Development of a Novel Sensor System Based on Magnetic Microspheres to Detect Cardiac Troponin T
Acute myocardial infarction (AMI) causes irreversible injury to cardiomyocytes in a short time and may result in various complications, severely threatening patient safety. Therefore, it is necessary to predict the possibility of AMI in the prophase. Prognostic detection of biomarkers that specifica...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2020/8855550 |
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| _version_ | 1832554554256785408 |
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| author | Junrong Zhang Hongxiang Liu Baofeng Xu Sijun Huang Rui Liu Jinming Zhu Yi Guo Li Xu |
| author_facet | Junrong Zhang Hongxiang Liu Baofeng Xu Sijun Huang Rui Liu Jinming Zhu Yi Guo Li Xu |
| author_sort | Junrong Zhang |
| collection | DOAJ |
| description | Acute myocardial infarction (AMI) causes irreversible injury to cardiomyocytes in a short time and may result in various complications, severely threatening patient safety. Therefore, it is necessary to predict the possibility of AMI in the prophase. Prognostic detection of biomarkers that specifically reflect myocardial damage in a patient’s blood has become an essential mediating measure to prevent the serious occurrence of AMI. The present study is aimed at exploring a novel sensing system with high specificity and precision based on magnetic microspheres developed to detect cardiac troponin T (cTnT), which is the most specific diagnostic marker for AMI in cardiovascular diseases. Naive human cTnT protein in serum samples and antigens on functional magnetic microspheres will competitively bind with limited specific antibodies. After rapid removal of heterogeneous elements in the sera using a magnetic separator, fluorescein isothiocyanate-labeled immunoglobulin G is added to react with specific antibodies on the magnetic microspheres. Then, a flow cytometer is used to collect signals of different fluorescence intensities. The results show that the method is characterized by economy, high accuracy, and novelty. It can be used for the detection of cTnT in blood at 1.7–106.1 ng/mL, with a detection limit of 0.5 ng/mL. Thus, the proposed sensor improves the accuracy and efficiency of diagnosis before clinical deterioration of AMI. |
| format | Article |
| id | doaj-art-301500bcdf244d009007736f621143d4 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-301500bcdf244d009007736f621143d42025-02-03T05:51:15ZengWileyInternational Journal of Polymer Science1687-94221687-94302020-01-01202010.1155/2020/88555508855550Development of a Novel Sensor System Based on Magnetic Microspheres to Detect Cardiac Troponin TJunrong Zhang0Hongxiang Liu1Baofeng Xu2Sijun Huang3Rui Liu4Jinming Zhu5Yi Guo6Li Xu7Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, ChinaKey Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, ChinaDepartment of Neurosurgery, The First Hospital of Jilin University, Changchun 130021, ChinaKey Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, ChinaChina-Japan Union Hospital of Jilin University, Changchun 130033, ChinaChina-Japan Union Hospital of Jilin University, Changchun 130033, ChinaKey Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, ChinaKey Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, ChinaAcute myocardial infarction (AMI) causes irreversible injury to cardiomyocytes in a short time and may result in various complications, severely threatening patient safety. Therefore, it is necessary to predict the possibility of AMI in the prophase. Prognostic detection of biomarkers that specifically reflect myocardial damage in a patient’s blood has become an essential mediating measure to prevent the serious occurrence of AMI. The present study is aimed at exploring a novel sensing system with high specificity and precision based on magnetic microspheres developed to detect cardiac troponin T (cTnT), which is the most specific diagnostic marker for AMI in cardiovascular diseases. Naive human cTnT protein in serum samples and antigens on functional magnetic microspheres will competitively bind with limited specific antibodies. After rapid removal of heterogeneous elements in the sera using a magnetic separator, fluorescein isothiocyanate-labeled immunoglobulin G is added to react with specific antibodies on the magnetic microspheres. Then, a flow cytometer is used to collect signals of different fluorescence intensities. The results show that the method is characterized by economy, high accuracy, and novelty. It can be used for the detection of cTnT in blood at 1.7–106.1 ng/mL, with a detection limit of 0.5 ng/mL. Thus, the proposed sensor improves the accuracy and efficiency of diagnosis before clinical deterioration of AMI.http://dx.doi.org/10.1155/2020/8855550 |
| spellingShingle | Junrong Zhang Hongxiang Liu Baofeng Xu Sijun Huang Rui Liu Jinming Zhu Yi Guo Li Xu Development of a Novel Sensor System Based on Magnetic Microspheres to Detect Cardiac Troponin T International Journal of Polymer Science |
| title | Development of a Novel Sensor System Based on Magnetic Microspheres to Detect Cardiac Troponin T |
| title_full | Development of a Novel Sensor System Based on Magnetic Microspheres to Detect Cardiac Troponin T |
| title_fullStr | Development of a Novel Sensor System Based on Magnetic Microspheres to Detect Cardiac Troponin T |
| title_full_unstemmed | Development of a Novel Sensor System Based on Magnetic Microspheres to Detect Cardiac Troponin T |
| title_short | Development of a Novel Sensor System Based on Magnetic Microspheres to Detect Cardiac Troponin T |
| title_sort | development of a novel sensor system based on magnetic microspheres to detect cardiac troponin t |
| url | http://dx.doi.org/10.1155/2020/8855550 |
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