Fecal Secretory Immunoglobulin A and Lactate Level as a Biomarker of Mucosal Immune Dysfunction in Horses With Colic

ABSTRACT Background Colic‐related obstructions can reduced intestinal mucosa function and cause dysbiosis in horses, but it is unclear how defense barrier and secretory immunoglobulin A (SIgA) secretion is disrupted. Objectives The aim of the study is to evaluate the effect of severity of colic sign...

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Main Authors: Agnieszka Żak‐Bochenek, Zuzana Drábková, Vaiva Sergedaite, Natalia Siwińska, Joanna Bajzert, Dominika Pasak, Anna Chełmońska‐Soyta
Format: Article
Language:English
Published: Wiley 2025-05-01
Series:Journal of Veterinary Internal Medicine
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Online Access:https://doi.org/10.1111/jvim.70073
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author Agnieszka Żak‐Bochenek
Zuzana Drábková
Vaiva Sergedaite
Natalia Siwińska
Joanna Bajzert
Dominika Pasak
Anna Chełmońska‐Soyta
author_facet Agnieszka Żak‐Bochenek
Zuzana Drábková
Vaiva Sergedaite
Natalia Siwińska
Joanna Bajzert
Dominika Pasak
Anna Chełmońska‐Soyta
author_sort Agnieszka Żak‐Bochenek
collection DOAJ
description ABSTRACT Background Colic‐related obstructions can reduced intestinal mucosa function and cause dysbiosis in horses, but it is unclear how defense barrier and secretory immunoglobulin A (SIgA) secretion is disrupted. Objectives The aim of the study is to evaluate the effect of severity of colic signs and treatments on fecal SIgA and fecal lactate in horses. Animals Sixty‐two client owned hospitalised horses with colic and eight healthy horses. Methods Prospective clinical trial. Fecal samples were taken daily for 7 days. SIgA was analyzed using ELISA, and D/L‐lactate measured with a commercial kit. Results At Day 0, SIgA values in the colic medical and colic surgical groups were significantly higher than in the control stable group (U = 126.0, p = 0.099, Cliff's ∆ = 0.58 and U = 248.0, p = 0.005, Cliff's ∆ = 0.72, respectively). We found significant correlation between fecal SIgA and fecal lactate level in D0 (rs = 0.421, p = 0.038). Conclusions This study demonstrates the feasibility of using fecal samples to identify biomarkers of colic in horses. An increase in fecal SIgA in horses with colic might suggest the presence of inflammation within the intestines and disruption of the mucosal barrier. These data highlight changes in gastrointestinal barrier and immune function and the intestinal microbiota's metabolic activity in horses with colic.
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spelling doaj-art-2ff1a7d22c2343c88c307fecad7a24882025-08-20T03:09:11ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762025-05-01393n/an/a10.1111/jvim.70073Fecal Secretory Immunoglobulin A and Lactate Level as a Biomarker of Mucosal Immune Dysfunction in Horses With ColicAgnieszka Żak‐Bochenek0Zuzana Drábková1Vaiva Sergedaite2Natalia Siwińska3Joanna Bajzert4Dominika Pasak5Anna Chełmońska‐Soyta6Department of Immunology, Pathophysiology and Veterinary Preventive Medicine Wroclaw University of Environmental and Life Sciences Wroclaw PolandEquine Clinic, Faculty of Veterinary Medicine University of Veterinary Sciences Brno Brno Czech RepublicEquine Clinic, Faculty of Veterinary Medicine University of Veterinary Sciences Brno Brno Czech RepublicDepartment of Internal Diseases With Clinic of Horses, Dogs and Cats Wroclaw University of Environmental and Life Sciences Wroclaw PolandDepartment of Immunology, Pathophysiology and Veterinary Preventive Medicine Wroclaw University of Environmental and Life Sciences Wroclaw PolandDepartment of Immunology, Pathophysiology and Veterinary Preventive Medicine Wroclaw University of Environmental and Life Sciences Wroclaw PolandDepartment of Immunology, Pathophysiology and Veterinary Preventive Medicine Wroclaw University of Environmental and Life Sciences Wroclaw PolandABSTRACT Background Colic‐related obstructions can reduced intestinal mucosa function and cause dysbiosis in horses, but it is unclear how defense barrier and secretory immunoglobulin A (SIgA) secretion is disrupted. Objectives The aim of the study is to evaluate the effect of severity of colic signs and treatments on fecal SIgA and fecal lactate in horses. Animals Sixty‐two client owned hospitalised horses with colic and eight healthy horses. Methods Prospective clinical trial. Fecal samples were taken daily for 7 days. SIgA was analyzed using ELISA, and D/L‐lactate measured with a commercial kit. Results At Day 0, SIgA values in the colic medical and colic surgical groups were significantly higher than in the control stable group (U = 126.0, p = 0.099, Cliff's ∆ = 0.58 and U = 248.0, p = 0.005, Cliff's ∆ = 0.72, respectively). We found significant correlation between fecal SIgA and fecal lactate level in D0 (rs = 0.421, p = 0.038). Conclusions This study demonstrates the feasibility of using fecal samples to identify biomarkers of colic in horses. An increase in fecal SIgA in horses with colic might suggest the presence of inflammation within the intestines and disruption of the mucosal barrier. These data highlight changes in gastrointestinal barrier and immune function and the intestinal microbiota's metabolic activity in horses with colic.https://doi.org/10.1111/jvim.70073abdomenhorsesintestinelactates
spellingShingle Agnieszka Żak‐Bochenek
Zuzana Drábková
Vaiva Sergedaite
Natalia Siwińska
Joanna Bajzert
Dominika Pasak
Anna Chełmońska‐Soyta
Fecal Secretory Immunoglobulin A and Lactate Level as a Biomarker of Mucosal Immune Dysfunction in Horses With Colic
Journal of Veterinary Internal Medicine
abdomen
horses
intestine
lactates
title Fecal Secretory Immunoglobulin A and Lactate Level as a Biomarker of Mucosal Immune Dysfunction in Horses With Colic
title_full Fecal Secretory Immunoglobulin A and Lactate Level as a Biomarker of Mucosal Immune Dysfunction in Horses With Colic
title_fullStr Fecal Secretory Immunoglobulin A and Lactate Level as a Biomarker of Mucosal Immune Dysfunction in Horses With Colic
title_full_unstemmed Fecal Secretory Immunoglobulin A and Lactate Level as a Biomarker of Mucosal Immune Dysfunction in Horses With Colic
title_short Fecal Secretory Immunoglobulin A and Lactate Level as a Biomarker of Mucosal Immune Dysfunction in Horses With Colic
title_sort fecal secretory immunoglobulin a and lactate level as a biomarker of mucosal immune dysfunction in horses with colic
topic abdomen
horses
intestine
lactates
url https://doi.org/10.1111/jvim.70073
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