Effects of dietary ε-polylysine supplementation on growth performance, antioxidant capacity, immune function, and intestinal microbiota in growing male minks
IntroductionThis study investigated the effects of dietary ε-polylysine (ε-PL) supplementation on the growth performance, antioxidant capacity, immune function and intestinal microbiota in growing male minks.MethodsNinety-six 12-week-old male minks were randomly divided into 6 treatments (8 replicat...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1569620/full |
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| author | Nianxue Wang Heliang Wang Xiaowen Zhang Lianwen Zhao Wenli Li Beibei Zhang |
| author_facet | Nianxue Wang Heliang Wang Xiaowen Zhang Lianwen Zhao Wenli Li Beibei Zhang |
| author_sort | Nianxue Wang |
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| description | IntroductionThis study investigated the effects of dietary ε-polylysine (ε-PL) supplementation on the growth performance, antioxidant capacity, immune function and intestinal microbiota in growing male minks.MethodsNinety-six 12-week-old male minks were randomly divided into 6 treatments (8 replicates per treatment and 2 minks per replicate). Minks were fed basal diets supplemented with 0 (control), 100, 200, 300, 400, or 500 mg/kg ε-PL for 8 weeks.ResultsCompared with the control, 300–500 mg/kg ε-PL significantly increased the average daily gain (p < 0.05), and significantly decreased the feed-to-gain ratio (p < 0.05) during the whole period and significantly enhanced the body weight at week 8 (p < 0.05), 300–400 mg/kg ε-PL significantly increased the fresh pelt weight (p < 0.05). Compared with the control, 300 mg/kg ε-PL significantly increased serum T-SOD activity and jejunal mucosal T-SOD and GSH-Px activities (p < 0.05). Compared to the control, 200–400 mg/kg ε-PL significantly increased serum IgA level (p < 0.05), 300–400 mg/kg ε-PL significantly increased serum IgM level (p < 0.05), 400–500 mg/kg ε-PL significantly increased serum IgG level (p < 0.05). Compared with the control, 200–400 mg/kg ε-PL significantly increased jejunal mucosal IgA level (p < 0.05), 100 mg/kg ε-PL significantly increased jejunal mucosal IgM level (p < 0.05), 100–400 mg/kg ε-PL significantly increased jejunal mucosal IgG level (p < 0.05). Compared with the control group, all ε-PL supplemented groups significantly decreased serum IL-2 and IL-8 levels compared to the control (p < 0.05). The 16S rRNA sequencing analysis revealed that compared to the control, 300 mg/kg ε-PL significantly increased the relative abundance of Firmicutes and Clostridium_sensu_stricto_1 (p < 0.05), and significantly decreased the relative abundance of Proteobacteria and Escherichia-Shigella in ileal mucosa (p < 0.05). Spearman correlation analysis indicated that the relative abundance of Escherichia-Shigella was negatively correlated with the growth performance. The relative abundance of Clostridium_sensu_stricto_1 was positively correlated with the jejunal mucosal antioxidant indicators and immunoglobulin levels.DiscussionIn conclusion, dietary ε-PL supplementation can improve growth performance, antioxidant capacity, immune function, and gut microbial community in growing male minks, and the optimal dosage of ε-PL is 300 mg/kg. |
| format | Article |
| id | doaj-art-3033038a5bc44ecf94a23cfd5f468c0f |
| institution | DOAJ |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
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| spelling | doaj-art-3033038a5bc44ecf94a23cfd5f468c0f2025-08-20T02:57:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-05-011610.3389/fmicb.2025.15696201569620Effects of dietary ε-polylysine supplementation on growth performance, antioxidant capacity, immune function, and intestinal microbiota in growing male minksNianxue Wang0Heliang Wang1Xiaowen Zhang2Lianwen Zhao3Wenli Li4Beibei Zhang5College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, ChinaAnimal Health Development Research Center, Qingdao Qiushi College, Qingdao, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao, ChinaCollege of Animal Science and Technology, Qingdao Agricultural University, Qingdao, ChinaIntroductionThis study investigated the effects of dietary ε-polylysine (ε-PL) supplementation on the growth performance, antioxidant capacity, immune function and intestinal microbiota in growing male minks.MethodsNinety-six 12-week-old male minks were randomly divided into 6 treatments (8 replicates per treatment and 2 minks per replicate). Minks were fed basal diets supplemented with 0 (control), 100, 200, 300, 400, or 500 mg/kg ε-PL for 8 weeks.ResultsCompared with the control, 300–500 mg/kg ε-PL significantly increased the average daily gain (p < 0.05), and significantly decreased the feed-to-gain ratio (p < 0.05) during the whole period and significantly enhanced the body weight at week 8 (p < 0.05), 300–400 mg/kg ε-PL significantly increased the fresh pelt weight (p < 0.05). Compared with the control, 300 mg/kg ε-PL significantly increased serum T-SOD activity and jejunal mucosal T-SOD and GSH-Px activities (p < 0.05). Compared to the control, 200–400 mg/kg ε-PL significantly increased serum IgA level (p < 0.05), 300–400 mg/kg ε-PL significantly increased serum IgM level (p < 0.05), 400–500 mg/kg ε-PL significantly increased serum IgG level (p < 0.05). Compared with the control, 200–400 mg/kg ε-PL significantly increased jejunal mucosal IgA level (p < 0.05), 100 mg/kg ε-PL significantly increased jejunal mucosal IgM level (p < 0.05), 100–400 mg/kg ε-PL significantly increased jejunal mucosal IgG level (p < 0.05). Compared with the control group, all ε-PL supplemented groups significantly decreased serum IL-2 and IL-8 levels compared to the control (p < 0.05). The 16S rRNA sequencing analysis revealed that compared to the control, 300 mg/kg ε-PL significantly increased the relative abundance of Firmicutes and Clostridium_sensu_stricto_1 (p < 0.05), and significantly decreased the relative abundance of Proteobacteria and Escherichia-Shigella in ileal mucosa (p < 0.05). Spearman correlation analysis indicated that the relative abundance of Escherichia-Shigella was negatively correlated with the growth performance. The relative abundance of Clostridium_sensu_stricto_1 was positively correlated with the jejunal mucosal antioxidant indicators and immunoglobulin levels.DiscussionIn conclusion, dietary ε-PL supplementation can improve growth performance, antioxidant capacity, immune function, and gut microbial community in growing male minks, and the optimal dosage of ε-PL is 300 mg/kg.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1569620/fullε-polylysineminkgrowth performanceantioxidant capacityimmune functiongut microbiota |
| spellingShingle | Nianxue Wang Heliang Wang Xiaowen Zhang Lianwen Zhao Wenli Li Beibei Zhang Effects of dietary ε-polylysine supplementation on growth performance, antioxidant capacity, immune function, and intestinal microbiota in growing male minks Frontiers in Microbiology ε-polylysine mink growth performance antioxidant capacity immune function gut microbiota |
| title | Effects of dietary ε-polylysine supplementation on growth performance, antioxidant capacity, immune function, and intestinal microbiota in growing male minks |
| title_full | Effects of dietary ε-polylysine supplementation on growth performance, antioxidant capacity, immune function, and intestinal microbiota in growing male minks |
| title_fullStr | Effects of dietary ε-polylysine supplementation on growth performance, antioxidant capacity, immune function, and intestinal microbiota in growing male minks |
| title_full_unstemmed | Effects of dietary ε-polylysine supplementation on growth performance, antioxidant capacity, immune function, and intestinal microbiota in growing male minks |
| title_short | Effects of dietary ε-polylysine supplementation on growth performance, antioxidant capacity, immune function, and intestinal microbiota in growing male minks |
| title_sort | effects of dietary ε polylysine supplementation on growth performance antioxidant capacity immune function and intestinal microbiota in growing male minks |
| topic | ε-polylysine mink growth performance antioxidant capacity immune function gut microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1569620/full |
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