Insertion/deletion polymorphism of the human leukocyte antigen G gene in pregnant women with threatened abortion
Introduction: The polymorphism of the human leukocyte antigen G gene is investigated in different complications related to pregnancy; however, it must be verified if there is an association between this genetic polymorphism and the threat of abortion. Objective: To establish the genetic association...
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Universidad de Ciencias Médicas de La Habana
2024-07-01
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| Series: | Revista Habanera de Ciencias Médicas |
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| Online Access: | https://revhabanera.sld.cu/index.php/rhab/article/view/5586 |
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| author | Gerardo Brunet Bernal Georgina Espinosa López Isabel Fabiola Pazos Santos Amilcar Arenal Cruz Grethel Camejo Sampedro Rogelio Puebla Alonso |
| author_facet | Gerardo Brunet Bernal Georgina Espinosa López Isabel Fabiola Pazos Santos Amilcar Arenal Cruz Grethel Camejo Sampedro Rogelio Puebla Alonso |
| author_sort | Gerardo Brunet Bernal |
| collection | DOAJ |
| description | Introduction: The polymorphism of the human leukocyte antigen G gene is investigated in different complications related to pregnancy; however, it must be verified if there is an association between this genetic polymorphism and the threat of abortion.
Objective: To establish the genetic association between the 14 base pair insertion/deletion polymorphism in exon 8 of the three non-translatable prime region of the human leukocyte antigen G gene with susceptibility to the threat of abortion in pregnant women from Camagüey.
Material and Methods: A case-control study was carried out, involving 22 pregnant women with threatened abortion and 65 pregnant women (control group). For the study, human deoxyribonucleic acid was obtained from peripheral blood, the polymerase chain reaction was used for the molecular typing of the genotypes and alleles that were identified by agarose gel electrophoresis.
Results: The most frequent genotype in the group of pregnant women with threatened abortion was insertion/deletion, while in the control group the deletion/deletion genotype predominated.
Conclusions: The insertion/deletion polymorphism of 14 base pairs in exon 8 of the 3’ non-translatable region of the human leukocyte antigen G gene represents a risk factor for the appearance of threatened abortion in pregnant women from Camagüey, while the expression of the deletion/deletion genotype represents a protective factor against this health condition. |
| format | Article |
| id | doaj-art-2fcfd085de674cfd944c1d17d7dffd77 |
| institution | Kabale University |
| issn | 1729-519X |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Universidad de Ciencias Médicas de La Habana |
| record_format | Article |
| series | Revista Habanera de Ciencias Médicas |
| spelling | doaj-art-2fcfd085de674cfd944c1d17d7dffd772025-08-20T03:26:10ZengUniversidad de Ciencias Médicas de La HabanaRevista Habanera de Ciencias Médicas1729-519X2024-07-01230e5586e55862179Insertion/deletion polymorphism of the human leukocyte antigen G gene in pregnant women with threatened abortionGerardo Brunet Bernal0Georgina Espinosa López1Isabel Fabiola Pazos Santos2Amilcar Arenal Cruz3Grethel Camejo Sampedro4Rogelio Puebla Alonso5Centro de Inmunología y Productos Biológicos. Camagüey. Universidad de Ciencias Médicas de Camagüey. Camagüey.Universidad de la Habana, Facultad de Biología, Departamento de Bioquímica. La Habana.Universidad de la Habana, Facultad de Biología, Departamento de Bioquímica. La Habana.Universidad de la Camagüey, Facultad de Ciencias Agropecuarias, Departamento de Biología Molecular. Camagüey.Universidad de Ciencias Médicas de Camagüey. Camagüey. Hospital Municipal de Florida “Manuel Fajardo Rivero”. Camagüey.Universidad de la Habana, Facultad de Biología, Departamento de Bioquímica. La Habana.Introduction: The polymorphism of the human leukocyte antigen G gene is investigated in different complications related to pregnancy; however, it must be verified if there is an association between this genetic polymorphism and the threat of abortion. Objective: To establish the genetic association between the 14 base pair insertion/deletion polymorphism in exon 8 of the three non-translatable prime region of the human leukocyte antigen G gene with susceptibility to the threat of abortion in pregnant women from Camagüey. Material and Methods: A case-control study was carried out, involving 22 pregnant women with threatened abortion and 65 pregnant women (control group). For the study, human deoxyribonucleic acid was obtained from peripheral blood, the polymerase chain reaction was used for the molecular typing of the genotypes and alleles that were identified by agarose gel electrophoresis. Results: The most frequent genotype in the group of pregnant women with threatened abortion was insertion/deletion, while in the control group the deletion/deletion genotype predominated. Conclusions: The insertion/deletion polymorphism of 14 base pairs in exon 8 of the 3’ non-translatable region of the human leukocyte antigen G gene represents a risk factor for the appearance of threatened abortion in pregnant women from Camagüey, while the expression of the deletion/deletion genotype represents a protective factor against this health condition.https://revhabanera.sld.cu/index.php/rhab/article/view/5586amenaza de aborto, polimorfismo genético, antígenos leucocíticos humanos. |
| spellingShingle | Gerardo Brunet Bernal Georgina Espinosa López Isabel Fabiola Pazos Santos Amilcar Arenal Cruz Grethel Camejo Sampedro Rogelio Puebla Alonso Insertion/deletion polymorphism of the human leukocyte antigen G gene in pregnant women with threatened abortion Revista Habanera de Ciencias Médicas amenaza de aborto, polimorfismo genético, antígenos leucocíticos humanos. |
| title | Insertion/deletion polymorphism of the human leukocyte antigen G gene in pregnant women with threatened abortion |
| title_full | Insertion/deletion polymorphism of the human leukocyte antigen G gene in pregnant women with threatened abortion |
| title_fullStr | Insertion/deletion polymorphism of the human leukocyte antigen G gene in pregnant women with threatened abortion |
| title_full_unstemmed | Insertion/deletion polymorphism of the human leukocyte antigen G gene in pregnant women with threatened abortion |
| title_short | Insertion/deletion polymorphism of the human leukocyte antigen G gene in pregnant women with threatened abortion |
| title_sort | insertion deletion polymorphism of the human leukocyte antigen g gene in pregnant women with threatened abortion |
| topic | amenaza de aborto, polimorfismo genético, antígenos leucocíticos humanos. |
| url | https://revhabanera.sld.cu/index.php/rhab/article/view/5586 |
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