BLM Deficiency Is Not Associated with Sensitivity to Hydroxyurea-Induced Replication Stress
Bloom's syndrome (BS) displays one of the strongest known correlations between chromosomal instability and a high risk of cancer at an early age. BS cells combine a reduced average fork velocity with constitutive endogenous replication stress. However, the response of BS cells to replication st...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.4061/2010/319754 |
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| _version_ | 1850216970476060672 |
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| author | Kenza Lahkim Bennani-Belhaj Géraldine Buhagiar-Labarchède Nada Jmari Rosine Onclercq-Delic Mounira Amor-Guéret |
| author_facet | Kenza Lahkim Bennani-Belhaj Géraldine Buhagiar-Labarchède Nada Jmari Rosine Onclercq-Delic Mounira Amor-Guéret |
| author_sort | Kenza Lahkim Bennani-Belhaj |
| collection | DOAJ |
| description | Bloom's syndrome (BS) displays one of the strongest known correlations between chromosomal instability and a high risk of cancer at an early age. BS cells combine a reduced average fork velocity with constitutive endogenous replication stress. However, the response of BS cells to replication stress induced by hydroxyurea (HU), which strongly slows the progression of replication forks, remains unclear due to publication of conflicting results. Using two different cellular models of BS, we showed that BLM deficiency is not associated with sensitivity to HU, in terms of clonogenic survival, DSB generation, and SCE induction. We suggest that surviving BLM-deficient cells are selected on the basis of their ability to deal with an endogenous replication stress induced by replication fork slowing, resulting in insensitivity to HU-induced replication stress. |
| format | Article |
| id | doaj-art-2fbeec5a43e34e93a550d1e7b8eb2b7d |
| institution | OA Journals |
| issn | 2090-021X |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-2fbeec5a43e34e93a550d1e7b8eb2b7d2025-08-20T02:08:11ZengWileyJournal of Nucleic Acids2090-021X2010-01-01201010.4061/2010/319754319754BLM Deficiency Is Not Associated with Sensitivity to Hydroxyurea-Induced Replication StressKenza Lahkim Bennani-Belhaj0Géraldine Buhagiar-Labarchède1Nada Jmari2Rosine Onclercq-Delic3Mounira Amor-Guéret4Institut Curie, Centre de Recherche, Centre Universitaire, Bât. 110, 91405 Orsay, FranceInstitut Curie, Centre de Recherche, Centre Universitaire, Bât. 110, 91405 Orsay, FranceInstitut Curie, Centre de Recherche, Centre Universitaire, Bât. 110, 91405 Orsay, FranceInstitut Curie, Centre de Recherche, Centre Universitaire, Bât. 110, 91405 Orsay, FranceInstitut Curie, Centre de Recherche, Centre Universitaire, Bât. 110, 91405 Orsay, FranceBloom's syndrome (BS) displays one of the strongest known correlations between chromosomal instability and a high risk of cancer at an early age. BS cells combine a reduced average fork velocity with constitutive endogenous replication stress. However, the response of BS cells to replication stress induced by hydroxyurea (HU), which strongly slows the progression of replication forks, remains unclear due to publication of conflicting results. Using two different cellular models of BS, we showed that BLM deficiency is not associated with sensitivity to HU, in terms of clonogenic survival, DSB generation, and SCE induction. We suggest that surviving BLM-deficient cells are selected on the basis of their ability to deal with an endogenous replication stress induced by replication fork slowing, resulting in insensitivity to HU-induced replication stress.http://dx.doi.org/10.4061/2010/319754 |
| spellingShingle | Kenza Lahkim Bennani-Belhaj Géraldine Buhagiar-Labarchède Nada Jmari Rosine Onclercq-Delic Mounira Amor-Guéret BLM Deficiency Is Not Associated with Sensitivity to Hydroxyurea-Induced Replication Stress Journal of Nucleic Acids |
| title | BLM Deficiency Is Not Associated with Sensitivity to
Hydroxyurea-Induced Replication Stress |
| title_full | BLM Deficiency Is Not Associated with Sensitivity to
Hydroxyurea-Induced Replication Stress |
| title_fullStr | BLM Deficiency Is Not Associated with Sensitivity to
Hydroxyurea-Induced Replication Stress |
| title_full_unstemmed | BLM Deficiency Is Not Associated with Sensitivity to
Hydroxyurea-Induced Replication Stress |
| title_short | BLM Deficiency Is Not Associated with Sensitivity to
Hydroxyurea-Induced Replication Stress |
| title_sort | blm deficiency is not associated with sensitivity to hydroxyurea induced replication stress |
| url | http://dx.doi.org/10.4061/2010/319754 |
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