Study of an arginine- and tryptophan-rich antimicrobial peptide in peri-implantitis
The combination of hydrophilic arginine residues and hydrophobic tryptophan residues is considered to be the first choice for designing short-chain antimicrobial peptides (AMPs) due to their potent antibacterial activity. Based on this, we designed an arginine- and tryptophan-rich short peptide, VR-...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1486213/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841556704477577216 |
|---|---|
| author | Qian Zhang Yalei Jiang Xiaotong He Liwei Liu Xi Zhang |
| author_facet | Qian Zhang Yalei Jiang Xiaotong He Liwei Liu Xi Zhang |
| author_sort | Qian Zhang |
| collection | DOAJ |
| description | The combination of hydrophilic arginine residues and hydrophobic tryptophan residues is considered to be the first choice for designing short-chain antimicrobial peptides (AMPs) due to their potent antibacterial activity. Based on this, we designed an arginine- and tryptophan-rich short peptide, VR-12. Peri-implantitis is a significant microbial inflammatory disorder characterized by the inflammation of the soft tissues surrounding an implant, which ultimately leads to the progressive resorption of the alveolar bone. This study found through antibacterial experiments, wound healing promotion experiments, and anti-inflammatory experiments that VR-12 inhibited and killed planktonic peri-implantitis-associated bacteria, inhibited biofilm formation, and disrupted mature biofilms. Additionally, VR-12 exhibited good biocompatibility with RAW264.7 cells and human gingival fibroblasts (HGFs) cells, promoting proliferation of both cell types. Moreover, VR-12 induced HGFs migration by promoting expression of migration-related factors, thereby promoting soft tissue healing. VR-12 also acted on lipopolysaccharide (LPS)-induced RAW264.7 cells, exerting excellent anti-inflammatory properties by affecting the secretion/expression of inflammation-related factors/genes. Therefore, VR-12 may be a good option for both warding off and treatmenting peri-implantitis. |
| format | Article |
| id | doaj-art-2fd448c8e4b548eebf1cfda28b7c2770 |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-2fd448c8e4b548eebf1cfda28b7c27702025-01-07T06:40:54ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-01-011210.3389/fbioe.2024.14862131486213Study of an arginine- and tryptophan-rich antimicrobial peptide in peri-implantitisQian Zhang0Yalei Jiang1Xiaotong He2Liwei Liu3Xi Zhang4Department of Periodontology, School and Hospital of Stomotology, Tianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, Tianjin Medical University, Tianjin, ChinaDepartment of Periodontology, School and Hospital of Stomotology, Tianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, Tianjin Medical University, Tianjin, ChinaDepartment of Periodontology, School and Hospital of Stomotology, Tianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, Tianjin Medical University, Tianjin, ChinaDepartment of Periodontology, Tianjin Binhai New Area Tanggu Stomatology Hospital, Tianjin, ChinaDepartment of Periodontology, School and Hospital of Stomotology, Tianjin Key Laboratory of Oral Soft and Hard Tissues Restoration and Regeneration, Tianjin Medical University, Tianjin, ChinaThe combination of hydrophilic arginine residues and hydrophobic tryptophan residues is considered to be the first choice for designing short-chain antimicrobial peptides (AMPs) due to their potent antibacterial activity. Based on this, we designed an arginine- and tryptophan-rich short peptide, VR-12. Peri-implantitis is a significant microbial inflammatory disorder characterized by the inflammation of the soft tissues surrounding an implant, which ultimately leads to the progressive resorption of the alveolar bone. This study found through antibacterial experiments, wound healing promotion experiments, and anti-inflammatory experiments that VR-12 inhibited and killed planktonic peri-implantitis-associated bacteria, inhibited biofilm formation, and disrupted mature biofilms. Additionally, VR-12 exhibited good biocompatibility with RAW264.7 cells and human gingival fibroblasts (HGFs) cells, promoting proliferation of both cell types. Moreover, VR-12 induced HGFs migration by promoting expression of migration-related factors, thereby promoting soft tissue healing. VR-12 also acted on lipopolysaccharide (LPS)-induced RAW264.7 cells, exerting excellent anti-inflammatory properties by affecting the secretion/expression of inflammation-related factors/genes. Therefore, VR-12 may be a good option for both warding off and treatmenting peri-implantitis.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1486213/fullperi-implantitisantimicrobial peptidesantibacterial activitywound healinganti-inflammatory activity |
| spellingShingle | Qian Zhang Yalei Jiang Xiaotong He Liwei Liu Xi Zhang Study of an arginine- and tryptophan-rich antimicrobial peptide in peri-implantitis Frontiers in Bioengineering and Biotechnology peri-implantitis antimicrobial peptides antibacterial activity wound healing anti-inflammatory activity |
| title | Study of an arginine- and tryptophan-rich antimicrobial peptide in peri-implantitis |
| title_full | Study of an arginine- and tryptophan-rich antimicrobial peptide in peri-implantitis |
| title_fullStr | Study of an arginine- and tryptophan-rich antimicrobial peptide in peri-implantitis |
| title_full_unstemmed | Study of an arginine- and tryptophan-rich antimicrobial peptide in peri-implantitis |
| title_short | Study of an arginine- and tryptophan-rich antimicrobial peptide in peri-implantitis |
| title_sort | study of an arginine and tryptophan rich antimicrobial peptide in peri implantitis |
| topic | peri-implantitis antimicrobial peptides antibacterial activity wound healing anti-inflammatory activity |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1486213/full |
| work_keys_str_mv | AT qianzhang studyofanarginineandtryptophanrichantimicrobialpeptideinperiimplantitis AT yaleijiang studyofanarginineandtryptophanrichantimicrobialpeptideinperiimplantitis AT xiaotonghe studyofanarginineandtryptophanrichantimicrobialpeptideinperiimplantitis AT liweiliu studyofanarginineandtryptophanrichantimicrobialpeptideinperiimplantitis AT xizhang studyofanarginineandtryptophanrichantimicrobialpeptideinperiimplantitis |