Evolution of the umbilical cord blood proteome across gestational development
Abstract Neonatal health is dependent on early risk stratification, diagnosis, and timely management of potentially devastating conditions, particularly in the setting of prematurity. Many of these conditions are poorly predicted in real-time by clinical data and current diagnostics. Umbilical cord...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-84446-5 |
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| author | Leena B. Mithal Nicola Lancki Ted Ling-Hu Young Ah Goo Sebastian Otero Nathaniel J. Rhodes Byoung-Kyu Cho William A. Grobman Judd F. Hultquist Denise Scholtens Karen K. Mestan Patrick C. Seed |
| author_facet | Leena B. Mithal Nicola Lancki Ted Ling-Hu Young Ah Goo Sebastian Otero Nathaniel J. Rhodes Byoung-Kyu Cho William A. Grobman Judd F. Hultquist Denise Scholtens Karen K. Mestan Patrick C. Seed |
| author_sort | Leena B. Mithal |
| collection | DOAJ |
| description | Abstract Neonatal health is dependent on early risk stratification, diagnosis, and timely management of potentially devastating conditions, particularly in the setting of prematurity. Many of these conditions are poorly predicted in real-time by clinical data and current diagnostics. Umbilical cord blood may represent a novel source of molecular signatures that provides a window into the state of the fetus at birth. In this study, we comprehensively characterized the cord blood proteome of infants born between 25 to 42 weeks using untargeted mass spectrometry and functional enrichment analysis. We determined that the cord blood proteome at birth varies significantly across gestational development. Proteins that function in structural development and growth (e.g., extracellular matrix organization, lipid particle remodeling, and blood vessel development) are more abundant earlier in gestation. In later gestations, proteins with increased abundance are in immune response and inflammatory pathways, including complements and calcium-binding proteins. These data contribute to the knowledge of the physiologic state of neonates across gestational age, which is crucial to understand as we strive to best support postnatal development in preterm infants, determine mechanisms of pathology causing adverse health outcomes, and develop cord blood biomarkers to help tailor our diagnosis and therapeutics for critical neonatal conditions. |
| format | Article |
| id | doaj-art-2fbb8436fd6e4e8c9ab123c768653b46 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-2fbb8436fd6e4e8c9ab123c768653b462025-01-19T12:18:51ZengNature PortfolioScientific Reports2045-23222025-01-0115111010.1038/s41598-024-84446-5Evolution of the umbilical cord blood proteome across gestational developmentLeena B. Mithal0Nicola Lancki1Ted Ling-Hu2Young Ah Goo3Sebastian Otero4Nathaniel J. Rhodes5Byoung-Kyu Cho6William A. Grobman7Judd F. Hultquist8Denise Scholtens9Karen K. Mestan10Patrick C. Seed11Department of Pediatrics, Division of Infectious Diseases, Ann & Robert H. Lurie Children’s Hospital of Chicago, Northwestern University Feinberg School of MedicineDepartment of Preventive Medicine, Division of Biostatistics, Northwestern University Feinberg School of MedicineDepartment of Medicine, Division of Infectious Diseases, Northwestern University Feinberg School of MedicineMass Spectrometry Technology Access Center at McDonnell Genome Institute (MTAC@MGI), Washington University in Saint Louis School of MedicineDepartment of Pediatrics, Division of Infectious Diseases, Ann & Robert H. Lurie Children’s Hospital of Chicago, Northwestern University Feinberg School of MedicineDepartment of Pharmacy Practice, College of Pharmacy, Midwestern UniversityMass Spectrometry Technology Access Center at McDonnell Genome Institute (MTAC@MGI), Washington University in Saint Louis School of MedicineDepartment of Obstetrics and Gynecology, Ohio State UniversityDepartment of Medicine, Division of Infectious Diseases, Northwestern University Feinberg School of MedicineDepartment of Preventive Medicine, Division of Biostatistics, Northwestern University Feinberg School of MedicineDepartment of Pediatrics, Division of Neonatology, University of California San DiegoDepartment of Pediatrics, Division of Infectious Diseases, Ann & Robert H. Lurie Children’s Hospital of Chicago, Northwestern University Feinberg School of MedicineAbstract Neonatal health is dependent on early risk stratification, diagnosis, and timely management of potentially devastating conditions, particularly in the setting of prematurity. Many of these conditions are poorly predicted in real-time by clinical data and current diagnostics. Umbilical cord blood may represent a novel source of molecular signatures that provides a window into the state of the fetus at birth. In this study, we comprehensively characterized the cord blood proteome of infants born between 25 to 42 weeks using untargeted mass spectrometry and functional enrichment analysis. We determined that the cord blood proteome at birth varies significantly across gestational development. Proteins that function in structural development and growth (e.g., extracellular matrix organization, lipid particle remodeling, and blood vessel development) are more abundant earlier in gestation. In later gestations, proteins with increased abundance are in immune response and inflammatory pathways, including complements and calcium-binding proteins. These data contribute to the knowledge of the physiologic state of neonates across gestational age, which is crucial to understand as we strive to best support postnatal development in preterm infants, determine mechanisms of pathology causing adverse health outcomes, and develop cord blood biomarkers to help tailor our diagnosis and therapeutics for critical neonatal conditions.https://doi.org/10.1038/s41598-024-84446-5Cord bloodProteomicsNeonatal immunologyPrematurityImmune developmentBiomarker development |
| spellingShingle | Leena B. Mithal Nicola Lancki Ted Ling-Hu Young Ah Goo Sebastian Otero Nathaniel J. Rhodes Byoung-Kyu Cho William A. Grobman Judd F. Hultquist Denise Scholtens Karen K. Mestan Patrick C. Seed Evolution of the umbilical cord blood proteome across gestational development Scientific Reports Cord blood Proteomics Neonatal immunology Prematurity Immune development Biomarker development |
| title | Evolution of the umbilical cord blood proteome across gestational development |
| title_full | Evolution of the umbilical cord blood proteome across gestational development |
| title_fullStr | Evolution of the umbilical cord blood proteome across gestational development |
| title_full_unstemmed | Evolution of the umbilical cord blood proteome across gestational development |
| title_short | Evolution of the umbilical cord blood proteome across gestational development |
| title_sort | evolution of the umbilical cord blood proteome across gestational development |
| topic | Cord blood Proteomics Neonatal immunology Prematurity Immune development Biomarker development |
| url | https://doi.org/10.1038/s41598-024-84446-5 |
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