Validation of Prognostic Circulating Cell-Free RNA Biomarkers <i>HPGD</i>, <i>PACS1</i>, and <i>TDP2</i> in Colorectal Cancer Through TaqMan qPCR and Correlation Analysis
Circulating cell-free RNAs (cfRNAs) have emerged as promising non-invasive biomarkers for colorectal cancer (CRC), offering insights into the disease’s prognosis. This study investigates the prognostic significance of the specific cfRNA biomarkers <i>HPGD</i>, <i>PACS1</i>, a...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/47/7/508 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849246618111442944 |
|---|---|
| author | Chau Ming Kan Xiao Meng Pei Simon Siu Man Ng Wing Wa Leung Yee Ni Wong Hennie Yuk-Lin Cheng William Chi Shing Cho Hin Fung Tsang Sze Chuen Cesar Wong |
| author_facet | Chau Ming Kan Xiao Meng Pei Simon Siu Man Ng Wing Wa Leung Yee Ni Wong Hennie Yuk-Lin Cheng William Chi Shing Cho Hin Fung Tsang Sze Chuen Cesar Wong |
| author_sort | Chau Ming Kan |
| collection | DOAJ |
| description | Circulating cell-free RNAs (cfRNAs) have emerged as promising non-invasive biomarkers for colorectal cancer (CRC), offering insights into the disease’s prognosis. This study investigates the prognostic significance of the specific cfRNA biomarkers <i>HPGD</i>, <i>PACS1</i>, and <i>TDP2</i> by employing the Taqman quantitative PCR (qPCR) to evaluate their expression levels in a cohort of 52 CRC patients. The methodology involved a robust statistical analysis to assess correlations between cfRNA levels and clinical parameters, including survival rates and recurrence incidences. Findings revealed a significant upregulation in the expression of <i>HPGD</i> and <i>PACS1</i>, while <i>TDP2</i> displayed varying results, indicating a complex role in disease dynamics. Notably, lower expression levels of <i>HPGD</i> were associated with reduced survival, suggesting its potential as a negative prognostic indicator. Conversely, <i>TDP2</i> levels correlated strongly with increased risks of recurrence, highlighting its clinical relevance in monitoring disease progression. Overall, this study elucidates the intricate interplay between these cfRNAs in the CRC prognosis. The results advocate for further exploratory studies to validate <i>PACS1</i>’s potential as a prognostic marker and reinforce the clinical significance of <i>HPGD</i> and <i>TDP2</i> in the context of CRC management, positioning them as vital elements in the landscape of molecular oncology. |
| format | Article |
| id | doaj-art-2fcd8ac09d0e4efebb98257cb3c101e4 |
| institution | Kabale University |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj-art-2fcd8ac09d0e4efebb98257cb3c101e42025-08-20T03:58:26ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-07-0147750810.3390/cimb47070508Validation of Prognostic Circulating Cell-Free RNA Biomarkers <i>HPGD</i>, <i>PACS1</i>, and <i>TDP2</i> in Colorectal Cancer Through TaqMan qPCR and Correlation AnalysisChau Ming Kan0Xiao Meng Pei1Simon Siu Man Ng2Wing Wa Leung3Yee Ni Wong4Hennie Yuk-Lin Cheng5William Chi Shing Cho6Hin Fung Tsang7Sze Chuen Cesar Wong8Department of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, Hong Kong SAR, ChinaDepartment of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, Hong Kong SAR, ChinaDepartment of Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, ChinaDepartment of Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, ChinaDepartment of Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SAR, ChinaDepartment of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, Hong Kong SAR, ChinaDepartment of Clinical Oncology, Queen Elizabeth Hospital, Kowloon, Hong Kong SAR, ChinaDepartment of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, Hong Kong SAR, ChinaDepartment of Applied Biology & Chemical Technology, The Hong Kong Polytechnic University, Hong Kong SAR, ChinaCirculating cell-free RNAs (cfRNAs) have emerged as promising non-invasive biomarkers for colorectal cancer (CRC), offering insights into the disease’s prognosis. This study investigates the prognostic significance of the specific cfRNA biomarkers <i>HPGD</i>, <i>PACS1</i>, and <i>TDP2</i> by employing the Taqman quantitative PCR (qPCR) to evaluate their expression levels in a cohort of 52 CRC patients. The methodology involved a robust statistical analysis to assess correlations between cfRNA levels and clinical parameters, including survival rates and recurrence incidences. Findings revealed a significant upregulation in the expression of <i>HPGD</i> and <i>PACS1</i>, while <i>TDP2</i> displayed varying results, indicating a complex role in disease dynamics. Notably, lower expression levels of <i>HPGD</i> were associated with reduced survival, suggesting its potential as a negative prognostic indicator. Conversely, <i>TDP2</i> levels correlated strongly with increased risks of recurrence, highlighting its clinical relevance in monitoring disease progression. Overall, this study elucidates the intricate interplay between these cfRNAs in the CRC prognosis. The results advocate for further exploratory studies to validate <i>PACS1</i>’s potential as a prognostic marker and reinforce the clinical significance of <i>HPGD</i> and <i>TDP2</i> in the context of CRC management, positioning them as vital elements in the landscape of molecular oncology.https://www.mdpi.com/1467-3045/47/7/508colorectal cancer (CRC)Taqman quantitative PCR (qPCR)circulating cell-free RNAs (cfRNAs)<i>HPGD</i><i>TDP2</i><i>PACS1</i> |
| spellingShingle | Chau Ming Kan Xiao Meng Pei Simon Siu Man Ng Wing Wa Leung Yee Ni Wong Hennie Yuk-Lin Cheng William Chi Shing Cho Hin Fung Tsang Sze Chuen Cesar Wong Validation of Prognostic Circulating Cell-Free RNA Biomarkers <i>HPGD</i>, <i>PACS1</i>, and <i>TDP2</i> in Colorectal Cancer Through TaqMan qPCR and Correlation Analysis Current Issues in Molecular Biology colorectal cancer (CRC) Taqman quantitative PCR (qPCR) circulating cell-free RNAs (cfRNAs) <i>HPGD</i> <i>TDP2</i> <i>PACS1</i> |
| title | Validation of Prognostic Circulating Cell-Free RNA Biomarkers <i>HPGD</i>, <i>PACS1</i>, and <i>TDP2</i> in Colorectal Cancer Through TaqMan qPCR and Correlation Analysis |
| title_full | Validation of Prognostic Circulating Cell-Free RNA Biomarkers <i>HPGD</i>, <i>PACS1</i>, and <i>TDP2</i> in Colorectal Cancer Through TaqMan qPCR and Correlation Analysis |
| title_fullStr | Validation of Prognostic Circulating Cell-Free RNA Biomarkers <i>HPGD</i>, <i>PACS1</i>, and <i>TDP2</i> in Colorectal Cancer Through TaqMan qPCR and Correlation Analysis |
| title_full_unstemmed | Validation of Prognostic Circulating Cell-Free RNA Biomarkers <i>HPGD</i>, <i>PACS1</i>, and <i>TDP2</i> in Colorectal Cancer Through TaqMan qPCR and Correlation Analysis |
| title_short | Validation of Prognostic Circulating Cell-Free RNA Biomarkers <i>HPGD</i>, <i>PACS1</i>, and <i>TDP2</i> in Colorectal Cancer Through TaqMan qPCR and Correlation Analysis |
| title_sort | validation of prognostic circulating cell free rna biomarkers i hpgd i i pacs1 i and i tdp2 i in colorectal cancer through taqman qpcr and correlation analysis |
| topic | colorectal cancer (CRC) Taqman quantitative PCR (qPCR) circulating cell-free RNAs (cfRNAs) <i>HPGD</i> <i>TDP2</i> <i>PACS1</i> |
| url | https://www.mdpi.com/1467-3045/47/7/508 |
| work_keys_str_mv | AT chaumingkan validationofprognosticcirculatingcellfreernabiomarkersihpgdiipacs1ianditdp2iincolorectalcancerthroughtaqmanqpcrandcorrelationanalysis AT xiaomengpei validationofprognosticcirculatingcellfreernabiomarkersihpgdiipacs1ianditdp2iincolorectalcancerthroughtaqmanqpcrandcorrelationanalysis AT simonsiumanng validationofprognosticcirculatingcellfreernabiomarkersihpgdiipacs1ianditdp2iincolorectalcancerthroughtaqmanqpcrandcorrelationanalysis AT wingwaleung validationofprognosticcirculatingcellfreernabiomarkersihpgdiipacs1ianditdp2iincolorectalcancerthroughtaqmanqpcrandcorrelationanalysis AT yeeniwong validationofprognosticcirculatingcellfreernabiomarkersihpgdiipacs1ianditdp2iincolorectalcancerthroughtaqmanqpcrandcorrelationanalysis AT hennieyuklincheng validationofprognosticcirculatingcellfreernabiomarkersihpgdiipacs1ianditdp2iincolorectalcancerthroughtaqmanqpcrandcorrelationanalysis AT williamchishingcho validationofprognosticcirculatingcellfreernabiomarkersihpgdiipacs1ianditdp2iincolorectalcancerthroughtaqmanqpcrandcorrelationanalysis AT hinfungtsang validationofprognosticcirculatingcellfreernabiomarkersihpgdiipacs1ianditdp2iincolorectalcancerthroughtaqmanqpcrandcorrelationanalysis AT szechuencesarwong validationofprognosticcirculatingcellfreernabiomarkersihpgdiipacs1ianditdp2iincolorectalcancerthroughtaqmanqpcrandcorrelationanalysis |