MLPAnalyzer: Data Analysis Tool for Reliable Automated Normalization of MLPA Fragment Data
Background: Multiplex Ligation dependent Probe Amplification (MLPA) is a rapid, simple, reliable and customized method for detection of copy number changes of individual genes at a high resolution and allows for high throughput analysis. This technique is typically applied for studying specific gene...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2008-01-01
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| Series: | Cellular Oncology |
| Online Access: | http://dx.doi.org/10.3233/CLO-2008-0428 |
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| _version_ | 1849699493473157120 |
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| author | Jordy Coffa Mark A. van de Wiel Begoña Diosdado Beatriz Carvalho Jan Schouten Gerrit A. Meijer |
| author_facet | Jordy Coffa Mark A. van de Wiel Begoña Diosdado Beatriz Carvalho Jan Schouten Gerrit A. Meijer |
| author_sort | Jordy Coffa |
| collection | DOAJ |
| description | Background: Multiplex Ligation dependent Probe Amplification (MLPA) is a rapid, simple, reliable and customized method for detection of copy number changes of individual genes at a high resolution and allows for high throughput analysis. This technique is typically applied for studying specific genes in large sample series. The large amount of data, dissimilarities in PCR efficiency among the different probe amplification products, and sample-to-sample variation pose a challenge to data analysis and interpretation. We therefore set out to develop an MLPA data analysis strategy and tool that is simple to use, while still taking into account the above-mentioned sources of variation. |
| format | Article |
| id | doaj-art-301e1c42d8404a068844477cc4aa984d |
| institution | DOAJ |
| issn | 1570-5870 1875-8606 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cellular Oncology |
| spelling | doaj-art-301e1c42d8404a068844477cc4aa984d2025-08-20T03:18:34ZengWileyCellular Oncology1570-58701875-86062008-01-0130432333510.3233/CLO-2008-0428MLPAnalyzer: Data Analysis Tool for Reliable Automated Normalization of MLPA Fragment DataJordy Coffa0Mark A. van de Wiel1Begoña Diosdado2Beatriz Carvalho3Jan Schouten4Gerrit A. Meijer5Tumour Profiling Unit & Micro Array Facility, Department of Pathology (Tumor Profiling Unit), VU University Medical Center, Amsterdam, The NetherlandsTumour Profiling Unit & Micro Array Facility, Department of Pathology (Tumor Profiling Unit), VU University Medical Center, Amsterdam, The NetherlandsTumour Profiling Unit & Micro Array Facility, Department of Pathology (Tumor Profiling Unit), VU University Medical Center, Amsterdam, The NetherlandsTumour Profiling Unit & Micro Array Facility, Department of Pathology (Tumor Profiling Unit), VU University Medical Center, Amsterdam, The NetherlandsMRC-Holland, Amsterdam, The NetherlandsTumour Profiling Unit & Micro Array Facility, Department of Pathology (Tumor Profiling Unit), VU University Medical Center, Amsterdam, The NetherlandsBackground: Multiplex Ligation dependent Probe Amplification (MLPA) is a rapid, simple, reliable and customized method for detection of copy number changes of individual genes at a high resolution and allows for high throughput analysis. This technique is typically applied for studying specific genes in large sample series. The large amount of data, dissimilarities in PCR efficiency among the different probe amplification products, and sample-to-sample variation pose a challenge to data analysis and interpretation. We therefore set out to develop an MLPA data analysis strategy and tool that is simple to use, while still taking into account the above-mentioned sources of variation.http://dx.doi.org/10.3233/CLO-2008-0428 |
| spellingShingle | Jordy Coffa Mark A. van de Wiel Begoña Diosdado Beatriz Carvalho Jan Schouten Gerrit A. Meijer MLPAnalyzer: Data Analysis Tool for Reliable Automated Normalization of MLPA Fragment Data Cellular Oncology |
| title | MLPAnalyzer: Data Analysis Tool for Reliable Automated Normalization of MLPA Fragment Data |
| title_full | MLPAnalyzer: Data Analysis Tool for Reliable Automated Normalization of MLPA Fragment Data |
| title_fullStr | MLPAnalyzer: Data Analysis Tool for Reliable Automated Normalization of MLPA Fragment Data |
| title_full_unstemmed | MLPAnalyzer: Data Analysis Tool for Reliable Automated Normalization of MLPA Fragment Data |
| title_short | MLPAnalyzer: Data Analysis Tool for Reliable Automated Normalization of MLPA Fragment Data |
| title_sort | mlpanalyzer data analysis tool for reliable automated normalization of mlpa fragment data |
| url | http://dx.doi.org/10.3233/CLO-2008-0428 |
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