An in vitro study on the inhibition and ultrastructural alterations of Candida albicans biofilm by zinc oxide nanowires in a PMMA matrix
Objectives: The purpose of this study was (i) to investigate whether nanocomposite poly(methyl-methacrylate)-zinc oxide nanowires (PMMA-ZnO-NWs) have C. albicans antibiofilm activity; (ii) to evaluate the interaction between components of the nanocomposites based on PMMA-ZnO-NWs by Raman spectroscop...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2021-12-01
|
| Series: | Saudi Dental Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905221000870 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850280529501356032 |
|---|---|
| author | Constanza Apip Alejandra Martínez Manuel Meléndrez Mariana Domínguez Teresita Marzialetti Ricardo Báez Gabriela Sánchez-Sanhueza Andrés Jaramillo Alfonso Catalán |
| author_facet | Constanza Apip Alejandra Martínez Manuel Meléndrez Mariana Domínguez Teresita Marzialetti Ricardo Báez Gabriela Sánchez-Sanhueza Andrés Jaramillo Alfonso Catalán |
| author_sort | Constanza Apip |
| collection | DOAJ |
| description | Objectives: The purpose of this study was (i) to investigate whether nanocomposite poly(methyl-methacrylate)-zinc oxide nanowires (PMMA-ZnO-NWs) have C. albicans antibiofilm activity; (ii) to evaluate the interaction between components of the nanocomposites based on PMMA-ZnO-NWs by Raman spectroscopy; and (iii) to assess ultrastructural alterations. Design: Sixty-eight rectangles (17 PMMA (control) and 51 PMMA–ZnO-NWs (250, 500, 1000 ppm ZnO nanowires) were fabricated. C. albicans ATCC 10231 and a C. albicans clinical strain were tested. Adherence, biofilm formation and ultrastructural alterations were assessed by transmission electron microscopy. Raman mapping images and spectra were analyzed using main component analysis. Results: Nanocomposite PMMA-ZnO-NWs inhibited the formation of C. albicans biofilms 94% at 1000 ppm and 80% at 500 ppm against both C. albicans strains. PMMA-ZnO-NWs induced ultrastructural alterations, including cell wall damage and disorganization of the cytoplasmic membrane, resulting in cell lysis. Raman spectroscopy showed new vibrational modes (300–365–485–600 cm−1) for PMMA and ZnO-NW interactions. Conclusions: PMMA-ZnO-NWs inhibited C. albicans dose-dependent biofilm formation and led to changes in the structures and cell membrane. Raman spectroscopy showed chemical interactions between ZnO-NWs and PMMA, as suggested by the appearance of new bands at 301 and 485 cm−1. |
| format | Article |
| id | doaj-art-2fd0b9cc9d094471a0f62eda96bf04b9 |
| institution | OA Journals |
| issn | 1013-9052 |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Springer |
| record_format | Article |
| series | Saudi Dental Journal |
| spelling | doaj-art-2fd0b9cc9d094471a0f62eda96bf04b92025-08-20T01:48:41ZengSpringerSaudi Dental Journal1013-90522021-12-0133894495310.1016/j.sdentj.2021.08.006An in vitro study on the inhibition and ultrastructural alterations of Candida albicans biofilm by zinc oxide nanowires in a PMMA matrixConstanza Apip0Alejandra Martínez1Manuel Meléndrez2Mariana Domínguez3Teresita Marzialetti4Ricardo Báez5Gabriela Sánchez-Sanhueza6Andrés Jaramillo7Alfonso Catalán8Oral Prosthetic Rehabilitation Program, Department of Restorative, School of Dentistry, University of Concepción, 1550 Roosevelt, Concepción 4070409, ChileOral Prosthetic Rehabilitation Program, Department of Restorative, School of Dentistry, University of Concepción, 1550 Roosevelt, Concepción 4070409, ChileHybrid Material and Polymer Lab, Department of Materials Engineering, Faculty of Engineering, University of Concepción, Concepción, ChileDepartment of Microbiology, Faculty of Biological Science, University of Concepción, Casilla 160C, Concepción 4070409, ChileDepartment of Chemical Engineering, Faculty of Engineering, University of Concepción, 219 Edmundo Larenas, Concepción 4070409, Chile; Corresponding author.Department of Physics, Faculty of Physical and Mathematical Sciences, University of Concepción, 219 Edmundo Larenas, Concepción 4070409, ChileDepartment of Restorative Dentistry, School of Dentistry, University of Concepción, 1550 Roosevelt St, Concepción 4070409, ChileDepartment of Mechanical Engineering, Universidad de La Frontera, 01145 Francisco Salazar, Temuco 4780000, ChileRemovable Prosthetics, Department of Restorative Dentistry, School of Dentistry, University of Concepción, 1550 Roosevelt St, Concepción 4070409, ChileObjectives: The purpose of this study was (i) to investigate whether nanocomposite poly(methyl-methacrylate)-zinc oxide nanowires (PMMA-ZnO-NWs) have C. albicans antibiofilm activity; (ii) to evaluate the interaction between components of the nanocomposites based on PMMA-ZnO-NWs by Raman spectroscopy; and (iii) to assess ultrastructural alterations. Design: Sixty-eight rectangles (17 PMMA (control) and 51 PMMA–ZnO-NWs (250, 500, 1000 ppm ZnO nanowires) were fabricated. C. albicans ATCC 10231 and a C. albicans clinical strain were tested. Adherence, biofilm formation and ultrastructural alterations were assessed by transmission electron microscopy. Raman mapping images and spectra were analyzed using main component analysis. Results: Nanocomposite PMMA-ZnO-NWs inhibited the formation of C. albicans biofilms 94% at 1000 ppm and 80% at 500 ppm against both C. albicans strains. PMMA-ZnO-NWs induced ultrastructural alterations, including cell wall damage and disorganization of the cytoplasmic membrane, resulting in cell lysis. Raman spectroscopy showed new vibrational modes (300–365–485–600 cm−1) for PMMA and ZnO-NW interactions. Conclusions: PMMA-ZnO-NWs inhibited C. albicans dose-dependent biofilm formation and led to changes in the structures and cell membrane. Raman spectroscopy showed chemical interactions between ZnO-NWs and PMMA, as suggested by the appearance of new bands at 301 and 485 cm−1.http://www.sciencedirect.com/science/article/pii/S1013905221000870C. albicansPoly(methyl-methacrylate)NanowiresZinc oxideBiofilmUltrastructural alteration |
| spellingShingle | Constanza Apip Alejandra Martínez Manuel Meléndrez Mariana Domínguez Teresita Marzialetti Ricardo Báez Gabriela Sánchez-Sanhueza Andrés Jaramillo Alfonso Catalán An in vitro study on the inhibition and ultrastructural alterations of Candida albicans biofilm by zinc oxide nanowires in a PMMA matrix Saudi Dental Journal C. albicans Poly(methyl-methacrylate) Nanowires Zinc oxide Biofilm Ultrastructural alteration |
| title | An in vitro study on the inhibition and ultrastructural alterations of Candida albicans biofilm by zinc oxide nanowires in a PMMA matrix |
| title_full | An in vitro study on the inhibition and ultrastructural alterations of Candida albicans biofilm by zinc oxide nanowires in a PMMA matrix |
| title_fullStr | An in vitro study on the inhibition and ultrastructural alterations of Candida albicans biofilm by zinc oxide nanowires in a PMMA matrix |
| title_full_unstemmed | An in vitro study on the inhibition and ultrastructural alterations of Candida albicans biofilm by zinc oxide nanowires in a PMMA matrix |
| title_short | An in vitro study on the inhibition and ultrastructural alterations of Candida albicans biofilm by zinc oxide nanowires in a PMMA matrix |
| title_sort | in vitro study on the inhibition and ultrastructural alterations of candida albicans biofilm by zinc oxide nanowires in a pmma matrix |
| topic | C. albicans Poly(methyl-methacrylate) Nanowires Zinc oxide Biofilm Ultrastructural alteration |
| url | http://www.sciencedirect.com/science/article/pii/S1013905221000870 |
| work_keys_str_mv | AT constanzaapip aninvitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT alejandramartinez aninvitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT manuelmelendrez aninvitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT marianadominguez aninvitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT teresitamarzialetti aninvitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT ricardobaez aninvitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT gabrielasanchezsanhueza aninvitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT andresjaramillo aninvitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT alfonsocatalan aninvitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT constanzaapip invitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT alejandramartinez invitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT manuelmelendrez invitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT marianadominguez invitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT teresitamarzialetti invitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT ricardobaez invitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT gabrielasanchezsanhueza invitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT andresjaramillo invitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix AT alfonsocatalan invitrostudyontheinhibitionandultrastructuralalterationsofcandidaalbicansbiofilmbyzincoxidenanowiresinapmmamatrix |