Reduction of IL-17A Might Suppress the Th1 Response and Promote the Th2 Response by Boosting the Function of Treg Cells during Silica-Induced Inflammatory Response In Vitro
Silica inhalation can induce chronic lung inflammation and fibrosis. Upon silica stimulation, activated macrophages trigger the T-lymphocyte which can differentiate into many different types of Th cells, including the recently discovered Th17 cells. IL-17A, the typical Th17 cytokine, is reported in...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/570894 |
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| author | Wen Tang Fangwei Liu Ying Chen Laiyu Song Wujing Dai Chao Li Dong Weng Jie Chen |
| author_facet | Wen Tang Fangwei Liu Ying Chen Laiyu Song Wujing Dai Chao Li Dong Weng Jie Chen |
| author_sort | Wen Tang |
| collection | DOAJ |
| description | Silica inhalation can induce chronic lung inflammation and fibrosis. Upon silica stimulation, activated macrophages trigger the T-lymphocyte which can differentiate into many different types of Th cells, including the recently discovered Th17 cells. IL-17A, the typical Th17 cytokine, is reported in some inflammatory diseases. However, the role of IL-17A in silica-induced inflammatory response is still not clear. The regulatory mechanism of silica-induced Th17 response also needs to be investigated. So we established a mice primary cell coculture system (macrophage and lymphocyte) to investigate the role of IL-17A in silica-induced inflammatory response in vitro, by using anti-IL-17A mAb and IL-1Ra. Both anti-IL-17A mAb and IL-1Ra decreased the level of IL-17A and increased the function of Treg cells. The Th1 response was suppressed and the Th2 response was promoted by the addition of anti-IL-17A mAb or IL-1Ra. IL-1Ra treatment decreased the level of IL-6, whereas the levels of IL-23 and ROR-γt were increased. Our study demonstrated that IL-17A reduction altered the pattern of silica-induced Th responses by boosting the function of Treg cells in vitro. Blocking the function of IL-1 signal pathway could suppress the level of IL-17A, which played the major role in modulating silica-induced Th responses in vitro. |
| format | Article |
| id | doaj-art-2fc1eed0145b46b7843f07d43b2f1ff8 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-2fc1eed0145b46b7843f07d43b2f1ff82025-02-03T05:44:49ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/570894570894Reduction of IL-17A Might Suppress the Th1 Response and Promote the Th2 Response by Boosting the Function of Treg Cells during Silica-Induced Inflammatory Response In VitroWen Tang0Fangwei Liu1Ying Chen2Laiyu Song3Wujing Dai4Chao Li5Dong Weng6Jie Chen7Division of Pneumoconiosis, School of Public Health, China Medical University, No. 92 North 2nd Road, Heping District, Shenyang, Liaoning 110001, ChinaDivision of Pneumoconiosis, School of Public Health, China Medical University, No. 92 North 2nd Road, Heping District, Shenyang, Liaoning 110001, ChinaDivision of Pneumoconiosis, School of Public Health, China Medical University, No. 92 North 2nd Road, Heping District, Shenyang, Liaoning 110001, ChinaDivision of Pneumoconiosis, School of Public Health, China Medical University, No. 92 North 2nd Road, Heping District, Shenyang, Liaoning 110001, ChinaDivision of Pneumoconiosis, School of Public Health, China Medical University, No. 92 North 2nd Road, Heping District, Shenyang, Liaoning 110001, ChinaDivision of Pneumoconiosis, School of Public Health, China Medical University, No. 92 North 2nd Road, Heping District, Shenyang, Liaoning 110001, ChinaDivision of Pneumoconiosis, School of Public Health, China Medical University, No. 92 North 2nd Road, Heping District, Shenyang, Liaoning 110001, ChinaDivision of Pneumoconiosis, School of Public Health, China Medical University, No. 92 North 2nd Road, Heping District, Shenyang, Liaoning 110001, ChinaSilica inhalation can induce chronic lung inflammation and fibrosis. Upon silica stimulation, activated macrophages trigger the T-lymphocyte which can differentiate into many different types of Th cells, including the recently discovered Th17 cells. IL-17A, the typical Th17 cytokine, is reported in some inflammatory diseases. However, the role of IL-17A in silica-induced inflammatory response is still not clear. The regulatory mechanism of silica-induced Th17 response also needs to be investigated. So we established a mice primary cell coculture system (macrophage and lymphocyte) to investigate the role of IL-17A in silica-induced inflammatory response in vitro, by using anti-IL-17A mAb and IL-1Ra. Both anti-IL-17A mAb and IL-1Ra decreased the level of IL-17A and increased the function of Treg cells. The Th1 response was suppressed and the Th2 response was promoted by the addition of anti-IL-17A mAb or IL-1Ra. IL-1Ra treatment decreased the level of IL-6, whereas the levels of IL-23 and ROR-γt were increased. Our study demonstrated that IL-17A reduction altered the pattern of silica-induced Th responses by boosting the function of Treg cells in vitro. Blocking the function of IL-1 signal pathway could suppress the level of IL-17A, which played the major role in modulating silica-induced Th responses in vitro.http://dx.doi.org/10.1155/2014/570894 |
| spellingShingle | Wen Tang Fangwei Liu Ying Chen Laiyu Song Wujing Dai Chao Li Dong Weng Jie Chen Reduction of IL-17A Might Suppress the Th1 Response and Promote the Th2 Response by Boosting the Function of Treg Cells during Silica-Induced Inflammatory Response In Vitro Mediators of Inflammation |
| title | Reduction of IL-17A Might Suppress the Th1 Response and Promote the Th2 Response by Boosting the Function of Treg Cells during Silica-Induced Inflammatory Response In Vitro |
| title_full | Reduction of IL-17A Might Suppress the Th1 Response and Promote the Th2 Response by Boosting the Function of Treg Cells during Silica-Induced Inflammatory Response In Vitro |
| title_fullStr | Reduction of IL-17A Might Suppress the Th1 Response and Promote the Th2 Response by Boosting the Function of Treg Cells during Silica-Induced Inflammatory Response In Vitro |
| title_full_unstemmed | Reduction of IL-17A Might Suppress the Th1 Response and Promote the Th2 Response by Boosting the Function of Treg Cells during Silica-Induced Inflammatory Response In Vitro |
| title_short | Reduction of IL-17A Might Suppress the Th1 Response and Promote the Th2 Response by Boosting the Function of Treg Cells during Silica-Induced Inflammatory Response In Vitro |
| title_sort | reduction of il 17a might suppress the th1 response and promote the th2 response by boosting the function of treg cells during silica induced inflammatory response in vitro |
| url | http://dx.doi.org/10.1155/2014/570894 |
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