Elevated OPN, IP-10, and Neutrophilia in Loop-Mediated Isothermal Amplification Confirmed Tuberculosis Patients
Tuberculosis (TB) is the second most common cause of death from infectious diseases and results in high socioeconomic losses to many countries. Proper diagnosis is the first step in TB eradication. To develop a rapid, simple, and accurate diagnostic TB test and to characterize the prevalence of Myco...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/513263 |
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| author | Beata Shiratori Susan Leano Chie Nakajima Haorile Chagan-Yasutan Toshiro Niki Yugo Ashino Yasuhiko Suzuki Elisabeth Telan Toshio Hattori |
| author_facet | Beata Shiratori Susan Leano Chie Nakajima Haorile Chagan-Yasutan Toshiro Niki Yugo Ashino Yasuhiko Suzuki Elisabeth Telan Toshio Hattori |
| author_sort | Beata Shiratori |
| collection | DOAJ |
| description | Tuberculosis (TB) is the second most common cause of death from infectious diseases and results in high socioeconomic losses to many countries. Proper diagnosis is the first step in TB eradication. To develop a rapid, simple, and accurate diagnostic TB test and to characterize the prevalence of Mycobacterium tuberculosis (MTB) genotypes and immune profiles of TB patients, a total of 37 TB patients and 30 healthy control (HC) from Metro Manila were enrolled. Loop-mediated isothermal amplification (LAMP) reliably detected MTB infection. Manila genotype was identified by spoligotyping method in all TB patients. Osteopontin (OPN), interferon-γ-induced protein 10 kDa (IP-10), and neutrophil counts were found to reflect the acute stage of MTB infection. The sensitivity and specificity were 94.6% and 93.3%, respectively, for both OPN and IP-10, and they were 83.8% and 78.6%, respectively, for neutrophils. The combination of OPN, IP-10, neutrophil count, IL-6, IL-8, TNF-α, MCP-1, platelets, galectin-9, and leukocyte count correctly identifies all the HC and 96.3% of TB patients. LAMP method may serve as a rapid, supportive method in addition to time-consuming culture methods. OPN, IP-10, and neutrophil counts are useful in detecting MTB infection and may have utility in monitoring the course of the disease. |
| format | Article |
| id | doaj-art-300d67f92b344469bcec784ac245b034 |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-300d67f92b344469bcec784ac245b0342025-08-20T03:20:23ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/513263513263Elevated OPN, IP-10, and Neutrophilia in Loop-Mediated Isothermal Amplification Confirmed Tuberculosis PatientsBeata Shiratori0Susan Leano1Chie Nakajima2Haorile Chagan-Yasutan3Toshiro Niki4Yugo Ashino5Yasuhiko Suzuki6Elisabeth Telan7Toshio Hattori8Division of Disaster-Related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, JapanSTD AIDS Cooperative Central Laboratory, San Lazaro Hospital, Quiricada Street, 1003 Manila, PhilippinesDivision of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, North 20, West 10 Kita-ku, Sapporo 001-0020, JapanDivision of Disaster-Related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, JapanResearch Division, GalPharma Company, Ltd., NEXT-Kagawa 204, 2217-44 Hayashi-cho, Takamatsu-shi, Kagawa 760-0301, JapanDivision of Disaster-Related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, JapanDivision of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, North 20, West 10 Kita-ku, Sapporo 001-0020, JapanSTD AIDS Cooperative Central Laboratory, San Lazaro Hospital, Quiricada Street, 1003 Manila, PhilippinesDivision of Disaster-Related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, JapanTuberculosis (TB) is the second most common cause of death from infectious diseases and results in high socioeconomic losses to many countries. Proper diagnosis is the first step in TB eradication. To develop a rapid, simple, and accurate diagnostic TB test and to characterize the prevalence of Mycobacterium tuberculosis (MTB) genotypes and immune profiles of TB patients, a total of 37 TB patients and 30 healthy control (HC) from Metro Manila were enrolled. Loop-mediated isothermal amplification (LAMP) reliably detected MTB infection. Manila genotype was identified by spoligotyping method in all TB patients. Osteopontin (OPN), interferon-γ-induced protein 10 kDa (IP-10), and neutrophil counts were found to reflect the acute stage of MTB infection. The sensitivity and specificity were 94.6% and 93.3%, respectively, for both OPN and IP-10, and they were 83.8% and 78.6%, respectively, for neutrophils. The combination of OPN, IP-10, neutrophil count, IL-6, IL-8, TNF-α, MCP-1, platelets, galectin-9, and leukocyte count correctly identifies all the HC and 96.3% of TB patients. LAMP method may serve as a rapid, supportive method in addition to time-consuming culture methods. OPN, IP-10, and neutrophil counts are useful in detecting MTB infection and may have utility in monitoring the course of the disease.http://dx.doi.org/10.1155/2014/513263 |
| spellingShingle | Beata Shiratori Susan Leano Chie Nakajima Haorile Chagan-Yasutan Toshiro Niki Yugo Ashino Yasuhiko Suzuki Elisabeth Telan Toshio Hattori Elevated OPN, IP-10, and Neutrophilia in Loop-Mediated Isothermal Amplification Confirmed Tuberculosis Patients Mediators of Inflammation |
| title | Elevated OPN, IP-10, and Neutrophilia in Loop-Mediated Isothermal Amplification Confirmed Tuberculosis Patients |
| title_full | Elevated OPN, IP-10, and Neutrophilia in Loop-Mediated Isothermal Amplification Confirmed Tuberculosis Patients |
| title_fullStr | Elevated OPN, IP-10, and Neutrophilia in Loop-Mediated Isothermal Amplification Confirmed Tuberculosis Patients |
| title_full_unstemmed | Elevated OPN, IP-10, and Neutrophilia in Loop-Mediated Isothermal Amplification Confirmed Tuberculosis Patients |
| title_short | Elevated OPN, IP-10, and Neutrophilia in Loop-Mediated Isothermal Amplification Confirmed Tuberculosis Patients |
| title_sort | elevated opn ip 10 and neutrophilia in loop mediated isothermal amplification confirmed tuberculosis patients |
| url | http://dx.doi.org/10.1155/2014/513263 |
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