A Canadian Isolate of Hepatitis D (delta) Virus
HepatitisD(delta) virus (HDV) is an infectious agent that propagates in hepatocytes only in the presence of hepatitis B virus, causing fulminant or chronic hepatitis with liver cirrhosis. HDV is a 36 nm particle that includes a circular RNA genome of 1.7 kilobases with an extensive internal compleme...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2000-01-01
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| Series: | Canadian Journal of Gastroenterology |
| Online Access: | http://dx.doi.org/10.1155/2000/397947 |
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| Summary: | HepatitisD(delta) virus (HDV) is an infectious
agent that propagates in hepatocytes only in the presence
of hepatitis B virus, causing fulminant or chronic hepatitis with
liver cirrhosis. HDV is a 36 nm particle that includes a circular
RNA genome of 1.7 kilobases with an extensive internal complementary
that allows it to fold into a rod-like structure. The relationships
among genotypes, sequence variability, geographical
distribution and disease severity of HDV remain unknown. Consequently,
in the present study, the complete nucleotide sequence
of anHDVisolated from a Canadian patient was determined. The
viral RNA from serum was amplified using reverse transcription
coupled to polymerase chain reaction amplification. The resulting
complementary DNA was cloned and sequenced. Sequence
analysis revealed that this new isolate contained 1672 nucleotides
corresponding to genotype 1, which has a worldwide distribution.
Sequencing of four independent clones revealed 17 substitutions,
corresponding to an overall sequence variability of 1%. Surprisingly,
seven mutations were found in the 48-nucleotide region located
between the two highly conserved self-catalytic motifs. This
is the first demonstration that many substitutions are identified in
this region of HDV, and prompts the present authors to define it
as a hypervariable region. |
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| ISSN: | 0835-7900 |