Direct Current-Induced Calcium Trafficking in Different Neuronal Preparations
The influence of direct current (DC) stimulation on radioactive calcium trafficking in sciatic nerve in vivo and in vitro, spinal cord, and synaptosomes was investigated. The exposure to DC enhanced calcium redistribution in all of these preparations. The effect was dependent on the strength of the...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2016/2823735 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850217943133061120 |
|---|---|
| author | Andrzej Wieraszko Zaghloul Ahmed |
| author_facet | Andrzej Wieraszko Zaghloul Ahmed |
| author_sort | Andrzej Wieraszko |
| collection | DOAJ |
| description | The influence of direct current (DC) stimulation on radioactive calcium trafficking in sciatic nerve in vivo and in vitro, spinal cord, and synaptosomes was investigated. The exposure to DC enhanced calcium redistribution in all of these preparations. The effect was dependent on the strength of the stimulation and extended beyond the phase of exposure to DC. The DC-induced increase in calcium sequestration by synaptosomes was significantly reduced by cobalt and rupture of synaptosomes by osmotic shock. Although both anodal and cathodal currents were effective, the experiments with two electrodes of different areas revealed that cathodal stimulation exerted stronger effect. The exposure to DC induced not only relocation but also redistribution of calcium within segments of the sciatic nerve. Enzymatic removal of sialic acid by preincubation of synaptosomes with neuroaminidase, or carrying out the experiments in sodium-free environment, amplified DC-induced calcium accumulation. |
| format | Article |
| id | doaj-art-2ff56e3214184cad9609ee3a2f628fd2 |
| institution | OA Journals |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-2ff56e3214184cad9609ee3a2f628fd22025-08-20T02:07:56ZengWileyNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/28237352823735Direct Current-Induced Calcium Trafficking in Different Neuronal PreparationsAndrzej Wieraszko0Zaghloul Ahmed1Department of Biology, The College of Staten Island/City University of New York, 2800 Victory Boulevard, Staten Island, NY 10314, USADepartment of Physical Therapy, The College of Staten Island/City University of New York, 2800 Victory Boulevard, Staten Island, NY 10314, USAThe influence of direct current (DC) stimulation on radioactive calcium trafficking in sciatic nerve in vivo and in vitro, spinal cord, and synaptosomes was investigated. The exposure to DC enhanced calcium redistribution in all of these preparations. The effect was dependent on the strength of the stimulation and extended beyond the phase of exposure to DC. The DC-induced increase in calcium sequestration by synaptosomes was significantly reduced by cobalt and rupture of synaptosomes by osmotic shock. Although both anodal and cathodal currents were effective, the experiments with two electrodes of different areas revealed that cathodal stimulation exerted stronger effect. The exposure to DC induced not only relocation but also redistribution of calcium within segments of the sciatic nerve. Enzymatic removal of sialic acid by preincubation of synaptosomes with neuroaminidase, or carrying out the experiments in sodium-free environment, amplified DC-induced calcium accumulation.http://dx.doi.org/10.1155/2016/2823735 |
| spellingShingle | Andrzej Wieraszko Zaghloul Ahmed Direct Current-Induced Calcium Trafficking in Different Neuronal Preparations Neural Plasticity |
| title | Direct Current-Induced Calcium Trafficking in Different Neuronal Preparations |
| title_full | Direct Current-Induced Calcium Trafficking in Different Neuronal Preparations |
| title_fullStr | Direct Current-Induced Calcium Trafficking in Different Neuronal Preparations |
| title_full_unstemmed | Direct Current-Induced Calcium Trafficking in Different Neuronal Preparations |
| title_short | Direct Current-Induced Calcium Trafficking in Different Neuronal Preparations |
| title_sort | direct current induced calcium trafficking in different neuronal preparations |
| url | http://dx.doi.org/10.1155/2016/2823735 |
| work_keys_str_mv | AT andrzejwieraszko directcurrentinducedcalciumtraffickingindifferentneuronalpreparations AT zaghloulahmed directcurrentinducedcalciumtraffickingindifferentneuronalpreparations |