Selective Gene Transfer to the Retina Using Intravitreal Ultrasound Irradiation
This paper aims to evaluate the efficacy of intravitreal ultrasound (US) irradiation for green fluorescent protein (GFP) plasmid transfer into the rabbit retina using a miniature US transducer. Intravitreal US irradiation was performed by a slight modification of the transconjunctival sutureless vit...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2012/412752 |
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| author | Shozo Sonoda Katsuro Tachibana Toshifumi Yamashita Makoto Shirasawa Hiroto Terasaki Eisuke Uchino Ryo Suzuki Kazuo Maruyama Taiji Sakamoto |
| author_facet | Shozo Sonoda Katsuro Tachibana Toshifumi Yamashita Makoto Shirasawa Hiroto Terasaki Eisuke Uchino Ryo Suzuki Kazuo Maruyama Taiji Sakamoto |
| author_sort | Shozo Sonoda |
| collection | DOAJ |
| description | This paper aims to evaluate the efficacy of intravitreal ultrasound (US) irradiation for green fluorescent protein (GFP) plasmid transfer into the rabbit retina using a miniature US transducer. Intravitreal US irradiation was performed by a slight modification of the transconjunctival sutureless vitrectomy system utilizing a small probe. After vitrectomy, the US probe was inserted through a scleral incision. A mixture of GFP plasmid (50 μL) and bubble liposomes (BLs; 50 μL) was injected into the vitreous cavity, and US was generated to the retina using a SonoPore 4000. The control group was not exposed to US. After 72 h, the gene-transfer efficiency was quantified by counting the number of GFP-positive cells. The retinas that received plasmid, BL, and US showed a significant increase in the number (average ± SEM) of GFP-positive cells (32±4.9; n=7; P<0.01 ). No GFP-positive cells were observed in the control eyes (n=7). Intravitreal retinal US irradiation can transfer the GFP plasmid into the retina without causing any apparent damage. This procedure could be used to transfer genes and drugs directly to the retina and therefore has potential therapeutic value. |
| format | Article |
| id | doaj-art-30837146abc749ba890de0ac7ccc3dff |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-30837146abc749ba890de0ac7ccc3dff2025-02-03T06:08:17ZengWileyJournal of Ophthalmology2090-004X2090-00582012-01-01201210.1155/2012/412752412752Selective Gene Transfer to the Retina Using Intravitreal Ultrasound IrradiationShozo Sonoda0Katsuro Tachibana1Toshifumi Yamashita2Makoto Shirasawa3Hiroto Terasaki4Eisuke Uchino5Ryo Suzuki6Kazuo Maruyama7Taiji Sakamoto8Department of Ophthalmology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8520, JapanDepartment of Anatomy, School of Medicine, Fukuoka University, Fukuoka 814-0180, JapanDepartment of Ophthalmology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8520, JapanDepartment of Ophthalmology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8520, JapanDepartment of Ophthalmology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8520, JapanDepartment of Ophthalmology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8520, JapanDepartment of Biopharmaceutics, School of Pharmaceutical Sciences, Teikyo University, Sagamihara 229-0195, JapanDepartment of Biopharmaceutics, School of Pharmaceutical Sciences, Teikyo University, Sagamihara 229-0195, JapanDepartment of Ophthalmology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8520, JapanThis paper aims to evaluate the efficacy of intravitreal ultrasound (US) irradiation for green fluorescent protein (GFP) plasmid transfer into the rabbit retina using a miniature US transducer. Intravitreal US irradiation was performed by a slight modification of the transconjunctival sutureless vitrectomy system utilizing a small probe. After vitrectomy, the US probe was inserted through a scleral incision. A mixture of GFP plasmid (50 μL) and bubble liposomes (BLs; 50 μL) was injected into the vitreous cavity, and US was generated to the retina using a SonoPore 4000. The control group was not exposed to US. After 72 h, the gene-transfer efficiency was quantified by counting the number of GFP-positive cells. The retinas that received plasmid, BL, and US showed a significant increase in the number (average ± SEM) of GFP-positive cells (32±4.9; n=7; P<0.01 ). No GFP-positive cells were observed in the control eyes (n=7). Intravitreal retinal US irradiation can transfer the GFP plasmid into the retina without causing any apparent damage. This procedure could be used to transfer genes and drugs directly to the retina and therefore has potential therapeutic value.http://dx.doi.org/10.1155/2012/412752 |
| spellingShingle | Shozo Sonoda Katsuro Tachibana Toshifumi Yamashita Makoto Shirasawa Hiroto Terasaki Eisuke Uchino Ryo Suzuki Kazuo Maruyama Taiji Sakamoto Selective Gene Transfer to the Retina Using Intravitreal Ultrasound Irradiation Journal of Ophthalmology |
| title | Selective Gene Transfer to the Retina Using Intravitreal Ultrasound Irradiation |
| title_full | Selective Gene Transfer to the Retina Using Intravitreal Ultrasound Irradiation |
| title_fullStr | Selective Gene Transfer to the Retina Using Intravitreal Ultrasound Irradiation |
| title_full_unstemmed | Selective Gene Transfer to the Retina Using Intravitreal Ultrasound Irradiation |
| title_short | Selective Gene Transfer to the Retina Using Intravitreal Ultrasound Irradiation |
| title_sort | selective gene transfer to the retina using intravitreal ultrasound irradiation |
| url | http://dx.doi.org/10.1155/2012/412752 |
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