Distinct Immune Homeostasis Remodeling Patterns after HLA‐Matched and Haploidentical Transplantation

Abstract Allogeneic hematopoietic stem cell transplantation (allo‐HSCT) is a widely used treatment for a variety of hematopoietic disorders, and also provides a valuable platform for investigating the development of donor‐derived immune cells in recipients post‐HSCT. The immune system remodels from...

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Main Authors: Huidong Guo, Liping Guo, Bixia Wang, Xinya Jiang, Zhigui Wu, Xiao‐Dong Mo, Yu‐Qian Sun, Yuan‐Yuan Zhang, Zhi‐Dong Wang, Jun Kong, Chen‐Hua Yan, Xiao‐Jun Huang
Format: Article
Language:English
Published: Wiley 2024-10-01
Series:Advanced Science
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Online Access:https://doi.org/10.1002/advs.202400544
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author Huidong Guo
Liping Guo
Bixia Wang
Xinya Jiang
Zhigui Wu
Xiao‐Dong Mo
Yu‐Qian Sun
Yuan‐Yuan Zhang
Zhi‐Dong Wang
Jun Kong
Chen‐Hua Yan
Xiao‐Jun Huang
author_facet Huidong Guo
Liping Guo
Bixia Wang
Xinya Jiang
Zhigui Wu
Xiao‐Dong Mo
Yu‐Qian Sun
Yuan‐Yuan Zhang
Zhi‐Dong Wang
Jun Kong
Chen‐Hua Yan
Xiao‐Jun Huang
author_sort Huidong Guo
collection DOAJ
description Abstract Allogeneic hematopoietic stem cell transplantation (allo‐HSCT) is a widely used treatment for a variety of hematopoietic disorders, and also provides a valuable platform for investigating the development of donor‐derived immune cells in recipients post‐HSCT. The immune system remodels from the donor to the recipient during allo‐HSCT. However, little is known about the cell profile alterations as donor homeostasis rebalances to recipient homeostasis following HSCT. Here, multi‐omics technology is applied at both the single cell and bulk sample levels, as well as spectrum flow cytometry and fluorescent transgenic mouse models, to dissect the dynamics of the rebalanced homeostatic immune system in recipients after allo‐HSCT. The data reveal that all immune subpopulations observed in donors are successfully restored in recipients, though with varying levels of abundance. The remodeling of immune homeostasis exhibits different patterns in HLA‐matched and haploidentical HSCT, highlighting distinct biases in T cell reconstitution from the central and peripheral pathways. Furthermore, ZNF683 is critical for maintaining the persistence and quiescence of CD8 T‐cell in haploidentical HSCT. The research can serve as a foundation for developing novel strategies to induce immune tolerance.
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spelling doaj-art-2ffdbc9ed3464a97b8aebab9b894ef352025-08-20T02:10:42ZengWileyAdvanced Science2198-38442024-10-011139n/an/a10.1002/advs.202400544Distinct Immune Homeostasis Remodeling Patterns after HLA‐Matched and Haploidentical TransplantationHuidong Guo0Liping Guo1Bixia Wang2Xinya Jiang3Zhigui Wu4Xiao‐Dong Mo5Yu‐Qian Sun6Yuan‐Yuan Zhang7Zhi‐Dong Wang8Jun Kong9Chen‐Hua Yan10Xiao‐Jun Huang11National Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Peking University People's Hospital Peking University Institute of Hematology Peking University Beijing 100044 ChinaNational Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Peking University People's Hospital Peking University Institute of Hematology Peking University Beijing 100044 ChinaNational Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Peking University People's Hospital Peking University Institute of Hematology Peking University Beijing 100044 ChinaNational Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Peking University People's Hospital Peking University Institute of Hematology Peking University Beijing 100044 ChinaNational Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Peking University People's Hospital Peking University Institute of Hematology Peking University Beijing 100044 ChinaNational Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Peking University People's Hospital Peking University Institute of Hematology Peking University Beijing 100044 ChinaNational Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Peking University People's Hospital Peking University Institute of Hematology Peking University Beijing 100044 ChinaNational Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Peking University People's Hospital Peking University Institute of Hematology Peking University Beijing 100044 ChinaNational Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Peking University People's Hospital Peking University Institute of Hematology Peking University Beijing 100044 ChinaNational Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Peking University People's Hospital Peking University Institute of Hematology Peking University Beijing 100044 ChinaNational Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Peking University People's Hospital Peking University Institute of Hematology Peking University Beijing 100044 ChinaNational Clinical Research Center for Hematologic Disease Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Peking University People's Hospital Peking University Institute of Hematology Peking University Beijing 100044 ChinaAbstract Allogeneic hematopoietic stem cell transplantation (allo‐HSCT) is a widely used treatment for a variety of hematopoietic disorders, and also provides a valuable platform for investigating the development of donor‐derived immune cells in recipients post‐HSCT. The immune system remodels from the donor to the recipient during allo‐HSCT. However, little is known about the cell profile alterations as donor homeostasis rebalances to recipient homeostasis following HSCT. Here, multi‐omics technology is applied at both the single cell and bulk sample levels, as well as spectrum flow cytometry and fluorescent transgenic mouse models, to dissect the dynamics of the rebalanced homeostatic immune system in recipients after allo‐HSCT. The data reveal that all immune subpopulations observed in donors are successfully restored in recipients, though with varying levels of abundance. The remodeling of immune homeostasis exhibits different patterns in HLA‐matched and haploidentical HSCT, highlighting distinct biases in T cell reconstitution from the central and peripheral pathways. Furthermore, ZNF683 is critical for maintaining the persistence and quiescence of CD8 T‐cell in haploidentical HSCT. The research can serve as a foundation for developing novel strategies to induce immune tolerance.https://doi.org/10.1002/advs.202400544allogeneic hematopoietic stem cell transplantationimmune homeostasisimmune reconstitutionimmune toleranceZNF683
spellingShingle Huidong Guo
Liping Guo
Bixia Wang
Xinya Jiang
Zhigui Wu
Xiao‐Dong Mo
Yu‐Qian Sun
Yuan‐Yuan Zhang
Zhi‐Dong Wang
Jun Kong
Chen‐Hua Yan
Xiao‐Jun Huang
Distinct Immune Homeostasis Remodeling Patterns after HLA‐Matched and Haploidentical Transplantation
Advanced Science
allogeneic hematopoietic stem cell transplantation
immune homeostasis
immune reconstitution
immune tolerance
ZNF683
title Distinct Immune Homeostasis Remodeling Patterns after HLA‐Matched and Haploidentical Transplantation
title_full Distinct Immune Homeostasis Remodeling Patterns after HLA‐Matched and Haploidentical Transplantation
title_fullStr Distinct Immune Homeostasis Remodeling Patterns after HLA‐Matched and Haploidentical Transplantation
title_full_unstemmed Distinct Immune Homeostasis Remodeling Patterns after HLA‐Matched and Haploidentical Transplantation
title_short Distinct Immune Homeostasis Remodeling Patterns after HLA‐Matched and Haploidentical Transplantation
title_sort distinct immune homeostasis remodeling patterns after hla matched and haploidentical transplantation
topic allogeneic hematopoietic stem cell transplantation
immune homeostasis
immune reconstitution
immune tolerance
ZNF683
url https://doi.org/10.1002/advs.202400544
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