A tailored lectin microarray for rapid glycan profiling of therapeutic monoclonal antibodies
Glycosylation plays a crucial role in determining the quality and efficacy of therapeutic antibodies. This necessitates a thorough analysis and monitoring process to ensure consistent product quality during manufacturing. In this study, we introduce a custom-designed lectin microarray featuring nine...
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| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | mAbs |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19420862.2024.2304268 |
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| author | Shen Luo Baolin Zhang |
| author_facet | Shen Luo Baolin Zhang |
| author_sort | Shen Luo |
| collection | DOAJ |
| description | Glycosylation plays a crucial role in determining the quality and efficacy of therapeutic antibodies. This necessitates a thorough analysis and monitoring process to ensure consistent product quality during manufacturing. In this study, we introduce a custom-designed lectin microarray featuring nine distinct lectins: rPhoSL, rOTH3, RCA120, rMan2, MAL_I, rPSL1a, PHAE, rMOA, and PHAL. These lectins have been specifically tailored to selectively bind to common N-glycan epitopes found in therapeutic IgG antibodies. By utilizing intact glycoprotein samples, our nine-lectin microarray provides a high-throughput platform for rapid glycan profiling, enabling comparative analysis of glycosylation patterns. Our results demonstrate the practical utility of this microarray in assessing glycosylation across various manufacturing batches or between biosimilar and innovator products. This capacity empowers informed decision-making in the development and production of therapeutic antibodies. |
| format | Article |
| id | doaj-art-2fc45c33d15146f88c04bfa0f7ddf063 |
| institution | Kabale University |
| issn | 1942-0862 1942-0870 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | mAbs |
| spelling | doaj-art-2fc45c33d15146f88c04bfa0f7ddf0632025-01-31T04:19:38ZengTaylor & Francis GroupmAbs1942-08621942-08702024-12-0116110.1080/19420862.2024.2304268A tailored lectin microarray for rapid glycan profiling of therapeutic monoclonal antibodiesShen Luo0Baolin Zhang1Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USAOffice of Pharmaceutical Quality, Center for Drug Evaluation and Research, Food and Drug Administration, Silver Spring, MD, USAGlycosylation plays a crucial role in determining the quality and efficacy of therapeutic antibodies. This necessitates a thorough analysis and monitoring process to ensure consistent product quality during manufacturing. In this study, we introduce a custom-designed lectin microarray featuring nine distinct lectins: rPhoSL, rOTH3, RCA120, rMan2, MAL_I, rPSL1a, PHAE, rMOA, and PHAL. These lectins have been specifically tailored to selectively bind to common N-glycan epitopes found in therapeutic IgG antibodies. By utilizing intact glycoprotein samples, our nine-lectin microarray provides a high-throughput platform for rapid glycan profiling, enabling comparative analysis of glycosylation patterns. Our results demonstrate the practical utility of this microarray in assessing glycosylation across various manufacturing batches or between biosimilar and innovator products. This capacity empowers informed decision-making in the development and production of therapeutic antibodies.https://www.tandfonline.com/doi/10.1080/19420862.2024.2304268Bispecific antibodyglycan profileglycosylationlectin microarraymonoclonal antibodyproduct quality |
| spellingShingle | Shen Luo Baolin Zhang A tailored lectin microarray for rapid glycan profiling of therapeutic monoclonal antibodies mAbs Bispecific antibody glycan profile glycosylation lectin microarray monoclonal antibody product quality |
| title | A tailored lectin microarray for rapid glycan profiling of therapeutic monoclonal antibodies |
| title_full | A tailored lectin microarray for rapid glycan profiling of therapeutic monoclonal antibodies |
| title_fullStr | A tailored lectin microarray for rapid glycan profiling of therapeutic monoclonal antibodies |
| title_full_unstemmed | A tailored lectin microarray for rapid glycan profiling of therapeutic monoclonal antibodies |
| title_short | A tailored lectin microarray for rapid glycan profiling of therapeutic monoclonal antibodies |
| title_sort | tailored lectin microarray for rapid glycan profiling of therapeutic monoclonal antibodies |
| topic | Bispecific antibody glycan profile glycosylation lectin microarray monoclonal antibody product quality |
| url | https://www.tandfonline.com/doi/10.1080/19420862.2024.2304268 |
| work_keys_str_mv | AT shenluo atailoredlectinmicroarrayforrapidglycanprofilingoftherapeuticmonoclonalantibodies AT baolinzhang atailoredlectinmicroarrayforrapidglycanprofilingoftherapeuticmonoclonalantibodies AT shenluo tailoredlectinmicroarrayforrapidglycanprofilingoftherapeuticmonoclonalantibodies AT baolinzhang tailoredlectinmicroarrayforrapidglycanprofilingoftherapeuticmonoclonalantibodies |