Toward Personalized Immunotherapeutic Drug Monitoring with Multiplexed Extended‐Gate Field‐Effect‐Transistor Biosensors
The selection and optimization of therapies for cancer patients urgently need personalization. Portable point‐of‐care electronic biosensors emerge as a groundbreaking solution contributing to better decision‐making in precision oncology. In this study, the innovative use of extended‐gate field‐effec...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2025-05-01
|
| Series: | Small Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/smsc.202400515 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850206325135376384 |
|---|---|
| author | Trang‐Anh Nguyen‐Le Christin Neuber Isli Cela Željko Janićijević Liliana Rodrigues Loureiro Lydia Hoffmann Anja Feldmann Michael Bachmann Larysa Baraban |
| author_facet | Trang‐Anh Nguyen‐Le Christin Neuber Isli Cela Željko Janićijević Liliana Rodrigues Loureiro Lydia Hoffmann Anja Feldmann Michael Bachmann Larysa Baraban |
| author_sort | Trang‐Anh Nguyen‐Le |
| collection | DOAJ |
| description | The selection and optimization of therapies for cancer patients urgently need personalization. Portable point‐of‐care electronic biosensors emerge as a groundbreaking solution contributing to better decision‐making in precision oncology. In this study, the innovative use of extended‐gate field‐effect‐transistor (EG‐FET) biosensors is showcased for monitoring the concentration and pharmacokinetics of immunotherapeutic drugs in vivo. Complementary positron emission tomography and radioactivity biodistribution studies in mice validate the EG‐FET measurements. Herein, a novel indirect assay format is also introduced for detecting target modules (TMs) in an adapter chimeric antigen receptor T‐cell therapy model, effectively addressing the current limitations of potentiometric measurements. In pharmacokinetic evaluations, the EG‐FET biosensor performance aligns with standard radioactivity measurements, revealing the distinct lifespans of small‐sized single‐chain‐fragment‐variable‐derived TMs (15 min) and larger IgG4‐derived TMs (14 h). Advantageously, the EG‐FET sensors exhibit exceptional sensitivity and fulfill the requirements for immunotherapeutic drug monitoring without complex radioactive labeling, which is indispensable. In these promising findings, the exploration of next‐generation electronic biosensors as therapeutic monitoring tools is advocated for. With their cost, size, and response time advantages, these biosensors hold immense potential for advancing personalized oncology, transcending the conventional diagnostic roles typically highlighted in the literature. |
| format | Article |
| id | doaj-art-3011afc907c14b5bba4e5d256c7a45f3 |
| institution | OA Journals |
| issn | 2688-4046 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Small Science |
| spelling | doaj-art-3011afc907c14b5bba4e5d256c7a45f32025-08-20T02:10:52ZengWiley-VCHSmall Science2688-40462025-05-0155n/an/a10.1002/smsc.202400515Toward Personalized Immunotherapeutic Drug Monitoring with Multiplexed Extended‐Gate Field‐Effect‐Transistor BiosensorsTrang‐Anh Nguyen‐Le0Christin Neuber1Isli Cela2Željko Janićijević3Liliana Rodrigues Loureiro4Lydia Hoffmann5Anja Feldmann6Michael Bachmann7Larysa Baraban8Institute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. (HZDR) 01328 Dresden GermanyInstitute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. (HZDR) 01328 Dresden GermanyInstitute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. (HZDR) 01328 Dresden GermanyInstitute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. (HZDR) 01328 Dresden GermanyInstitute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. (HZDR) 01328 Dresden GermanyInstitute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. (HZDR) 01328 Dresden GermanyInstitute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. (HZDR) 01328 Dresden GermanyInstitute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. (HZDR) 01328 Dresden GermanyInstitute of Radiopharmaceutical Cancer Research Helmholtz‐Zentrum Dresden‐Rossendorf e. V. (HZDR) 01328 Dresden GermanyThe selection and optimization of therapies for cancer patients urgently need personalization. Portable point‐of‐care electronic biosensors emerge as a groundbreaking solution contributing to better decision‐making in precision oncology. In this study, the innovative use of extended‐gate field‐effect‐transistor (EG‐FET) biosensors is showcased for monitoring the concentration and pharmacokinetics of immunotherapeutic drugs in vivo. Complementary positron emission tomography and radioactivity biodistribution studies in mice validate the EG‐FET measurements. Herein, a novel indirect assay format is also introduced for detecting target modules (TMs) in an adapter chimeric antigen receptor T‐cell therapy model, effectively addressing the current limitations of potentiometric measurements. In pharmacokinetic evaluations, the EG‐FET biosensor performance aligns with standard radioactivity measurements, revealing the distinct lifespans of small‐sized single‐chain‐fragment‐variable‐derived TMs (15 min) and larger IgG4‐derived TMs (14 h). Advantageously, the EG‐FET sensors exhibit exceptional sensitivity and fulfill the requirements for immunotherapeutic drug monitoring without complex radioactive labeling, which is indispensable. In these promising findings, the exploration of next‐generation electronic biosensors as therapeutic monitoring tools is advocated for. With their cost, size, and response time advantages, these biosensors hold immense potential for advancing personalized oncology, transcending the conventional diagnostic roles typically highlighted in the literature.https://doi.org/10.1002/smsc.202400515biosensorsextended gatefield‐effect‐transistorimmunosensorsimmunotherapiespoint‐of‐care |
| spellingShingle | Trang‐Anh Nguyen‐Le Christin Neuber Isli Cela Željko Janićijević Liliana Rodrigues Loureiro Lydia Hoffmann Anja Feldmann Michael Bachmann Larysa Baraban Toward Personalized Immunotherapeutic Drug Monitoring with Multiplexed Extended‐Gate Field‐Effect‐Transistor Biosensors Small Science biosensors extended gate field‐effect‐transistor immunosensors immunotherapies point‐of‐care |
| title | Toward Personalized Immunotherapeutic Drug Monitoring with Multiplexed Extended‐Gate Field‐Effect‐Transistor Biosensors |
| title_full | Toward Personalized Immunotherapeutic Drug Monitoring with Multiplexed Extended‐Gate Field‐Effect‐Transistor Biosensors |
| title_fullStr | Toward Personalized Immunotherapeutic Drug Monitoring with Multiplexed Extended‐Gate Field‐Effect‐Transistor Biosensors |
| title_full_unstemmed | Toward Personalized Immunotherapeutic Drug Monitoring with Multiplexed Extended‐Gate Field‐Effect‐Transistor Biosensors |
| title_short | Toward Personalized Immunotherapeutic Drug Monitoring with Multiplexed Extended‐Gate Field‐Effect‐Transistor Biosensors |
| title_sort | toward personalized immunotherapeutic drug monitoring with multiplexed extended gate field effect transistor biosensors |
| topic | biosensors extended gate field‐effect‐transistor immunosensors immunotherapies point‐of‐care |
| url | https://doi.org/10.1002/smsc.202400515 |
| work_keys_str_mv | AT tranganhnguyenle towardpersonalizedimmunotherapeuticdrugmonitoringwithmultiplexedextendedgatefieldeffecttransistorbiosensors AT christinneuber towardpersonalizedimmunotherapeuticdrugmonitoringwithmultiplexedextendedgatefieldeffecttransistorbiosensors AT islicela towardpersonalizedimmunotherapeuticdrugmonitoringwithmultiplexedextendedgatefieldeffecttransistorbiosensors AT zeljkojanicijevic towardpersonalizedimmunotherapeuticdrugmonitoringwithmultiplexedextendedgatefieldeffecttransistorbiosensors AT lilianarodriguesloureiro towardpersonalizedimmunotherapeuticdrugmonitoringwithmultiplexedextendedgatefieldeffecttransistorbiosensors AT lydiahoffmann towardpersonalizedimmunotherapeuticdrugmonitoringwithmultiplexedextendedgatefieldeffecttransistorbiosensors AT anjafeldmann towardpersonalizedimmunotherapeuticdrugmonitoringwithmultiplexedextendedgatefieldeffecttransistorbiosensors AT michaelbachmann towardpersonalizedimmunotherapeuticdrugmonitoringwithmultiplexedextendedgatefieldeffecttransistorbiosensors AT larysabaraban towardpersonalizedimmunotherapeuticdrugmonitoringwithmultiplexedextendedgatefieldeffecttransistorbiosensors |