Transcriptomic Analysis of Pathogenicity Genes in Sclerotinia sclerotiorum Affecting Brassica napus
Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum is one of the most destructive diseases of rapeseed (Brassica napus L.) globally. This study identifies key genes involved in the pathogenicity of S. sclerotiorum during rapeseed infection. It examines these genes' codon usage bias (...
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University of Mazandaran
2024-09-01
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| Series: | Journal of Genetic Resources |
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| Online Access: | https://sc.journals.umz.ac.ir/article_5283_2d64d1dba93a661dcb2eaa07d43a4c79.pdf |
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| author | Hengameh Taheri Mohamad Hamed Ghodoum Parizipour |
| author_facet | Hengameh Taheri Mohamad Hamed Ghodoum Parizipour |
| author_sort | Hengameh Taheri |
| collection | DOAJ |
| description | Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum is one of the most destructive diseases of rapeseed (Brassica napus L.) globally. This study identifies key genes involved in the pathogenicity of S. sclerotiorum during rapeseed infection. It examines these genes' codon usage bias (CUB) to find the optimal codons effective in gene expression and the factors effective in the formation of CUB. Protein-protein interaction network was drawn and several hub genes were determined and ranked according to the CytoHubba tool among which the genes encoding nitrate reductase, beta-glucosidase, glucanase, PA14 domain-containing protein, carbohydrate-binding module family 1, and acyl-coenzyme A oxidase were found to be associated with the SSR induction. Gene ontology (GO) analysis showed that the genes related to the metabolism of organic substances, catalytic activity, and cellular anatomical entity had the highest count. Also, KEGG pathways analysis revealed 16 biological pathways modulated by S. sclerotiorum among which the genes associated with the metabolic pathways exhibited the highest count. CUB indices including CAI (codon adaptation index), ENC (effective number of codons), GC, GC3S (GC content in the third open position of the codon), and RSCU (relative synonymous codon usage) were determined and the results showed a significant positive correlation between GC and GC3S. Also, the possible effects of mutation pressure and natural selection in shaping CUB were determined. The mean ENC range from 42.4-56.65 indicating less orientation in codon usage. The range CAI was found to be 0.74-0.89 indicating the importance of genes in adapting to environmental stresses. The maximum RSCU was 1.76 for the CCA codon, which encodes the amino acid (aa) proline with a high preference for that codon compared to other synonymous codons of that aa. These results demonstrated that S. sclerotiorum modulates some genes involved in disease induction (e.g., cell wall-degrading enzymes, biosynthesis of secondary metabolites & metabolic pathways) to infect its host plant. |
| format | Article |
| id | doaj-art-30612f03b5f14f6bb33712cf2e9e568b |
| institution | Kabale University |
| issn | 2423-4257 2588-2589 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | University of Mazandaran |
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| series | Journal of Genetic Resources |
| spelling | doaj-art-30612f03b5f14f6bb33712cf2e9e568b2025-01-27T06:11:47ZengUniversity of MazandaranJournal of Genetic Resources2423-42572588-25892024-09-0110223224310.22080/jgr.2025.27934.14055283Transcriptomic Analysis of Pathogenicity Genes in Sclerotinia sclerotiorum Affecting Brassica napusHengameh Taheri0Mohamad Hamed Ghodoum Parizipour1Department of Plant Production and Genetics, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, IranDepartment of Plant Protection, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran Mollasani, Iran.Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum is one of the most destructive diseases of rapeseed (Brassica napus L.) globally. This study identifies key genes involved in the pathogenicity of S. sclerotiorum during rapeseed infection. It examines these genes' codon usage bias (CUB) to find the optimal codons effective in gene expression and the factors effective in the formation of CUB. Protein-protein interaction network was drawn and several hub genes were determined and ranked according to the CytoHubba tool among which the genes encoding nitrate reductase, beta-glucosidase, glucanase, PA14 domain-containing protein, carbohydrate-binding module family 1, and acyl-coenzyme A oxidase were found to be associated with the SSR induction. Gene ontology (GO) analysis showed that the genes related to the metabolism of organic substances, catalytic activity, and cellular anatomical entity had the highest count. Also, KEGG pathways analysis revealed 16 biological pathways modulated by S. sclerotiorum among which the genes associated with the metabolic pathways exhibited the highest count. CUB indices including CAI (codon adaptation index), ENC (effective number of codons), GC, GC3S (GC content in the third open position of the codon), and RSCU (relative synonymous codon usage) were determined and the results showed a significant positive correlation between GC and GC3S. Also, the possible effects of mutation pressure and natural selection in shaping CUB were determined. The mean ENC range from 42.4-56.65 indicating less orientation in codon usage. The range CAI was found to be 0.74-0.89 indicating the importance of genes in adapting to environmental stresses. The maximum RSCU was 1.76 for the CCA codon, which encodes the amino acid (aa) proline with a high preference for that codon compared to other synonymous codons of that aa. These results demonstrated that S. sclerotiorum modulates some genes involved in disease induction (e.g., cell wall-degrading enzymes, biosynthesis of secondary metabolites & metabolic pathways) to infect its host plant.https://sc.journals.umz.ac.ir/article_5283_2d64d1dba93a661dcb2eaa07d43a4c79.pdfgene ontologygene expressionpathogenicityplant-pathogen interactionsclerotinia sclerotiorumtranscriptomics |
| spellingShingle | Hengameh Taheri Mohamad Hamed Ghodoum Parizipour Transcriptomic Analysis of Pathogenicity Genes in Sclerotinia sclerotiorum Affecting Brassica napus Journal of Genetic Resources gene ontology gene expression pathogenicity plant-pathogen interaction sclerotinia sclerotiorum transcriptomics |
| title | Transcriptomic Analysis of Pathogenicity Genes in Sclerotinia sclerotiorum Affecting Brassica napus |
| title_full | Transcriptomic Analysis of Pathogenicity Genes in Sclerotinia sclerotiorum Affecting Brassica napus |
| title_fullStr | Transcriptomic Analysis of Pathogenicity Genes in Sclerotinia sclerotiorum Affecting Brassica napus |
| title_full_unstemmed | Transcriptomic Analysis of Pathogenicity Genes in Sclerotinia sclerotiorum Affecting Brassica napus |
| title_short | Transcriptomic Analysis of Pathogenicity Genes in Sclerotinia sclerotiorum Affecting Brassica napus |
| title_sort | transcriptomic analysis of pathogenicity genes in sclerotinia sclerotiorum affecting brassica napus |
| topic | gene ontology gene expression pathogenicity plant-pathogen interaction sclerotinia sclerotiorum transcriptomics |
| url | https://sc.journals.umz.ac.ir/article_5283_2d64d1dba93a661dcb2eaa07d43a4c79.pdf |
| work_keys_str_mv | AT hengamehtaheri transcriptomicanalysisofpathogenicitygenesinsclerotiniasclerotiorumaffectingbrassicanapus AT mohamadhamedghodoumparizipour transcriptomicanalysisofpathogenicitygenesinsclerotiniasclerotiorumaffectingbrassicanapus |