Full-length transcriptome sequencing of seven tissues of GuShi chickens
Chickens are vital economic poultry and serve as exemplary models for avian research. The incomplete reference genome of chickens and the limited availability of full-length cDNA impede the identification of alternatively spliced transcripts, thereby delaying many fundamental chicken studies. We uti...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124012756 |
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| author | Kaiyuan Tian Chenxi Zhang Chaoqun Gao Junlai Shi Chunhong Xu Wanying Xie Sensen Yan Chengpeng Xiao Xintao Jia Yixiang Tian Guirong Sun Xiangtao Kang Kejun Wang Wenting Li |
| author_facet | Kaiyuan Tian Chenxi Zhang Chaoqun Gao Junlai Shi Chunhong Xu Wanying Xie Sensen Yan Chengpeng Xiao Xintao Jia Yixiang Tian Guirong Sun Xiangtao Kang Kejun Wang Wenting Li |
| author_sort | Kaiyuan Tian |
| collection | DOAJ |
| description | Chickens are vital economic poultry and serve as exemplary models for avian research. The incomplete reference genome of chickens and the limited availability of full-length cDNA impede the identification of alternatively spliced transcripts, thereby delaying many fundamental chicken studies. We utilized PacBio Iso-seq technology on various chicken tissues, obtaining 170,162 full-length transcripts through comprehensive transcriptome sequencing and annotation. Of the identified transcripts, 38,925 lncRNAs were predicted and categorized into five models. Our data significantly increases the known number of chickens’ lncRNAs and alternatively spliced transcripts are crucial in enhancing current genome annotations. Furthermore, this data will be invaluable for functional studies of other avian species. |
| format | Article |
| id | doaj-art-3017f695b5ec4e7aa5662393103c6ea9 |
| institution | OA Journals |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-3017f695b5ec4e7aa5662393103c6ea92025-08-20T02:39:08ZengElsevierPoultry Science0032-57912025-02-01104210469710.1016/j.psj.2024.104697Full-length transcriptome sequencing of seven tissues of GuShi chickensKaiyuan Tian0Chenxi Zhang1Chaoqun Gao2Junlai Shi3Chunhong Xu4Wanying Xie5Sensen Yan6Chengpeng Xiao7Xintao Jia8Yixiang Tian9Guirong Sun10Xiangtao Kang11Kejun Wang12Wenting Li13College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China.; The Shennong Laboratory, Zhengzhou 450002, PR China.College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China.; The Shennong Laboratory, Zhengzhou 450002, PR China.College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China.; The Shennong Laboratory, Zhengzhou 450002, PR China.College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China.; The Shennong Laboratory, Zhengzhou 450002, PR China.College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China.; The Shennong Laboratory, Zhengzhou 450002, PR China.College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China.; The Shennong Laboratory, Zhengzhou 450002, PR China.College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China.; The Shennong Laboratory, Zhengzhou 450002, PR China.College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China.; The Shennong Laboratory, Zhengzhou 450002, PR China.College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China.; The Shennong Laboratory, Zhengzhou 450002, PR China.College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China.; The Shennong Laboratory, Zhengzhou 450002, PR China.College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China.; The Shennong Laboratory, Zhengzhou 450002, PR China.College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China.; The Shennong Laboratory, Zhengzhou 450002, PR China.College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China.; The Shennong Laboratory, Zhengzhou 450002, PR China.College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, PR China.; The Shennong Laboratory, Zhengzhou 450002, PR China.; Correspondence author at: Henan Agricultural University, Zhengzhou City, Henan Province.Chickens are vital economic poultry and serve as exemplary models for avian research. The incomplete reference genome of chickens and the limited availability of full-length cDNA impede the identification of alternatively spliced transcripts, thereby delaying many fundamental chicken studies. We utilized PacBio Iso-seq technology on various chicken tissues, obtaining 170,162 full-length transcripts through comprehensive transcriptome sequencing and annotation. Of the identified transcripts, 38,925 lncRNAs were predicted and categorized into five models. Our data significantly increases the known number of chickens’ lncRNAs and alternatively spliced transcripts are crucial in enhancing current genome annotations. Furthermore, this data will be invaluable for functional studies of other avian species.http://www.sciencedirect.com/science/article/pii/S0032579124012756Full-length transcriptAlternative splicingFusion genetranscript structure |
| spellingShingle | Kaiyuan Tian Chenxi Zhang Chaoqun Gao Junlai Shi Chunhong Xu Wanying Xie Sensen Yan Chengpeng Xiao Xintao Jia Yixiang Tian Guirong Sun Xiangtao Kang Kejun Wang Wenting Li Full-length transcriptome sequencing of seven tissues of GuShi chickens Poultry Science Full-length transcript Alternative splicing Fusion gene transcript structure |
| title | Full-length transcriptome sequencing of seven tissues of GuShi chickens |
| title_full | Full-length transcriptome sequencing of seven tissues of GuShi chickens |
| title_fullStr | Full-length transcriptome sequencing of seven tissues of GuShi chickens |
| title_full_unstemmed | Full-length transcriptome sequencing of seven tissues of GuShi chickens |
| title_short | Full-length transcriptome sequencing of seven tissues of GuShi chickens |
| title_sort | full length transcriptome sequencing of seven tissues of gushi chickens |
| topic | Full-length transcript Alternative splicing Fusion gene transcript structure |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124012756 |
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