DHCR24 overexpression is involved in lipid metabolic reprogramming to drive cervical cancer malignant progression and is associated with immune microenvironment
Abstract Objective Lipid metabolic reprogramming enables tumor cells to acquire malignant phenotypes, including enhanced proliferative capacity, migratory potential, and invasive properties. In cervical cancer, the precise pathobiological roles and molecular mechanisms of DHCR24 remain unclear. Meth...
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2025-08-01
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| Online Access: | https://doi.org/10.1186/s12885-025-14663-2 |
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| author | Lin Cheng Yuance Xu Zhuo Li Danting Sun Yan Xu Junqi He Yulong Chen Qin Yao |
| author_facet | Lin Cheng Yuance Xu Zhuo Li Danting Sun Yan Xu Junqi He Yulong Chen Qin Yao |
| author_sort | Lin Cheng |
| collection | DOAJ |
| description | Abstract Objective Lipid metabolic reprogramming enables tumor cells to acquire malignant phenotypes, including enhanced proliferative capacity, migratory potential, and invasive properties. In cervical cancer, the precise pathobiological roles and molecular mechanisms of DHCR24 remain unclear. Methods We performed comprehensive bioinformatics analyses to investigate the expression patterns, clinical relevance, and prognostic implications of DHCR24 in cervical carcinoma. A prognostic nomogram incorporating tumor stage and DHCR24 expression levels was developed to predict clinical outcomes in patients. Functional studies using SiHa cell lines were conducted to elucidate the oncogenic properties of DHCR24. We used a cholesterol kit to determine the cholesterol content in cervical cancer cells. Finally, we assessed the association between DHCR24 expression and tumor immune microenvironment characteristics through computational analysis of public genomic datasets. Results Our analysis revealed significantly elevated DHCR24 expression in cervical carcinoma specimens, demonstrating statistically significant associations with histological subtype, body mass index (BMI), and therapeutic response (P < 0.05). Univariate and Multivariate Cox regression confirmed DHCR24 as an independent prognostic indicator for cervical cancer. The ROC analysis demonstrates that DHCR24 exhibits robust diagnostic performance for cervical cancer detection. Pharmacological inhibition of DHCR24 using U18666A markedly attenuated oncogenic behaviors, suppressing cellular proliferation, migration, and invasion compared to controls (P < 0.05). Notably, intracellular cholesterol levels exhibited a dose-dependent reduction corresponding to the extent of DHCR24 suppression. Conclusions We hypothesized that DHCR24 is involved in reprogramming lipid metabolism, especially the cholesterol pathway, to promote tumor progression in cervical cancer and correlates with the tumor-infiltrating immune microenvironment. DHCR24 is a biomarker that predicts the progression and prognosis of cervical cancer and is one of the potential targets for cervical cancer therapy. |
| format | Article |
| id | doaj-art-302caf8da0b046d4a3fd83dc8df4f167 |
| institution | DOAJ |
| issn | 1471-2407 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj-art-302caf8da0b046d4a3fd83dc8df4f1672025-08-20T03:05:03ZengBMCBMC Cancer1471-24072025-08-0125111110.1186/s12885-025-14663-2DHCR24 overexpression is involved in lipid metabolic reprogramming to drive cervical cancer malignant progression and is associated with immune microenvironmentLin Cheng0Yuance Xu1Zhuo Li2Danting Sun3Yan Xu4Junqi He5Yulong Chen6Qin Yao7Department of Obstetrics and Gynecology, The Affiliated Hospital of Qingdao University, Qingdao UniversityDepartment of Obstetrics and Gynecology, The Affiliated Hospital of Qingdao University, Qingdao UniversityDepartment of Pediatric Surgery, The Affiliated Hospital of Qingdao University, Qingdao UniversityDepartment of Obstetrics and Gynecology, The Affiliated Hospital of Qingdao University, Qingdao UniversityDepartment of Obstetrics and Gynecology, The Affiliated Hospital of Qingdao University, Qingdao UniversityDepartment of Obstetrics and Gynecology, The Affiliated Hospital of Qingdao University, Qingdao UniversityDepartment of Obstetrics and Gynecology, The Affiliated Hospital of Qingdao University, Qingdao UniversityDepartment of Obstetrics and Gynecology, The Affiliated Hospital of Qingdao University, Qingdao UniversityAbstract Objective Lipid metabolic reprogramming enables tumor cells to acquire malignant phenotypes, including enhanced proliferative capacity, migratory potential, and invasive properties. In cervical cancer, the precise pathobiological roles and molecular mechanisms of DHCR24 remain unclear. Methods We performed comprehensive bioinformatics analyses to investigate the expression patterns, clinical relevance, and prognostic implications of DHCR24 in cervical carcinoma. A prognostic nomogram incorporating tumor stage and DHCR24 expression levels was developed to predict clinical outcomes in patients. Functional studies using SiHa cell lines were conducted to elucidate the oncogenic properties of DHCR24. We used a cholesterol kit to determine the cholesterol content in cervical cancer cells. Finally, we assessed the association between DHCR24 expression and tumor immune microenvironment characteristics through computational analysis of public genomic datasets. Results Our analysis revealed significantly elevated DHCR24 expression in cervical carcinoma specimens, demonstrating statistically significant associations with histological subtype, body mass index (BMI), and therapeutic response (P < 0.05). Univariate and Multivariate Cox regression confirmed DHCR24 as an independent prognostic indicator for cervical cancer. The ROC analysis demonstrates that DHCR24 exhibits robust diagnostic performance for cervical cancer detection. Pharmacological inhibition of DHCR24 using U18666A markedly attenuated oncogenic behaviors, suppressing cellular proliferation, migration, and invasion compared to controls (P < 0.05). Notably, intracellular cholesterol levels exhibited a dose-dependent reduction corresponding to the extent of DHCR24 suppression. Conclusions We hypothesized that DHCR24 is involved in reprogramming lipid metabolism, especially the cholesterol pathway, to promote tumor progression in cervical cancer and correlates with the tumor-infiltrating immune microenvironment. DHCR24 is a biomarker that predicts the progression and prognosis of cervical cancer and is one of the potential targets for cervical cancer therapy.https://doi.org/10.1186/s12885-025-14663-2DHCR24Lipid metabolic reprogrammingCervical cancerImmune microenvironment |
| spellingShingle | Lin Cheng Yuance Xu Zhuo Li Danting Sun Yan Xu Junqi He Yulong Chen Qin Yao DHCR24 overexpression is involved in lipid metabolic reprogramming to drive cervical cancer malignant progression and is associated with immune microenvironment BMC Cancer DHCR24 Lipid metabolic reprogramming Cervical cancer Immune microenvironment |
| title | DHCR24 overexpression is involved in lipid metabolic reprogramming to drive cervical cancer malignant progression and is associated with immune microenvironment |
| title_full | DHCR24 overexpression is involved in lipid metabolic reprogramming to drive cervical cancer malignant progression and is associated with immune microenvironment |
| title_fullStr | DHCR24 overexpression is involved in lipid metabolic reprogramming to drive cervical cancer malignant progression and is associated with immune microenvironment |
| title_full_unstemmed | DHCR24 overexpression is involved in lipid metabolic reprogramming to drive cervical cancer malignant progression and is associated with immune microenvironment |
| title_short | DHCR24 overexpression is involved in lipid metabolic reprogramming to drive cervical cancer malignant progression and is associated with immune microenvironment |
| title_sort | dhcr24 overexpression is involved in lipid metabolic reprogramming to drive cervical cancer malignant progression and is associated with immune microenvironment |
| topic | DHCR24 Lipid metabolic reprogramming Cervical cancer Immune microenvironment |
| url | https://doi.org/10.1186/s12885-025-14663-2 |
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