Regulation of immune microenvironments by polyetheretherketone surface topography for improving osseointegration
Abstract Optimizing the immune microenvironment is essential for successful implant osseointegration. In this study, four different nano/microstructures were fabricated on polyetheretherketone (PEEK) substrates by varying the agitation speed during sulfonation to influence osteoimmunomodulation and...
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| Format: | Article |
| Language: | English |
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BMC
2025-03-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-025-03272-7 |
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| author | Yuqing Zhao Yanxin An Fan Wu Lipeng Liu Franklin R. Tay Yang Jiao Jing Wang |
| author_facet | Yuqing Zhao Yanxin An Fan Wu Lipeng Liu Franklin R. Tay Yang Jiao Jing Wang |
| author_sort | Yuqing Zhao |
| collection | DOAJ |
| description | Abstract Optimizing the immune microenvironment is essential for successful implant osseointegration. In this study, four different nano/microstructures were fabricated on polyetheretherketone (PEEK) substrates by varying the agitation speed during sulfonation to influence osteoimmunomodulation and implant integration. The results indicate that nano/microstructures with minimal dimensions (SP450) inhibit actin polymerization by reducing calcium influx through PIEZO1, activating the anti-inflammatory M2 macrophage phenotype. Among the tested specimens, SP450 exhibited the lowest expression levels of tumor necrosis factor-α and interleukin-1β while releasing the highest levels of anti-inflammatory factors, including interleukin-4 and interleukin-10. This optimized immune environment promotes the osteogenesis of MC3T3-E1 pre-osteoblasts and enhances the osseointegration of PEEK implants. Transcriptomic analysis and validation experiment further revealed that SP450 inhibits osteoclastic differentiation by down-regulating transforming growth factor-β2 and suppressing the NF-κB signaling pathway. These findings suggest that manipulating the surface topography of PEEK implants is an effective strategy for enhancing osseointegration with promising clinical applications. |
| format | Article |
| id | doaj-art-2ffde6c66b5842dc98328476604f48da |
| institution | DOAJ |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-2ffde6c66b5842dc98328476604f48da2025-08-20T03:02:19ZengBMCJournal of Nanobiotechnology1477-31552025-03-0123112210.1186/s12951-025-03272-7Regulation of immune microenvironments by polyetheretherketone surface topography for improving osseointegrationYuqing Zhao0Yanxin An1Fan Wu2Lipeng Liu3Franklin R. Tay4Yang Jiao5Jing Wang6State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Oral Implants, School of Stomatology, The Fourth Military Medical UniversityDepartment of General Surgery, The First Affiliated Hospital of Xi’an Medical UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Oral Implants, School of Stomatology, The Fourth Military Medical UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Clinical Research Center for Oral Diseases, Department of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical UniversityThe Graduate School, Augusta UniversityDepartment of Stomatology, The Seventh Medical Center of PLA General HospitalState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Engineering Research Center for Dental Materials and Advanced Manufacture, Department of Oral Implants, School of Stomatology, The Fourth Military Medical UniversityAbstract Optimizing the immune microenvironment is essential for successful implant osseointegration. In this study, four different nano/microstructures were fabricated on polyetheretherketone (PEEK) substrates by varying the agitation speed during sulfonation to influence osteoimmunomodulation and implant integration. The results indicate that nano/microstructures with minimal dimensions (SP450) inhibit actin polymerization by reducing calcium influx through PIEZO1, activating the anti-inflammatory M2 macrophage phenotype. Among the tested specimens, SP450 exhibited the lowest expression levels of tumor necrosis factor-α and interleukin-1β while releasing the highest levels of anti-inflammatory factors, including interleukin-4 and interleukin-10. This optimized immune environment promotes the osteogenesis of MC3T3-E1 pre-osteoblasts and enhances the osseointegration of PEEK implants. Transcriptomic analysis and validation experiment further revealed that SP450 inhibits osteoclastic differentiation by down-regulating transforming growth factor-β2 and suppressing the NF-κB signaling pathway. These findings suggest that manipulating the surface topography of PEEK implants is an effective strategy for enhancing osseointegration with promising clinical applications.https://doi.org/10.1186/s12951-025-03272-7Macrophage polarizationNano/microstructuresOsteoimmunomodulationOsseointegrationPolyetheretherketone |
| spellingShingle | Yuqing Zhao Yanxin An Fan Wu Lipeng Liu Franklin R. Tay Yang Jiao Jing Wang Regulation of immune microenvironments by polyetheretherketone surface topography for improving osseointegration Journal of Nanobiotechnology Macrophage polarization Nano/microstructures Osteoimmunomodulation Osseointegration Polyetheretherketone |
| title | Regulation of immune microenvironments by polyetheretherketone surface topography for improving osseointegration |
| title_full | Regulation of immune microenvironments by polyetheretherketone surface topography for improving osseointegration |
| title_fullStr | Regulation of immune microenvironments by polyetheretherketone surface topography for improving osseointegration |
| title_full_unstemmed | Regulation of immune microenvironments by polyetheretherketone surface topography for improving osseointegration |
| title_short | Regulation of immune microenvironments by polyetheretherketone surface topography for improving osseointegration |
| title_sort | regulation of immune microenvironments by polyetheretherketone surface topography for improving osseointegration |
| topic | Macrophage polarization Nano/microstructures Osteoimmunomodulation Osseointegration Polyetheretherketone |
| url | https://doi.org/10.1186/s12951-025-03272-7 |
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