Effect of cadmium and glutathione on malic enzyme activity in brown shrimps (<i>Crangon crangon</i>) from the Gulf of Gdańsk

The high level of cadmium in the abdominal muscle of the brown shrimp <i>Crangon crangon</i> is due to the serious pollution of the water in the Gulf of Gdansk. The inhibition of malic enzyme (ME) activity by cadmium, and in consequence the reduced formation of NADPH, could interfere wit...

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Main Authors: Edward F. Skorkowski, Piotr Stepnowski, Adriana Mika, Anna Białk-Bielińska, Natalia Niedźwiecka
Format: Article
Language:English
Published: Institute of Oceanology of the Polish Academy of Sciences 2011-09-01
Series:Oceanologia
Subjects:
Online Access:http://www.iopan.gda.pl/oceanologia/53_3.html#A7
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author Edward F. Skorkowski
Piotr Stepnowski
Adriana Mika
Anna Białk-Bielińska
Natalia Niedźwiecka
author_facet Edward F. Skorkowski
Piotr Stepnowski
Adriana Mika
Anna Białk-Bielińska
Natalia Niedźwiecka
author_sort Edward F. Skorkowski
collection DOAJ
description The high level of cadmium in the abdominal muscle of the brown shrimp <i>Crangon crangon</i> is due to the serious pollution of the water in the Gulf of Gdansk. The inhibition of malic enzyme (ME) activity by cadmium, and in consequence the reduced formation of NADPH, could interfere with cellular mechanisms for detoxifying the organism and reducing oxidative stress. The reduced glutathione (GSH) concentration in the abdominal muscle of <it>C. crangon</it> was calculated to be 5.8 mM. The objective of this study was to evaluate the part played by GSH in the effect of cadmium on the activity of NADP-dependent malic enzyme from abdominal muscles of brown shrimps. This enzyme is activated by certain divalent cations (Mg, Mn). The results demonstrate that cadmium inhibits ME activity from shrimp muscle, and that GSH and albumin can reduce this cadmium-inhibited NADP-dependent malic enzyme activity.
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publisher Institute of Oceanology of the Polish Academy of Sciences
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spelling doaj-art-302feac5d22f42c7bc97daf62333fcb02025-08-20T03:49:07ZengInstitute of Oceanology of the Polish Academy of SciencesOceanologia0078-32342011-09-01533793805Effect of cadmium and glutathione on malic enzyme activity in brown shrimps (<i>Crangon crangon</i>) from the Gulf of GdańskEdward F. SkorkowskiPiotr StepnowskiAdriana MikaAnna Białk-BielińskaNatalia NiedźwieckaThe high level of cadmium in the abdominal muscle of the brown shrimp <i>Crangon crangon</i> is due to the serious pollution of the water in the Gulf of Gdansk. The inhibition of malic enzyme (ME) activity by cadmium, and in consequence the reduced formation of NADPH, could interfere with cellular mechanisms for detoxifying the organism and reducing oxidative stress. The reduced glutathione (GSH) concentration in the abdominal muscle of <it>C. crangon</it> was calculated to be 5.8 mM. The objective of this study was to evaluate the part played by GSH in the effect of cadmium on the activity of NADP-dependent malic enzyme from abdominal muscles of brown shrimps. This enzyme is activated by certain divalent cations (Mg, Mn). The results demonstrate that cadmium inhibits ME activity from shrimp muscle, and that GSH and albumin can reduce this cadmium-inhibited NADP-dependent malic enzyme activity.http://www.iopan.gda.pl/oceanologia/53_3.html#A7Gulf of GdańskShrimp<i>Crangon crangon</i>Abdomen muscleMalic enzymeCadmiumGlutathione
spellingShingle Edward F. Skorkowski
Piotr Stepnowski
Adriana Mika
Anna Białk-Bielińska
Natalia Niedźwiecka
Effect of cadmium and glutathione on malic enzyme activity in brown shrimps (<i>Crangon crangon</i>) from the Gulf of Gdańsk
Oceanologia
Gulf of Gdańsk
Shrimp
<i>Crangon crangon</i>
Abdomen muscle
Malic enzyme
Cadmium
Glutathione
title Effect of cadmium and glutathione on malic enzyme activity in brown shrimps (<i>Crangon crangon</i>) from the Gulf of Gdańsk
title_full Effect of cadmium and glutathione on malic enzyme activity in brown shrimps (<i>Crangon crangon</i>) from the Gulf of Gdańsk
title_fullStr Effect of cadmium and glutathione on malic enzyme activity in brown shrimps (<i>Crangon crangon</i>) from the Gulf of Gdańsk
title_full_unstemmed Effect of cadmium and glutathione on malic enzyme activity in brown shrimps (<i>Crangon crangon</i>) from the Gulf of Gdańsk
title_short Effect of cadmium and glutathione on malic enzyme activity in brown shrimps (<i>Crangon crangon</i>) from the Gulf of Gdańsk
title_sort effect of cadmium and glutathione on malic enzyme activity in brown shrimps i crangon crangon i from the gulf of gdansk
topic Gulf of Gdańsk
Shrimp
<i>Crangon crangon</i>
Abdomen muscle
Malic enzyme
Cadmium
Glutathione
url http://www.iopan.gda.pl/oceanologia/53_3.html#A7
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