First Record Sparus aurata Larvae in Teluk Penyu Beach Cilacap, Indonesia, revealed by DNA Barcoding

Morphological identification often fails to reveal the taxonomic status of fish larvae. DNA barcoding using particular DNA segment is frequently successful in solving the faulty and might reveal overlooked species, including species outside their recognized geographic ranges, such as Eastern Atlanti...

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Main Authors: Agus Nuryanto, Dian Bhagawati, Elly Tuti Winarni, Hanan Hassan Alsheikh Mahmoud
Format: Article
Language:English
Published: Diponegoro University; Association of Indonesian Coastal Management Experts 2023-12-01
Series:Ilmu Kelautan
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Online Access:https://ejournal.undip.ac.id/index.php/ijms/article/view/53903
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author Agus Nuryanto
Dian Bhagawati
Elly Tuti Winarni
Hanan Hassan Alsheikh Mahmoud
author_facet Agus Nuryanto
Dian Bhagawati
Elly Tuti Winarni
Hanan Hassan Alsheikh Mahmoud
author_sort Agus Nuryanto
collection DOAJ
description Morphological identification often fails to reveal the taxonomic status of fish larvae. DNA barcoding using particular DNA segment is frequently successful in solving the faulty and might reveal overlooked species, including species outside their recognized geographic ranges, such as Eastern Atlantic seabream Sparus aurata. This study aims to assess fish larvae diversity in Teluk Penyu Beach, Cilacap Central Java, Indonesia, through the cytochrome c oxidase 1 barcoding. Fish larvae were collected using a larva net with a diameter of 60 cm and height of 150 cm and horizontal towing during the field trips in March and April 2021. Larvae morphotypes were determined based on their general morphological performance observed under a magnification lens according the available references. Eighteen larvae morphotypes were successfully barcoded, and 5% genetic distance was used as a species border. Fourteen fish larvae species were revealed, with intraspecific genetic distances between 0.00% and 4.12%, while intrageneric genetic distances ranged from 5.50% to 19.29%. An interesting finding was that one larva morphotype was barcoded as S. aurata with high genetic identity (99.19% to 99.68%) and low genetic distance (0.32% to 0.81%). The discovery provides the first new data on S. aurata in Teluk Penyu Beach Cilacap, Central Java, outside its recognized geographic range. Overall, this study provides information about fish larvae in Teluk Penyu Beach, essential for estimating the number and species of fish recruited in the southern Cilacap, which is vital for fisheries management. Nevertheless, new research requires further clarification of S. aurata in Teluk Penyu Beach.
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publishDate 2023-12-01
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spelling doaj-art-302e3e9c9f4c459f9b8eeecac4e17a662025-08-20T03:29:02ZengDiponegoro University; Association of Indonesian Coastal Management ExpertsIlmu Kelautan0853-72912406-75982023-12-0128430131210.14710/ik.ijms.28.4.301-31223141First Record Sparus aurata Larvae in Teluk Penyu Beach Cilacap, Indonesia, revealed by DNA BarcodingAgus Nuryanto0https://orcid.org/0000-0002-7641-0001Dian Bhagawati1Elly Tuti Winarni2Hanan Hassan Alsheikh Mahmoud3Faculty of Biology, Jenderal Soedirman University, IndonesiaFaculty of Biology, Universitas Jenderal Soedirman, IndonesiaFaculty of Biology, Universitas Jenderal Soedirman, IndonesiaCollege of Natural Resources and Environmental Studies, Department of Fisheries Science, University of Bahri, SudanMorphological identification often fails to reveal the taxonomic status of fish larvae. DNA barcoding using particular DNA segment is frequently successful in solving the faulty and might reveal overlooked species, including species outside their recognized geographic ranges, such as Eastern Atlantic seabream Sparus aurata. This study aims to assess fish larvae diversity in Teluk Penyu Beach, Cilacap Central Java, Indonesia, through the cytochrome c oxidase 1 barcoding. Fish larvae were collected using a larva net with a diameter of 60 cm and height of 150 cm and horizontal towing during the field trips in March and April 2021. Larvae morphotypes were determined based on their general morphological performance observed under a magnification lens according the available references. Eighteen larvae morphotypes were successfully barcoded, and 5% genetic distance was used as a species border. Fourteen fish larvae species were revealed, with intraspecific genetic distances between 0.00% and 4.12%, while intrageneric genetic distances ranged from 5.50% to 19.29%. An interesting finding was that one larva morphotype was barcoded as S. aurata with high genetic identity (99.19% to 99.68%) and low genetic distance (0.32% to 0.81%). The discovery provides the first new data on S. aurata in Teluk Penyu Beach Cilacap, Central Java, outside its recognized geographic range. Overall, this study provides information about fish larvae in Teluk Penyu Beach, essential for estimating the number and species of fish recruited in the southern Cilacap, which is vital for fisheries management. Nevertheless, new research requires further clarification of S. aurata in Teluk Penyu Beach.https://ejournal.undip.ac.id/index.php/ijms/article/view/53903barcodingdiversityfish larvaefisheries managementseabream
spellingShingle Agus Nuryanto
Dian Bhagawati
Elly Tuti Winarni
Hanan Hassan Alsheikh Mahmoud
First Record Sparus aurata Larvae in Teluk Penyu Beach Cilacap, Indonesia, revealed by DNA Barcoding
Ilmu Kelautan
barcoding
diversity
fish larvae
fisheries management
seabream
title First Record Sparus aurata Larvae in Teluk Penyu Beach Cilacap, Indonesia, revealed by DNA Barcoding
title_full First Record Sparus aurata Larvae in Teluk Penyu Beach Cilacap, Indonesia, revealed by DNA Barcoding
title_fullStr First Record Sparus aurata Larvae in Teluk Penyu Beach Cilacap, Indonesia, revealed by DNA Barcoding
title_full_unstemmed First Record Sparus aurata Larvae in Teluk Penyu Beach Cilacap, Indonesia, revealed by DNA Barcoding
title_short First Record Sparus aurata Larvae in Teluk Penyu Beach Cilacap, Indonesia, revealed by DNA Barcoding
title_sort first record sparus aurata larvae in teluk penyu beach cilacap indonesia revealed by dna barcoding
topic barcoding
diversity
fish larvae
fisheries management
seabream
url https://ejournal.undip.ac.id/index.php/ijms/article/view/53903
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AT ellytutiwinarni firstrecordsparusauratalarvaeintelukpenyubeachcilacapindonesiarevealedbydnabarcoding
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