Screening of Pesticides with the Potential of Inducing DSB and Successive Recombinational Repair
A study was realized to ascertain whether eight selected pesticides would induce double strand breaks (DSB) in lymphocyte cultures and whether this damage would induce greater levels of proteins Rad51 participating in homologous recombination or of p-Ku80 participating in nonhomologous end joining....
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2017/3574840 |
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| author | Karen Suárez-Larios Ana-María Salazar-Martínez Regina Montero-Montoya |
| author_facet | Karen Suárez-Larios Ana-María Salazar-Martínez Regina Montero-Montoya |
| author_sort | Karen Suárez-Larios |
| collection | DOAJ |
| description | A study was realized to ascertain whether eight selected pesticides would induce double strand breaks (DSB) in lymphocyte cultures and whether this damage would induce greater levels of proteins Rad51 participating in homologous recombination or of p-Ku80 participating in nonhomologous end joining. Only five pesticides were found to induce DSB of which only glyphosate and paraoxon induced a significant increase of p-Ku80 protein, indicating that nonhomologous end joining recombinational DNA repair system would be activated. The type of gamma-H2AX foci observed was comparable to that induced by etoposide at similar concentrations. These results are of importance since these effects occurred at low concentrations in the micromolar range, in acute treatments to the cells. Effects over longer exposures in actual environmental settings are expected to produce cumulative damage if repeated events of recombination take place over time. |
| format | Article |
| id | doaj-art-2fe95b23a4294f64a44e12d97a407797 |
| institution | DOAJ |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-2fe95b23a4294f64a44e12d97a4077972025-08-20T03:20:22ZengWileyJournal of Toxicology1687-81911687-82052017-01-01201710.1155/2017/35748403574840Screening of Pesticides with the Potential of Inducing DSB and Successive Recombinational RepairKaren Suárez-Larios0Ana-María Salazar-Martínez1Regina Montero-Montoya2Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70228, 04510 Ciudad de México, MexicoDepartamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70228, 04510 Ciudad de México, MexicoDepartamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70228, 04510 Ciudad de México, MexicoA study was realized to ascertain whether eight selected pesticides would induce double strand breaks (DSB) in lymphocyte cultures and whether this damage would induce greater levels of proteins Rad51 participating in homologous recombination or of p-Ku80 participating in nonhomologous end joining. Only five pesticides were found to induce DSB of which only glyphosate and paraoxon induced a significant increase of p-Ku80 protein, indicating that nonhomologous end joining recombinational DNA repair system would be activated. The type of gamma-H2AX foci observed was comparable to that induced by etoposide at similar concentrations. These results are of importance since these effects occurred at low concentrations in the micromolar range, in acute treatments to the cells. Effects over longer exposures in actual environmental settings are expected to produce cumulative damage if repeated events of recombination take place over time.http://dx.doi.org/10.1155/2017/3574840 |
| spellingShingle | Karen Suárez-Larios Ana-María Salazar-Martínez Regina Montero-Montoya Screening of Pesticides with the Potential of Inducing DSB and Successive Recombinational Repair Journal of Toxicology |
| title | Screening of Pesticides with the Potential of Inducing DSB and Successive Recombinational Repair |
| title_full | Screening of Pesticides with the Potential of Inducing DSB and Successive Recombinational Repair |
| title_fullStr | Screening of Pesticides with the Potential of Inducing DSB and Successive Recombinational Repair |
| title_full_unstemmed | Screening of Pesticides with the Potential of Inducing DSB and Successive Recombinational Repair |
| title_short | Screening of Pesticides with the Potential of Inducing DSB and Successive Recombinational Repair |
| title_sort | screening of pesticides with the potential of inducing dsb and successive recombinational repair |
| url | http://dx.doi.org/10.1155/2017/3574840 |
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