Anti-obesity functions of fucoidan conducted by bioinformatics and validation findings targeting of autophagy
Autophagy, one of cell death mechanisms that lysosomes degrade endogenous cellular organelles and cytoplasm, is reported to regulate the development of obesity. Fucoidan, a sulfate-containing polysaccharide, is recognized for its anti-obesity effect. In our previous report, we presented the anti-obe...
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Elsevier
2025-03-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893924001890 |
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| author | Jinkai Li Xiaowei Wan Yonghang Li Ping Wang Jian Chen Weihua Jin Jiaqi Liu |
| author_facet | Jinkai Li Xiaowei Wan Yonghang Li Ping Wang Jian Chen Weihua Jin Jiaqi Liu |
| author_sort | Jinkai Li |
| collection | DOAJ |
| description | Autophagy, one of cell death mechanisms that lysosomes degrade endogenous cellular organelles and cytoplasm, is reported to regulate the development of obesity. Fucoidan, a sulfate-containing polysaccharide, is recognized for its anti-obesity effect. In our previous report, we presented the anti-obesity actions of fucoidan, however, the molecular mechanisms targeting autophagy remain unexplored. In this study, the purity, structure, and composition of compounds in fucoidan were characterized by using chemical analysis. Then, we conducted network pharmacology analysis to screen the pharmacological targets and to reveal the molecular mechanisms involved in anti-obesity action of fucoidan through targeting autophagy. Molecularly docked assay was used to further assess the spatial binding capability of fucoidan to target proteins. Our result identified core genes of fucoidan against obesity associated with autophagy. Functional enrichment analysis showed core genes involvement in metabolism, immunity, cell proliferation and differentiation, and kinase activity. Further pathway enrichment analysis chiefly comprised biological binding and immunity. Molecular docking data indicated that the key proteins resulted in potent affinities with fucoidan. Finally, in vitro adipocyte model and animal model were used to validate the preclinical findings that fucoidan suppressed adipocyte proliferation, reduced lipid deposition, induced autophagy, and inhibited adipogenic differentiation. Collectively, these bioinformatics and biochemical findings revealed the anti-obesity effects and mechanisms of fucoidan actions through targeting autophagy. |
| format | Article |
| id | doaj-art-30299f8c013e40a982ccc3cd04bba67b |
| institution | DOAJ |
| issn | 2666-8939 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-30299f8c013e40a982ccc3cd04bba67b2025-08-20T02:55:45ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-03-01910060910.1016/j.carpta.2024.100609Anti-obesity functions of fucoidan conducted by bioinformatics and validation findings targeting of autophagyJinkai Li0Xiaowei Wan1Yonghang Li2Ping Wang3Jian Chen4Weihua Jin5Jiaqi Liu6Key Laboratory of Environmental Pollution and Integrative Omics, Guilin Medical University, Education Department of Guangxi Zhuang Autonomous Region, Guilin, ChinaKey Laboratory of Environmental Pollution and Integrative Omics, Guilin Medical University, Education Department of Guangxi Zhuang Autonomous Region, Guilin, ChinaGuilin Medical University, Guilin, ChinaKey Laboratory of Environmental Pollution and Integrative Omics, Guilin Medical University, Education Department of Guangxi Zhuang Autonomous Region, Guilin, ChinaKey Laboratory of Environmental Pollution and Integrative Omics, Guilin Medical University, Education Department of Guangxi Zhuang Autonomous Region, Guilin, China; Corresponding authors at: Key Laboratory of Environmental Pollution and Integrative Omics, Guilin Medical University, No. 1 Zhiyuan Road, Lingui District, Guilin, China.College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China; Corresponding author at: College of Biotechnology and Bioengineering, Zhejiang University of Technology, No. 18 Chaowang Road, Gongshu District, Hangzhou 310014, Zhejiang, China.Key Laboratory of Environmental Pollution and Integrative Omics, Guilin Medical University, Education Department of Guangxi Zhuang Autonomous Region, Guilin, China; Corresponding authors at: Key Laboratory of Environmental Pollution and Integrative Omics, Guilin Medical University, No. 1 Zhiyuan Road, Lingui District, Guilin, China.Autophagy, one of cell death mechanisms that lysosomes degrade endogenous cellular organelles and cytoplasm, is reported to regulate the development of obesity. Fucoidan, a sulfate-containing polysaccharide, is recognized for its anti-obesity effect. In our previous report, we presented the anti-obesity actions of fucoidan, however, the molecular mechanisms targeting autophagy remain unexplored. In this study, the purity, structure, and composition of compounds in fucoidan were characterized by using chemical analysis. Then, we conducted network pharmacology analysis to screen the pharmacological targets and to reveal the molecular mechanisms involved in anti-obesity action of fucoidan through targeting autophagy. Molecularly docked assay was used to further assess the spatial binding capability of fucoidan to target proteins. Our result identified core genes of fucoidan against obesity associated with autophagy. Functional enrichment analysis showed core genes involvement in metabolism, immunity, cell proliferation and differentiation, and kinase activity. Further pathway enrichment analysis chiefly comprised biological binding and immunity. Molecular docking data indicated that the key proteins resulted in potent affinities with fucoidan. Finally, in vitro adipocyte model and animal model were used to validate the preclinical findings that fucoidan suppressed adipocyte proliferation, reduced lipid deposition, induced autophagy, and inhibited adipogenic differentiation. Collectively, these bioinformatics and biochemical findings revealed the anti-obesity effects and mechanisms of fucoidan actions through targeting autophagy.http://www.sciencedirect.com/science/article/pii/S2666893924001890Structural analysisFucoidanAnti-obesity functional compoundsAutophagyBioinformatics findingsBiochemical validation |
| spellingShingle | Jinkai Li Xiaowei Wan Yonghang Li Ping Wang Jian Chen Weihua Jin Jiaqi Liu Anti-obesity functions of fucoidan conducted by bioinformatics and validation findings targeting of autophagy Carbohydrate Polymer Technologies and Applications Structural analysis Fucoidan Anti-obesity functional compounds Autophagy Bioinformatics findings Biochemical validation |
| title | Anti-obesity functions of fucoidan conducted by bioinformatics and validation findings targeting of autophagy |
| title_full | Anti-obesity functions of fucoidan conducted by bioinformatics and validation findings targeting of autophagy |
| title_fullStr | Anti-obesity functions of fucoidan conducted by bioinformatics and validation findings targeting of autophagy |
| title_full_unstemmed | Anti-obesity functions of fucoidan conducted by bioinformatics and validation findings targeting of autophagy |
| title_short | Anti-obesity functions of fucoidan conducted by bioinformatics and validation findings targeting of autophagy |
| title_sort | anti obesity functions of fucoidan conducted by bioinformatics and validation findings targeting of autophagy |
| topic | Structural analysis Fucoidan Anti-obesity functional compounds Autophagy Bioinformatics findings Biochemical validation |
| url | http://www.sciencedirect.com/science/article/pii/S2666893924001890 |
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