress photorespiration by increasing the CO2 concentration at the Rubisco active site, but experimental evidence is scarce. To better explore the function of photorespiration in algae, we first characterized a Chlamydomonas reinhardtii mutant defected in low-CO2 inducible 20 (LCI20) and show that LCI20 is a chloroplast-envelope glutamate/malate transporter playing a role in photorespiration. By monitoring growth and glycolate excretion in mutants deficient in either CCM or photorespiration, we conclude that: (i.) CCM induction does not depend on photorespiration, (ii.) glycolate excretion together with glycolate dehydrogenase down-regulation prevents the toxic accumulation of non-metabolized photorespiratory metabolites, and (iii.) photorespiration is active at low CO2 when the CCM is operational. This work provides a foundation for a better understanding of the carbon cycle in the ocean where significant glycolate concentrations have been found. |
| format | Article |
| id | doaj-art-309121485a7e4bedb0ff5d836d08f183 |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-309121485a7e4bedb0ff5d836d08f1832025-08-20T02:37:33ZengNature PortfolioNature Communications2041-17232025-06-0116111210.1038/s41467-025-60525-7The green algae CO2 concentrating mechanism and photorespiration jointly operate during acclimation to low CO2Ousmane Dao0Marie Bertrand1Saleh Alseekh2Florian Veillet3Pascaline Auroy4Phuong-Chi Nguyen5Bertrand Légeret6Virginie Epting7Amélie Morin8Stephan Cuiné9Caroline L. Monteil10Luke C. M. Mackinder11Adrien Burlacot12Anja Krieger-Liszkay13Andreas P. M. Weber14Alisdair R. Fernie15Gilles Peltier16Yonghua Li-Beisson17Aix-Marseille Université, CEA, CNRS, BIAM, UMR7265, Institut de Biosciences et Biotechnologies Aix-Marseille, CEA CadaracheAix-Marseille Université, CEA, CNRS, BIAM, UMR7265, Institut de Biosciences et Biotechnologies Aix-Marseille, CEA CadaracheDepartment of Molecular Physiology, Max Planck Institute of Molecular Plant PhysiologyAix-Marseille Université, CEA, CNRS, BIAM, UMR7265, Institut de Biosciences et Biotechnologies Aix-Marseille, CEA CadaracheAix-Marseille Université, CEA, CNRS, BIAM, UMR7265, Institut de Biosciences et Biotechnologies Aix-Marseille, CEA CadaracheAix-Marseille Université, CEA, CNRS, BIAM, UMR7265, Institut de Biosciences et Biotechnologies Aix-Marseille, CEA CadaracheAix-Marseille Université, CEA, CNRS, BIAM, UMR7265, Institut de Biosciences et Biotechnologies Aix-Marseille, CEA CadaracheAix-Marseille Université, CEA, CNRS, BIAM, UMR7265, Institut de Biosciences et Biotechnologies Aix-Marseille, CEA CadaracheAix-Marseille Université, CEA, CNRS, BIAM, UMR7265, Institut de Biosciences et Biotechnologies Aix-Marseille, CEA CadaracheAix-Marseille Université, CEA, CNRS, BIAM, UMR7265, Institut de Biosciences et Biotechnologies Aix-Marseille, CEA CadaracheAix-Marseille Université, CEA, CNRS, BIAM, UMR7265, Institut de Biosciences et Biotechnologies Aix-Marseille, CEA CadaracheCentre for Novel Agricultural Products, Department of Biology, University of YorkDepartment of Plant Biology, Division of Biosphere Sciences and Engineering, The Carnegie Institution for ScienceUniversité Paris-Saclay, Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, CEDEXInstitute of Plant Biochemistry, Cluster of Excellence on Plant Science (CEPLAS), Heinrich Heine UniversityDepartment of Molecular Physiology, Max Planck Institute of Molecular Plant PhysiologyAix-Marseille Université, CEA, CNRS, BIAM, UMR7265, Institut de Biosciences et Biotechnologies Aix-Marseille, CEA CadaracheAix-Marseille Université, CEA, CNRS, BIAM, UMR7265, Institut de Biosciences et Biotechnologies Aix-Marseille, CEA CadaracheAbstract Due to low availability of CO2 in aquatic environment, microalgae have evolved a CO2 concentrating mechanism (CCM). It has long been thought that operation of CCM would suppress photorespiration by increasing the CO2 concentration at the Rubisco active site, but experimental evidence is scarce. To better explore the function of photorespiration in algae, we first characterized a Chlamydomonas reinhardtii mutant defected in low-CO2 inducible 20 (LCI20) and show that LCI20 is a chloroplast-envelope glutamate/malate transporter playing a role in photorespiration. By monitoring growth and glycolate excretion in mutants deficient in either CCM or photorespiration, we conclude that: (i.) CCM induction does not depend on photorespiration, (ii.) glycolate excretion together with glycolate dehydrogenase down-regulation prevents the toxic accumulation of non-metabolized photorespiratory metabolites, and (iii.) photorespiration is active at low CO2 when the CCM is operational. This work provides a foundation for a better understanding of the carbon cycle in the ocean where significant glycolate concentrations have been found.https://doi.org/10.1038/s41467-025-60525-7 |
| spellingShingle | Ousmane Dao Marie Bertrand Saleh Alseekh Florian Veillet Pascaline Auroy Phuong-Chi Nguyen Bertrand Légeret Virginie Epting Amélie Morin Stephan Cuiné Caroline L. Monteil Luke C. M. Mackinder Adrien Burlacot Anja Krieger-Liszkay Andreas P. M. Weber Alisdair R. Fernie Gilles Peltier Yonghua Li-Beisson The green algae CO2 concentrating mechanism and photorespiration jointly operate during acclimation to low CO2 Nature Communications |
| title | The green algae CO2 concentrating mechanism and photorespiration jointly operate during acclimation to low CO2 |
| title_full | The green algae CO2 concentrating mechanism and photorespiration jointly operate during acclimation to low CO2 |
| title_fullStr | The green algae CO2 concentrating mechanism and photorespiration jointly operate during acclimation to low CO2 |
| title_full_unstemmed | The green algae CO2 concentrating mechanism and photorespiration jointly operate during acclimation to low CO2 |
| title_short | The green algae CO2 concentrating mechanism and photorespiration jointly operate during acclimation to low CO2 |
| title_sort | green algae co2 concentrating mechanism and photorespiration jointly operate during acclimation to low co2 |
| url | https://doi.org/10.1038/s41467-025-60525-7 |
| work_keys_str_mv | AT ousmanedao thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT mariebertrand thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT salehalseekh thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT florianveillet thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT pascalineauroy thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT phuongchinguyen thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT bertrandlegeret thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT virginieepting thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT ameliemorin thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT stephancuine thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT carolinelmonteil thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT lukecmmackinder thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT adrienburlacot thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT anjakriegerliszkay thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT andreaspmweber thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT alisdairrfernie thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT gillespeltier thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT yonghualibeisson thegreenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT ousmanedao greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT mariebertrand greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT salehalseekh greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT florianveillet greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT pascalineauroy greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT phuongchinguyen greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT bertrandlegeret greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT virginieepting greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT ameliemorin greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT stephancuine greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT carolinelmonteil greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT lukecmmackinder greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT adrienburlacot greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT anjakriegerliszkay greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT andreaspmweber greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT alisdairrfernie greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT gillespeltier greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 AT yonghualibeisson greenalgaeco2concentratingmechanismandphotorespirationjointlyoperateduringacclimationtolowco2 |