KAI2-dependent signaling controls vegetative reproduction in Marchantia polymorpha through activation of LOG-mediated cytokinin synthesis (14)
Abstract Marchantia polymorpha reproduces vegetatively (asexually) by producing propagules known as gemmae within gemma cups and sexually through spores. We previously reported that KARRIKIN INSENSITIVE2 (KAI2)-dependent signaling promotes gemma cup and gemma formation. KAI2A perceives unidentified...
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Nature Portfolio
2025-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-55728-3 |
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| author | Aino Komatsu Mizuki Fujibayashi Kazato Kumagai Hidemasa Suzuki Yuki Hata Yumiko Takebayashi Mikiko Kojima Hitoshi Sakakibara Junko Kyozuka |
| author_facet | Aino Komatsu Mizuki Fujibayashi Kazato Kumagai Hidemasa Suzuki Yuki Hata Yumiko Takebayashi Mikiko Kojima Hitoshi Sakakibara Junko Kyozuka |
| author_sort | Aino Komatsu |
| collection | DOAJ |
| description | Abstract Marchantia polymorpha reproduces vegetatively (asexually) by producing propagules known as gemmae within gemma cups and sexually through spores. We previously reported that KARRIKIN INSENSITIVE2 (KAI2)-dependent signaling promotes gemma cup and gemma formation. KAI2A perceives unidentified endogenous ligand(s), tentatively referred to as KAI2 ligands (KL). Perception of KL by KAI2 triggers MORE AXILLARY GROWTH2 (MAX2)-dependent proteolysis of MpSUPPRESSOR of MORE AXILLALRY GROWTH2 1-LIKE (MpSMXL). In this study, we identify genes working downstream of KAI2-dependent signaling in M. polymorpha. We find that KAI2-dependent signaling positively controls the expression of MpLONELY GUY (MpLOG), encoding a cytokinin biosynthesis enzyme. Disruption of the MpLOG function decreases endogenous cytokinin levels and causes defects similar to KAI2-dependent signaling mutants. Moreover, supplying exogenous cytokinins rescues the defects of Mplog and KAI2-dependent signaling mutants, implying that cytokinins work downstream of KAI2-dependent signaling. Activation of MpLOG by KAI2-dependent signaling occurs in a highly cell-type-specific manner, leading to cell-specific induction of GEMMA CUP-ASSOCIATED MYB1 (GCAM1), the master regulator of vegetative reproduction of M. polymorpha. We propose a genetic cascade, starting from KAI2-dependent signaling, that promotes vegetative reproduction through the induction of MpLOG and GCAM1. The interaction between KAI2-dependent signaling and cytokinin in M. polymorpha provides insights into the function and evolution of KAI2-dependent signaling. |
| format | Article |
| id | doaj-art-2fe484950b9c418e8efacee13fe55aa4 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-2fe484950b9c418e8efacee13fe55aa42025-02-02T12:31:48ZengNature PortfolioNature Communications2041-17232025-02-0116111310.1038/s41467-024-55728-3KAI2-dependent signaling controls vegetative reproduction in Marchantia polymorpha through activation of LOG-mediated cytokinin synthesis (14)Aino Komatsu0Mizuki Fujibayashi1Kazato Kumagai2Hidemasa Suzuki3Yuki Hata4Yumiko Takebayashi5Mikiko Kojima6Hitoshi Sakakibara7Junko Kyozuka8Graduate School of Life Sciences, Tohoku UniversityGraduate School of Life Sciences, Tohoku UniversityGraduate School of Life Sciences, Tohoku UniversityGraduate School of Life Sciences, Tohoku UniversityGraduate School of Life Sciences, Tohoku UniversityRIKEN Center for Sustainable Resource ScienceRIKEN Center for Sustainable Resource ScienceRIKEN Center for Sustainable Resource ScienceGraduate School of Life Sciences, Tohoku UniversityAbstract Marchantia polymorpha reproduces vegetatively (asexually) by producing propagules known as gemmae within gemma cups and sexually through spores. We previously reported that KARRIKIN INSENSITIVE2 (KAI2)-dependent signaling promotes gemma cup and gemma formation. KAI2A perceives unidentified endogenous ligand(s), tentatively referred to as KAI2 ligands (KL). Perception of KL by KAI2 triggers MORE AXILLARY GROWTH2 (MAX2)-dependent proteolysis of MpSUPPRESSOR of MORE AXILLALRY GROWTH2 1-LIKE (MpSMXL). In this study, we identify genes working downstream of KAI2-dependent signaling in M. polymorpha. We find that KAI2-dependent signaling positively controls the expression of MpLONELY GUY (MpLOG), encoding a cytokinin biosynthesis enzyme. Disruption of the MpLOG function decreases endogenous cytokinin levels and causes defects similar to KAI2-dependent signaling mutants. Moreover, supplying exogenous cytokinins rescues the defects of Mplog and KAI2-dependent signaling mutants, implying that cytokinins work downstream of KAI2-dependent signaling. Activation of MpLOG by KAI2-dependent signaling occurs in a highly cell-type-specific manner, leading to cell-specific induction of GEMMA CUP-ASSOCIATED MYB1 (GCAM1), the master regulator of vegetative reproduction of M. polymorpha. We propose a genetic cascade, starting from KAI2-dependent signaling, that promotes vegetative reproduction through the induction of MpLOG and GCAM1. The interaction between KAI2-dependent signaling and cytokinin in M. polymorpha provides insights into the function and evolution of KAI2-dependent signaling.https://doi.org/10.1038/s41467-024-55728-3 |
| spellingShingle | Aino Komatsu Mizuki Fujibayashi Kazato Kumagai Hidemasa Suzuki Yuki Hata Yumiko Takebayashi Mikiko Kojima Hitoshi Sakakibara Junko Kyozuka KAI2-dependent signaling controls vegetative reproduction in Marchantia polymorpha through activation of LOG-mediated cytokinin synthesis (14) Nature Communications |
| title | KAI2-dependent signaling controls vegetative reproduction in Marchantia polymorpha through activation of LOG-mediated cytokinin synthesis (14) |
| title_full | KAI2-dependent signaling controls vegetative reproduction in Marchantia polymorpha through activation of LOG-mediated cytokinin synthesis (14) |
| title_fullStr | KAI2-dependent signaling controls vegetative reproduction in Marchantia polymorpha through activation of LOG-mediated cytokinin synthesis (14) |
| title_full_unstemmed | KAI2-dependent signaling controls vegetative reproduction in Marchantia polymorpha through activation of LOG-mediated cytokinin synthesis (14) |
| title_short | KAI2-dependent signaling controls vegetative reproduction in Marchantia polymorpha through activation of LOG-mediated cytokinin synthesis (14) |
| title_sort | kai2 dependent signaling controls vegetative reproduction in marchantia polymorpha through activation of log mediated cytokinin synthesis 14 |
| url | https://doi.org/10.1038/s41467-024-55728-3 |
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