The Influence of Sodium Hydrogen Sulfide on Sperm Function and Oxidative Stress in Varicocele-Induced Rats
Objective: This study aims to investigate the effects of sodium hydrogen sulphide (NaHS) supplementation, a hydrogensulphide (H2S) donor, on oxidant and antioxidant markers, as well as sperm function in rats with experimentally inducedvaricocele.Materials and Methods: In this experimental study, 55...
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Royan Institute (ACECR), Tehran
2024-10-01
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| Series: | Cell Journal |
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| Online Access: | https://www.celljournal.org/article_719159_d41d8cd98f00b204e9800998ecf8427e.pdf |
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| author | Elham Ghajari Marziyeh Tavalaee Mohammad Hassan Meshkibaf Maurizio Dattilio Mohammad Hossein Nasr-Esfahani |
| author_facet | Elham Ghajari Marziyeh Tavalaee Mohammad Hassan Meshkibaf Maurizio Dattilio Mohammad Hossein Nasr-Esfahani |
| author_sort | Elham Ghajari |
| collection | DOAJ |
| description | Objective: This study aims to investigate the effects of sodium hydrogen sulphide (NaHS) supplementation, a hydrogensulphide (H2S) donor, on oxidant and antioxidant markers, as well as sperm function in rats with experimentally inducedvaricocele.Materials and Methods: In this experimental study, 55 male Wistar rats were assigned to varicocele (n=25), control (n=20),and sham (n=10) groups. In the varicocele group, five rats received NaHS treatment immediately after surgery for fourmonths and ten rats were treated two to four months after surgery. The remaining ten rats in the varicocele group receivedno treatment. Similar protocols were followed for the control groups. At the end of four months, all rats were sacrificed, andassessments were made for sperm parameters that included function tests, and testicular malondialdehyde (MDA) and totalantioxidant capacity (TAC).Results: Varicocele induction significantly impaired sperm parameters and sperm function tests. NaHS treatment fortwo months increased sperm concentrations, while treatment for two and four months improved motility, chromatinstatus, and intracellular reactive oxygen species (ROS) compared to untreated varicocele rats. After four months, NaHStreatment reduced testicular MDA levels. Testicular TAC significantly increased after two months but decreased afterfour months of treatment in the varicocele group (P<0.05).Conclusion: NaHS treatment improved sperm parameters and reduced oxidative stress in varicocele rats. Theobserved effects depended on the treatment duration. |
| format | Article |
| id | doaj-art-2fe0f8897f8f4c0d8386e1dd5928f989 |
| institution | DOAJ |
| issn | 2228-5806 2228-5814 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj-art-2fe0f8897f8f4c0d8386e1dd5928f9892025-08-20T02:45:37ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142024-10-01261059060110.22074/cellj.2024.2026261.1548719159The Influence of Sodium Hydrogen Sulfide on Sperm Function and Oxidative Stress in Varicocele-Induced RatsElham Ghajari0Marziyeh Tavalaee1Mohammad Hassan Meshkibaf2Maurizio Dattilio3Mohammad Hossein Nasr-Esfahani4Department of Clinical Biochemistry, Faculty of Medicine, Fasa University of Medical Sciences, Fasa, IranDepartment of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, IranDepartment of Clinical Biochemistry, Faculty of Medicine, Fasa University of Medical Sciences, Fasa, IranR&D Department, Parthenogen, Lugano, SwitzerlandDepartment of Animal Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, IranObjective: This study aims to investigate the effects of sodium hydrogen sulphide (NaHS) supplementation, a hydrogensulphide (H2S) donor, on oxidant and antioxidant markers, as well as sperm function in rats with experimentally inducedvaricocele.Materials and Methods: In this experimental study, 55 male Wistar rats were assigned to varicocele (n=25), control (n=20),and sham (n=10) groups. In the varicocele group, five rats received NaHS treatment immediately after surgery for fourmonths and ten rats were treated two to four months after surgery. The remaining ten rats in the varicocele group receivedno treatment. Similar protocols were followed for the control groups. At the end of four months, all rats were sacrificed, andassessments were made for sperm parameters that included function tests, and testicular malondialdehyde (MDA) and totalantioxidant capacity (TAC).Results: Varicocele induction significantly impaired sperm parameters and sperm function tests. NaHS treatment fortwo months increased sperm concentrations, while treatment for two and four months improved motility, chromatinstatus, and intracellular reactive oxygen species (ROS) compared to untreated varicocele rats. After four months, NaHStreatment reduced testicular MDA levels. Testicular TAC significantly increased after two months but decreased afterfour months of treatment in the varicocele group (P<0.05).Conclusion: NaHS treatment improved sperm parameters and reduced oxidative stress in varicocele rats. Theobserved effects depended on the treatment duration.https://www.celljournal.org/article_719159_d41d8cd98f00b204e9800998ecf8427e.pdfchromatinoxidative stresssperm parametersvaricocele |
| spellingShingle | Elham Ghajari Marziyeh Tavalaee Mohammad Hassan Meshkibaf Maurizio Dattilio Mohammad Hossein Nasr-Esfahani The Influence of Sodium Hydrogen Sulfide on Sperm Function and Oxidative Stress in Varicocele-Induced Rats Cell Journal chromatin oxidative stress sperm parameters varicocele |
| title | The Influence of Sodium Hydrogen Sulfide on Sperm Function and Oxidative Stress in Varicocele-Induced Rats |
| title_full | The Influence of Sodium Hydrogen Sulfide on Sperm Function and Oxidative Stress in Varicocele-Induced Rats |
| title_fullStr | The Influence of Sodium Hydrogen Sulfide on Sperm Function and Oxidative Stress in Varicocele-Induced Rats |
| title_full_unstemmed | The Influence of Sodium Hydrogen Sulfide on Sperm Function and Oxidative Stress in Varicocele-Induced Rats |
| title_short | The Influence of Sodium Hydrogen Sulfide on Sperm Function and Oxidative Stress in Varicocele-Induced Rats |
| title_sort | influence of sodium hydrogen sulfide on sperm function and oxidative stress in varicocele induced rats |
| topic | chromatin oxidative stress sperm parameters varicocele |
| url | https://www.celljournal.org/article_719159_d41d8cd98f00b204e9800998ecf8427e.pdf |
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