A pan-genotypic indirect competitive ELISA for serological detection of pigeon circovirus antibodies
Pigeon circovirus (PiCV) infection, which causes young pigeon disease syndrome (YPDS) and immunosuppression, significantly impacts both the meat and racing pigeon industries. Currently, no inactivated vaccine exists for PiCV prevention, primarily due to the challenges associated with isolating the P...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1612715/full |
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| author | Weifan Wang Weifan Wang Sa Xiao Man Zhang Jinming Liu Jianxia Tian Chuanyu Chang Yuzhen Li Yajie Zhang Fuliang Zhang Fuliang Zhang Guiming Li Guiming Li Xiaoyuan Yuan Xiaoyuan Yuan Wenbin Wang Wenbin Wang |
| author_facet | Weifan Wang Weifan Wang Sa Xiao Man Zhang Jinming Liu Jianxia Tian Chuanyu Chang Yuzhen Li Yajie Zhang Fuliang Zhang Fuliang Zhang Guiming Li Guiming Li Xiaoyuan Yuan Xiaoyuan Yuan Wenbin Wang Wenbin Wang |
| author_sort | Weifan Wang |
| collection | DOAJ |
| description | Pigeon circovirus (PiCV) infection, which causes young pigeon disease syndrome (YPDS) and immunosuppression, significantly impacts both the meat and racing pigeon industries. Currently, no inactivated vaccine exists for PiCV prevention, primarily due to the challenges associated with isolating the PiCV virion, except for some gene subunit vaccines express the Cap protein of PiCV. The development of detection techniques is crucial for the diagnosis of PiCV. This study aimed to develop and validate a specific, sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA) for detecting PiCV antibodies in pigeons. We identified the cap gene from a group C PiCV strain (PiCV/Shaanxi/China/10/2021, SX10) isolated from racing pigeons. The Cap of SX10, an immunogen, can self-assemble into virus-like particles (VLPs). A mouse monoclonal antibody (mAb) against Cap, 1G6-4C4, was selected to establish an icELISA. This mAb could identify the PiCV Cap of the strains in groups A to E. The pan-genotypic reactivity of mAb 1G6-4C4 might target a conserved conformational epitope, overcoming limitations of PCR and prior serological assays. The icELISA method exhibited no cross-reactivity with antibodies against other common pigeon pathogens, such as pigeon paramyxovirus type 1 (PPMV-1), avian influenza (H9N2), avian adenovirus type 4 (FAdV-4) or rotavirus (RV). Compared with indirect ELISA (iELISA), icELISA demonstrated comparable performance, as testing of 29 clinical serum samples revealed antibody-positive rates of 51.72% (icELISA) and 44.82% (iELISA), with a 93.10% concordance rate. To a certain extent, icELISA has demonstrated good specificity and sensitivity for detecting PiCV-specific antibodies in pigeons. The developed icELISA provides a robust, specific, and sensitive tool for the serological detection of PiCV infection. Complementary to PCR test, icELISA enhances the comprehensive detection of PICV in epidemiological studies by offering a more practical and sensitive alternative for field applications. Its utility for large-scale epidemiological surveillance in PiCV-endemic regions is validated, highlighting its potential to inform targeted biosecurity and control interventions. |
| format | Article |
| id | doaj-art-2fcd351b0d6c4b47bc167f1a9b65b821 |
| institution | DOAJ |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-2fcd351b0d6c4b47bc167f1a9b65b8212025-08-20T02:46:17ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.16127151612715A pan-genotypic indirect competitive ELISA for serological detection of pigeon circovirus antibodiesWeifan Wang0Weifan Wang1Sa Xiao2Man Zhang3Jinming Liu4Jianxia Tian5Chuanyu Chang6Yuzhen Li7Yajie Zhang8Fuliang Zhang9Fuliang Zhang10Guiming Li11Guiming Li12Xiaoyuan Yuan13Xiaoyuan Yuan14Wenbin Wang15Wenbin Wang16Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, ChinaYangling Vocation and Technical College, Yangling, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling, ChinaPoultry Institute, Shandong Academy of Agricultural Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Livestock and Poultry Breeding (PKL2024B15), Jinan, Shandong, ChinaPoultry Institute, Shandong Academy of Agricultural Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Livestock and Poultry Breeding (PKL2024B15), Jinan, Shandong, ChinaPoultry Institute, Shandong Academy of Agricultural Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Livestock and Poultry Breeding (PKL2024B15), Jinan, Shandong, ChinaPoultry Institute, Shandong Academy of Agricultural Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Livestock and Poultry Breeding (PKL2024B15), Jinan, Shandong, ChinaPigeon circovirus (PiCV) infection, which causes young pigeon disease syndrome (YPDS) and immunosuppression, significantly impacts both the meat and racing pigeon industries. Currently, no inactivated vaccine exists for PiCV prevention, primarily due to the challenges associated with isolating the PiCV virion, except for some gene subunit vaccines express the Cap protein of PiCV. The development of detection techniques is crucial for the diagnosis of PiCV. This study aimed to develop and validate a specific, sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA) for detecting PiCV antibodies in pigeons. We identified the cap gene from a group C PiCV strain (PiCV/Shaanxi/China/10/2021, SX10) isolated from racing pigeons. The Cap of SX10, an immunogen, can self-assemble into virus-like particles (VLPs). A mouse monoclonal antibody (mAb) against Cap, 1G6-4C4, was selected to establish an icELISA. This mAb could identify the PiCV Cap of the strains in groups A to E. The pan-genotypic reactivity of mAb 1G6-4C4 might target a conserved conformational epitope, overcoming limitations of PCR and prior serological assays. The icELISA method exhibited no cross-reactivity with antibodies against other common pigeon pathogens, such as pigeon paramyxovirus type 1 (PPMV-1), avian influenza (H9N2), avian adenovirus type 4 (FAdV-4) or rotavirus (RV). Compared with indirect ELISA (iELISA), icELISA demonstrated comparable performance, as testing of 29 clinical serum samples revealed antibody-positive rates of 51.72% (icELISA) and 44.82% (iELISA), with a 93.10% concordance rate. To a certain extent, icELISA has demonstrated good specificity and sensitivity for detecting PiCV-specific antibodies in pigeons. The developed icELISA provides a robust, specific, and sensitive tool for the serological detection of PiCV infection. Complementary to PCR test, icELISA enhances the comprehensive detection of PICV in epidemiological studies by offering a more practical and sensitive alternative for field applications. Its utility for large-scale epidemiological surveillance in PiCV-endemic regions is validated, highlighting its potential to inform targeted biosecurity and control interventions.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1612715/fullindirect competitive ELISApigeon circovirusCapvirus-like particlesmonoclonal antibody |
| spellingShingle | Weifan Wang Weifan Wang Sa Xiao Man Zhang Jinming Liu Jianxia Tian Chuanyu Chang Yuzhen Li Yajie Zhang Fuliang Zhang Fuliang Zhang Guiming Li Guiming Li Xiaoyuan Yuan Xiaoyuan Yuan Wenbin Wang Wenbin Wang A pan-genotypic indirect competitive ELISA for serological detection of pigeon circovirus antibodies Frontiers in Microbiology indirect competitive ELISA pigeon circovirus Cap virus-like particles monoclonal antibody |
| title | A pan-genotypic indirect competitive ELISA for serological detection of pigeon circovirus antibodies |
| title_full | A pan-genotypic indirect competitive ELISA for serological detection of pigeon circovirus antibodies |
| title_fullStr | A pan-genotypic indirect competitive ELISA for serological detection of pigeon circovirus antibodies |
| title_full_unstemmed | A pan-genotypic indirect competitive ELISA for serological detection of pigeon circovirus antibodies |
| title_short | A pan-genotypic indirect competitive ELISA for serological detection of pigeon circovirus antibodies |
| title_sort | pan genotypic indirect competitive elisa for serological detection of pigeon circovirus antibodies |
| topic | indirect competitive ELISA pigeon circovirus Cap virus-like particles monoclonal antibody |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1612715/full |
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