Identification and comparative analysis of genes in response to cold stress between two mainly cultivated tetraploid cottons
In order to further explore the genes related to cold tolerance in cotton, we utilized transcriptome sequencing of leaf tissues of TM-1 (Gossypium hirsutum) and Hai7124 (G. barbadense) under cold treatment (4 ℃) and the control treatment (28 ℃) at different stages to compare and analyze their differ...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2022-02-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.04.071 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849419424549830656 |
|---|---|
| author | QI Bowen GUO Menglan LU Hejun MEI Huan ZHAO Ting FANG Lei |
| author_facet | QI Bowen GUO Menglan LU Hejun MEI Huan ZHAO Ting FANG Lei |
| author_sort | QI Bowen |
| collection | DOAJ |
| description | In order to further explore the genes related to cold tolerance in cotton, we utilized transcriptome sequencing of leaf tissues of TM-1 (Gossypium hirsutum) and Hai7124 (G. barbadense) under cold treatment (4 ℃) and the control treatment (28 ℃) at different stages to compare and analyze their differences in cold tolerance related gene’s functions, expression patterns, and metabolic pathways. The results showed that Hai7124 was more sensitive to cold stress than TM-1, and its phenotype changed faster and more obviously under cold stress. Transcriptome analysis showed that TM-1 and Hai7124 contained 8 713 and 12 461 genes in response to cold stress, respectively. According to the orthologs between TM-1 and Hai7124, there were 1 518 and 3 577 specific response genes in TM-1 and Hai7124, respectively. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the specific response genes in TM-1 were mostly involved in hormone signal transduction, circadian rhythm, and other regulation processes, while specific response genes in Hai7124 were mostly involved in amino acid metabolism processes such as lysine, arginine, serine and threonine. Fatty acid metabolism genes related to cold tolerance and genes in key families of fatty acid synthesis pathways, such as acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), stearoyl-ACP desaturase (SAD), fatty acid desaturase (FAD), long chain acyl-CoA synthetase (LACS) from TM-1 and Hai7124 were further analyzed. During the whole cold stress treatment, TM-1 had 11 up-regulated genes, while Hai7124 had 10 down-regulated genes. The real time quantitative reverse polymerase chain reaction (RT-qPCR) was used to verify expression levels of five response genes. It was found that their expression levels were highly correlated with the expression levels of transcriptome. This study is helpful to analyze the molecular mechanism of different cold tolerance of TM-1 and Hai7124 and provide important genetic resources for the improvement of cold tolerance in cotton breeding. |
| format | Article |
| id | doaj-art-2fc6a06ccdd24378843028d262513e2c |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2022-02-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-2fc6a06ccdd24378843028d262513e2c2025-08-20T03:32:05ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552022-02-0148102010.3785/j.issn.1008-9209.2021.04.07110089209Identification and comparative analysis of genes in response to cold stress between two mainly cultivated tetraploid cottonsQI BowenGUO MenglanLU HejunMEI HuanZHAO TingFANG LeiIn order to further explore the genes related to cold tolerance in cotton, we utilized transcriptome sequencing of leaf tissues of TM-1 (Gossypium hirsutum) and Hai7124 (G. barbadense) under cold treatment (4 ℃) and the control treatment (28 ℃) at different stages to compare and analyze their differences in cold tolerance related gene’s functions, expression patterns, and metabolic pathways. The results showed that Hai7124 was more sensitive to cold stress than TM-1, and its phenotype changed faster and more obviously under cold stress. Transcriptome analysis showed that TM-1 and Hai7124 contained 8 713 and 12 461 genes in response to cold stress, respectively. According to the orthologs between TM-1 and Hai7124, there were 1 518 and 3 577 specific response genes in TM-1 and Hai7124, respectively. Gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the specific response genes in TM-1 were mostly involved in hormone signal transduction, circadian rhythm, and other regulation processes, while specific response genes in Hai7124 were mostly involved in amino acid metabolism processes such as lysine, arginine, serine and threonine. Fatty acid metabolism genes related to cold tolerance and genes in key families of fatty acid synthesis pathways, such as acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), stearoyl-ACP desaturase (SAD), fatty acid desaturase (FAD), long chain acyl-CoA synthetase (LACS) from TM-1 and Hai7124 were further analyzed. During the whole cold stress treatment, TM-1 had 11 up-regulated genes, while Hai7124 had 10 down-regulated genes. The real time quantitative reverse polymerase chain reaction (RT-qPCR) was used to verify expression levels of five response genes. It was found that their expression levels were highly correlated with the expression levels of transcriptome. This study is helpful to analyze the molecular mechanism of different cold tolerance of TM-1 and Hai7124 and provide important genetic resources for the improvement of cold tolerance in cotton breeding.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.04.071<italic>Gossypium hirsutum</italic> <italic>Gossypium barbadense</italic> transcriptome sequencingcold stressdifferentially expressed genefatty acid synthesis pathway |
| spellingShingle | QI Bowen GUO Menglan LU Hejun MEI Huan ZHAO Ting FANG Lei Identification and comparative analysis of genes in response to cold stress between two mainly cultivated tetraploid cottons 浙江大学学报. 农业与生命科学版 <italic>Gossypium hirsutum</italic> <italic>Gossypium barbadense</italic> transcriptome sequencing cold stress differentially expressed gene fatty acid synthesis pathway |
| title | Identification and comparative analysis of genes in response to cold stress between two mainly cultivated tetraploid cottons |
| title_full | Identification and comparative analysis of genes in response to cold stress between two mainly cultivated tetraploid cottons |
| title_fullStr | Identification and comparative analysis of genes in response to cold stress between two mainly cultivated tetraploid cottons |
| title_full_unstemmed | Identification and comparative analysis of genes in response to cold stress between two mainly cultivated tetraploid cottons |
| title_short | Identification and comparative analysis of genes in response to cold stress between two mainly cultivated tetraploid cottons |
| title_sort | identification and comparative analysis of genes in response to cold stress between two mainly cultivated tetraploid cottons |
| topic | <italic>Gossypium hirsutum</italic> <italic>Gossypium barbadense</italic> transcriptome sequencing cold stress differentially expressed gene fatty acid synthesis pathway |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.04.071 |
| work_keys_str_mv | AT qibowen identificationandcomparativeanalysisofgenesinresponsetocoldstressbetweentwomainlycultivatedtetraploidcottons AT guomenglan identificationandcomparativeanalysisofgenesinresponsetocoldstressbetweentwomainlycultivatedtetraploidcottons AT luhejun identificationandcomparativeanalysisofgenesinresponsetocoldstressbetweentwomainlycultivatedtetraploidcottons AT meihuan identificationandcomparativeanalysisofgenesinresponsetocoldstressbetweentwomainlycultivatedtetraploidcottons AT zhaoting identificationandcomparativeanalysisofgenesinresponsetocoldstressbetweentwomainlycultivatedtetraploidcottons AT fanglei identificationandcomparativeanalysisofgenesinresponsetocoldstressbetweentwomainlycultivatedtetraploidcottons |