Genome-Wide In Silico Analysis of 1-Aminocyclopropane-1-carboxylate oxidase (ACO) Gene Family in Rice (<i>Oryza sativa</i> L.)
The plant hormone ethylene elicits crucial regulatory effects on plant growth, development, and stress resistance. As the enzyme that catalyzes the final step of ethylene biosynthesis, 1-Aminocyclopropane-1-carboxylic acid oxidase (ACO) plays a key role in precisely controlling ethylene production....
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MDPI AG
2024-12-01
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| author | Jing Xia Yingsheng Qiu Wanli Li Yingcheng Zhang Linxin Liu Yi Wang Wangshu Mou Dawei Xue |
| author_facet | Jing Xia Yingsheng Qiu Wanli Li Yingcheng Zhang Linxin Liu Yi Wang Wangshu Mou Dawei Xue |
| author_sort | Jing Xia |
| collection | DOAJ |
| description | The plant hormone ethylene elicits crucial regulatory effects on plant growth, development, and stress resistance. As the enzyme that catalyzes the final step of ethylene biosynthesis, 1-Aminocyclopropane-1-carboxylic acid oxidase (ACO) plays a key role in precisely controlling ethylene production. However, the functional characterization of the <i>ACO</i> gene family in rice remains largely unexplored. In this study, we performed a phylogenetic analysis of seven <i>OsACO</i> genes (<i>OsACO1</i>–<i>OsACO7</i>), which were classified into three subfamilies (Types I, II, and III). The members within the same clades exhibited similar tertiary structures and conserved protein motifs. We conducted inter/intraspecies covariance assays of OsACOs to elucidate their evolutionary and duplication events. Numerous cis-acting elements identified in <i>OsACO</i> promoter regions are associated with development, hormonal stimuli, and environmental responses. The expression assay by RT-qPCR revealed that <i>OsACO</i> genes exhibited tissue-specific expression and were significantly altered under various abiotic stresses, indicating their potential involvement in these processes regulated at the transcriptional level. Additionally, we predicted candidate-targeting miRNAs and identified putative cysteine sites of S-nitrosylation (SNO) and S-sulfhydration (SSH) in OsACOs, providing insights into their post-transcriptional and post-translational regulatory mechanisms. These findings pave the way for the further investigation of OsACO functions and their potential applications in improving rice growth and stress resilience by modulating ethylene biosynthesis. |
| format | Article |
| id | doaj-art-2ff7200a9a0f487ba1b7f8b9da2e8812 |
| institution | DOAJ |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-2ff7200a9a0f487ba1b7f8b9da2e88122025-08-20T02:56:58ZengMDPI AGPlants2223-77472024-12-011324349010.3390/plants13243490Genome-Wide In Silico Analysis of 1-Aminocyclopropane-1-carboxylate oxidase (ACO) Gene Family in Rice (<i>Oryza sativa</i> L.)Jing Xia0Yingsheng Qiu1Wanli Li2Yingcheng Zhang3Linxin Liu4Yi Wang5Wangshu Mou6Dawei Xue7College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaThe plant hormone ethylene elicits crucial regulatory effects on plant growth, development, and stress resistance. As the enzyme that catalyzes the final step of ethylene biosynthesis, 1-Aminocyclopropane-1-carboxylic acid oxidase (ACO) plays a key role in precisely controlling ethylene production. However, the functional characterization of the <i>ACO</i> gene family in rice remains largely unexplored. In this study, we performed a phylogenetic analysis of seven <i>OsACO</i> genes (<i>OsACO1</i>–<i>OsACO7</i>), which were classified into three subfamilies (Types I, II, and III). The members within the same clades exhibited similar tertiary structures and conserved protein motifs. We conducted inter/intraspecies covariance assays of OsACOs to elucidate their evolutionary and duplication events. Numerous cis-acting elements identified in <i>OsACO</i> promoter regions are associated with development, hormonal stimuli, and environmental responses. The expression assay by RT-qPCR revealed that <i>OsACO</i> genes exhibited tissue-specific expression and were significantly altered under various abiotic stresses, indicating their potential involvement in these processes regulated at the transcriptional level. Additionally, we predicted candidate-targeting miRNAs and identified putative cysteine sites of S-nitrosylation (SNO) and S-sulfhydration (SSH) in OsACOs, providing insights into their post-transcriptional and post-translational regulatory mechanisms. These findings pave the way for the further investigation of OsACO functions and their potential applications in improving rice growth and stress resilience by modulating ethylene biosynthesis.https://www.mdpi.com/2223-7747/13/24/34901-Aminocyclopropane-1-carboxylic acid oxidase (ACO)ethylene biosynthesisricetranscriptional regulationpost-translational modificationabiotic stress |
| spellingShingle | Jing Xia Yingsheng Qiu Wanli Li Yingcheng Zhang Linxin Liu Yi Wang Wangshu Mou Dawei Xue Genome-Wide In Silico Analysis of 1-Aminocyclopropane-1-carboxylate oxidase (ACO) Gene Family in Rice (<i>Oryza sativa</i> L.) Plants 1-Aminocyclopropane-1-carboxylic acid oxidase (ACO) ethylene biosynthesis rice transcriptional regulation post-translational modification abiotic stress |
| title | Genome-Wide In Silico Analysis of 1-Aminocyclopropane-1-carboxylate oxidase (ACO) Gene Family in Rice (<i>Oryza sativa</i> L.) |
| title_full | Genome-Wide In Silico Analysis of 1-Aminocyclopropane-1-carboxylate oxidase (ACO) Gene Family in Rice (<i>Oryza sativa</i> L.) |
| title_fullStr | Genome-Wide In Silico Analysis of 1-Aminocyclopropane-1-carboxylate oxidase (ACO) Gene Family in Rice (<i>Oryza sativa</i> L.) |
| title_full_unstemmed | Genome-Wide In Silico Analysis of 1-Aminocyclopropane-1-carboxylate oxidase (ACO) Gene Family in Rice (<i>Oryza sativa</i> L.) |
| title_short | Genome-Wide In Silico Analysis of 1-Aminocyclopropane-1-carboxylate oxidase (ACO) Gene Family in Rice (<i>Oryza sativa</i> L.) |
| title_sort | genome wide in silico analysis of 1 aminocyclopropane 1 carboxylate oxidase aco gene family in rice i oryza sativa i l |
| topic | 1-Aminocyclopropane-1-carboxylic acid oxidase (ACO) ethylene biosynthesis rice transcriptional regulation post-translational modification abiotic stress |
| url | https://www.mdpi.com/2223-7747/13/24/3490 |
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