Molecular detection and phylogenetic analysis of Anaplasma phagocytophilum bacteria in cows and it is infested ticks in Mosul city, Iraq
The present study was conducted to molecular detection of Anaplasma phagocytophilum in infected cows and it is infested ticks in Mosul, Iraq using nested polymerase chain reaction technique (N-PCR) and to investigate the phylogenetic analysis of A. phagocytophilum diagnosed in this study. A total of...
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College of Veterinary Medicine, University of Basrah, Iraq
2023-06-01
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| Series: | Basrah Journal of Veterinary Research |
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| Online Access: | https://bjvr.uobasrah.edu.iq/article_179942_4a976e44d0d43a54edfea6802d169eb0.pdf |
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| author | Abeer Alnakeeb Qaes Al-Obaidi |
| author_facet | Abeer Alnakeeb Qaes Al-Obaidi |
| author_sort | Abeer Alnakeeb |
| collection | DOAJ |
| description | The present study was conducted to molecular detection of Anaplasma phagocytophilum in infected cows and it is infested ticks in Mosul, Iraq using nested polymerase chain reaction technique (N-PCR) and to investigate the phylogenetic analysis of A. phagocytophilum diagnosed in this study. A total of 50 blood samples (3 ml) were collected from cows in various areas of Mosul city. Additionally, 169 hard ticks were collected from different parts of infected cows. Results revealed that the infection rate of A. phagocytophilum in cows at Mosul city was 72% and in engorged female ticks was 81.2% using N-PCR technique. The infestation rate hard ticks on cows were 46% and four species of hard ticks were microscopically identified and classified includes Hyalomma anatolicum anatolicum (39.64%), Rhipicephalus turanicus (21.89%), Rh. sanguineus (23.07%) and Boophillus annulatus (15.38%) with significantly predominant Hyalomma anatolicum anatolicum. The individual sequencing analysis for five sequences of 16S rRNA gene includes: one extracted from cow blood and four extracted from engorgement female ticks. The sequences of A. phagocytophilum were recorded in the NCBI Genbank under the accession numbers (OR002120.1, OR002121.1, OR002122.1, OR002123.1 and OR002124.1). These sequences were highly identity (100%) to those sequences recorded in the NCBI Genbank such as (MT221233.1, MT221234.1) in Norway, (MK239930.1, MK239931.1) in Southern Korea, (MN170722.1, MN170722.1) in Spain, (MK814411.1, MK814412.1) in South Africa, (LC435049.1, LC435050.1) in Japan and (MH122889.1, OQ727069.1) in Poland, and Malawi respectively. This finding might be advantageous for the future studies and strategically control of this bacterium in the study area. |
| format | Article |
| id | doaj-art-2ff77a91824b4e67a81477f3bf9c2fea |
| institution | OA Journals |
| issn | 1813-8497 2410-8456 |
| language | English |
| publishDate | 2023-06-01 |
| publisher | College of Veterinary Medicine, University of Basrah, Iraq |
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| series | Basrah Journal of Veterinary Research |
| spelling | doaj-art-2ff77a91824b4e67a81477f3bf9c2fea2025-08-20T02:30:55ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562023-06-01222334810.23975/bjvetr.2023.179942179942Molecular detection and phylogenetic analysis of Anaplasma phagocytophilum bacteria in cows and it is infested ticks in Mosul city, IraqAbeer Alnakeeb0Qaes Al-Obaidi1Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Internal and Preventive Medicine, College of Veterinary Medicine, University of Mosul, Mosul, IraqThe present study was conducted to molecular detection of Anaplasma phagocytophilum in infected cows and it is infested ticks in Mosul, Iraq using nested polymerase chain reaction technique (N-PCR) and to investigate the phylogenetic analysis of A. phagocytophilum diagnosed in this study. A total of 50 blood samples (3 ml) were collected from cows in various areas of Mosul city. Additionally, 169 hard ticks were collected from different parts of infected cows. Results revealed that the infection rate of A. phagocytophilum in cows at Mosul city was 72% and in engorged female ticks was 81.2% using N-PCR technique. The infestation rate hard ticks on cows were 46% and four species of hard ticks were microscopically identified and classified includes Hyalomma anatolicum anatolicum (39.64%), Rhipicephalus turanicus (21.89%), Rh. sanguineus (23.07%) and Boophillus annulatus (15.38%) with significantly predominant Hyalomma anatolicum anatolicum. The individual sequencing analysis for five sequences of 16S rRNA gene includes: one extracted from cow blood and four extracted from engorgement female ticks. The sequences of A. phagocytophilum were recorded in the NCBI Genbank under the accession numbers (OR002120.1, OR002121.1, OR002122.1, OR002123.1 and OR002124.1). These sequences were highly identity (100%) to those sequences recorded in the NCBI Genbank such as (MT221233.1, MT221234.1) in Norway, (MK239930.1, MK239931.1) in Southern Korea, (MN170722.1, MN170722.1) in Spain, (MK814411.1, MK814412.1) in South Africa, (LC435049.1, LC435050.1) in Japan and (MH122889.1, OQ727069.1) in Poland, and Malawi respectively. This finding might be advantageous for the future studies and strategically control of this bacterium in the study area.https://bjvr.uobasrah.edu.iq/article_179942_4a976e44d0d43a54edfea6802d169eb0.pdfanaplasma phagocytophilumphylogenetic analysismosul |
| spellingShingle | Abeer Alnakeeb Qaes Al-Obaidi Molecular detection and phylogenetic analysis of Anaplasma phagocytophilum bacteria in cows and it is infested ticks in Mosul city, Iraq Basrah Journal of Veterinary Research anaplasma phagocytophilum phylogenetic analysis mosul |
| title | Molecular detection and phylogenetic analysis of Anaplasma phagocytophilum bacteria in cows and it is infested ticks in Mosul city, Iraq |
| title_full | Molecular detection and phylogenetic analysis of Anaplasma phagocytophilum bacteria in cows and it is infested ticks in Mosul city, Iraq |
| title_fullStr | Molecular detection and phylogenetic analysis of Anaplasma phagocytophilum bacteria in cows and it is infested ticks in Mosul city, Iraq |
| title_full_unstemmed | Molecular detection and phylogenetic analysis of Anaplasma phagocytophilum bacteria in cows and it is infested ticks in Mosul city, Iraq |
| title_short | Molecular detection and phylogenetic analysis of Anaplasma phagocytophilum bacteria in cows and it is infested ticks in Mosul city, Iraq |
| title_sort | molecular detection and phylogenetic analysis of anaplasma phagocytophilum bacteria in cows and it is infested ticks in mosul city iraq |
| topic | anaplasma phagocytophilum phylogenetic analysis mosul |
| url | https://bjvr.uobasrah.edu.iq/article_179942_4a976e44d0d43a54edfea6802d169eb0.pdf |
| work_keys_str_mv | AT abeeralnakeeb moleculardetectionandphylogeneticanalysisofanaplasmaphagocytophilumbacteriaincowsanditisinfestedticksinmosulcityiraq AT qaesalobaidi moleculardetectionandphylogeneticanalysisofanaplasmaphagocytophilumbacteriaincowsanditisinfestedticksinmosulcityiraq |