Structure characterization and mechanism of angiotensin I-converting enzyme (ACE) inhibitory peptides modified by plastein reaction derived from tiger nut (Cyperus esculentus)

The development of peptides derived from plants, which have potential anti-angiotensin converting enzyme (ACE) activity and other bioactivities, are of scientific interest. ACE inhibitory peptide (CLPP, the ACE inhibitory rate is 76.52 ± 1.07%) was obtained by hydrolysis of tiger nut (Cyperus escule...

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Main Authors: Junhua Shi, Zhuo Yang, Ming Xu, Guogang Zhao, Yawen Gao, Hongyan Zheng, Ji Feng
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-01-01
Series:Frontiers in Sustainable Food Systems
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Online Access:https://www.frontiersin.org/articles/10.3389/fsufs.2024.1476134/full
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author Junhua Shi
Zhuo Yang
Ming Xu
Guogang Zhao
Yawen Gao
Hongyan Zheng
Ji Feng
author_facet Junhua Shi
Zhuo Yang
Ming Xu
Guogang Zhao
Yawen Gao
Hongyan Zheng
Ji Feng
author_sort Junhua Shi
collection DOAJ
description The development of peptides derived from plants, which have potential anti-angiotensin converting enzyme (ACE) activity and other bioactivities, are of scientific interest. ACE inhibitory peptide (CLPP, the ACE inhibitory rate is 76.52 ± 1.07%) was obtained by hydrolysis of tiger nut (Cyperus esculentus) protein with alkaline protease. The plastein product (PCLPP, the ACE inhibitory rate is 85 ± 2.33%) was prepared by modifying CLPP with a plastein reaction. The results of SEM, IR, and XRD show that the structure of PCLPP is different from that of CLPP. The results of differential scanning calorimetry, fluorescence, and free amino analysis showed that the reaction was carried out by aggregation and condensation. These results suggest that the plastein reaction may be an effective method to increase the variety of bioactive peptides.
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institution DOAJ
issn 2571-581X
language English
publishDate 2025-01-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Sustainable Food Systems
spelling doaj-art-2fc4ee1a4889440882b6afb827a219a22025-08-20T03:01:35ZengFrontiers Media S.A.Frontiers in Sustainable Food Systems2571-581X2025-01-01810.3389/fsufs.2024.14761341476134Structure characterization and mechanism of angiotensin I-converting enzyme (ACE) inhibitory peptides modified by plastein reaction derived from tiger nut (Cyperus esculentus)Junhua Shi0Zhuo Yang1Ming Xu2Guogang Zhao3Yawen Gao4Hongyan Zheng5Ji Feng6College of Food Science and Engineering, Jilin Agricultural University, Changchun, ChinaCollege of Food Science and Engineering, Jilin Agricultural University, Changchun, ChinaCollege of Food Science and Engineering, Jilin Agricultural University, Changchun, ChinaCybersecurity Academy, Changchun University, Changchun, ChinaCollege of Food Science and Engineering, Jilin Agricultural University, Changchun, ChinaCollege of Food Science and Engineering, Jilin Agricultural University, Changchun, ChinaJilin Province Product Quality Supervision and Inspection Institute, Changchun, ChinaThe development of peptides derived from plants, which have potential anti-angiotensin converting enzyme (ACE) activity and other bioactivities, are of scientific interest. ACE inhibitory peptide (CLPP, the ACE inhibitory rate is 76.52 ± 1.07%) was obtained by hydrolysis of tiger nut (Cyperus esculentus) protein with alkaline protease. The plastein product (PCLPP, the ACE inhibitory rate is 85 ± 2.33%) was prepared by modifying CLPP with a plastein reaction. The results of SEM, IR, and XRD show that the structure of PCLPP is different from that of CLPP. The results of differential scanning calorimetry, fluorescence, and free amino analysis showed that the reaction was carried out by aggregation and condensation. These results suggest that the plastein reaction may be an effective method to increase the variety of bioactive peptides.https://www.frontiersin.org/articles/10.3389/fsufs.2024.1476134/fullplastein reactiontrypsinpeptidesangiotensin converting enzymetiger nut
spellingShingle Junhua Shi
Zhuo Yang
Ming Xu
Guogang Zhao
Yawen Gao
Hongyan Zheng
Ji Feng
Structure characterization and mechanism of angiotensin I-converting enzyme (ACE) inhibitory peptides modified by plastein reaction derived from tiger nut (Cyperus esculentus)
Frontiers in Sustainable Food Systems
plastein reaction
trypsin
peptides
angiotensin converting enzyme
tiger nut
title Structure characterization and mechanism of angiotensin I-converting enzyme (ACE) inhibitory peptides modified by plastein reaction derived from tiger nut (Cyperus esculentus)
title_full Structure characterization and mechanism of angiotensin I-converting enzyme (ACE) inhibitory peptides modified by plastein reaction derived from tiger nut (Cyperus esculentus)
title_fullStr Structure characterization and mechanism of angiotensin I-converting enzyme (ACE) inhibitory peptides modified by plastein reaction derived from tiger nut (Cyperus esculentus)
title_full_unstemmed Structure characterization and mechanism of angiotensin I-converting enzyme (ACE) inhibitory peptides modified by plastein reaction derived from tiger nut (Cyperus esculentus)
title_short Structure characterization and mechanism of angiotensin I-converting enzyme (ACE) inhibitory peptides modified by plastein reaction derived from tiger nut (Cyperus esculentus)
title_sort structure characterization and mechanism of angiotensin i converting enzyme ace inhibitory peptides modified by plastein reaction derived from tiger nut cyperus esculentus
topic plastein reaction
trypsin
peptides
angiotensin converting enzyme
tiger nut
url https://www.frontiersin.org/articles/10.3389/fsufs.2024.1476134/full
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