Modified QuEChERS method combined with ultra-high performance liquid chromatography-tandem mass spectrometry for simultaneous determination of 19 quinolones and sulfonamides in aquatic products
<b>Objective:</b> A simultaneous determination method was established for detecting 19 kinds of quinolones and sulfonamides in aquatic products simultaneously, by using QuEChERS combined with UPLC-MS/MS. <b>Methods:</b> Samples were extracted with 1.0% formic acid acetonitril...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Editorial Office of Food and Machinery
2023-10-01
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| Series: | Shipin yu jixie |
| Subjects: | |
| Online Access: | http://www.ifoodmm.com/spyjxen/article/abstract/20230706 |
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| Summary: | <b>Objective:</b> A simultaneous determination method was established for detecting 19 kinds of quinolones and sulfonamides in aquatic products simultaneously, by using QuEChERS combined with UPLC-MS/MS. <b>Methods:</b> Samples were extracted with 1.0% formic acid acetonitrile solution, ultrasonic for 1 min, and purified by QuEChERS multi-function needle filter. The sample purification and filtering were performed simultaneously. The Mass spectrometry was conducted by using positive (ESI+) electrospray ionization and multi-reactions monitoring (MRM) models, with external standard method. <b>Results:</b> The detection limit of 19 kinds of sulfonamides and quinolones is from 0.5 to 1.0 μg/kg. Compared with the national standard method, the detection sensitivity is improved. The added recoveries were from 81.0% to 113.0%, and the relative standard deviation (RSD) were from 0.2% to 11.7%. <b>Conclusion:</b> This method assisted ultrasonic extraction and QuEChERS, enhanced the efficiency of measuring quinolones and sulfonamides in aquatic products. It is a simple, rapid, sensitivity, accurate method, which provides an efficient technical means for daily monitoring of sulfonamides and quinolones in large quantities of aquatic product samples. |
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| ISSN: | 1003-5788 |