Effect of gum Arabic as natural prebiotic on intestinal ecosystem of post-hatched broiler chicks

The purpose of the current study was to investigate the effects of gum Arabic supplementation on short-chain fatty acids, cecal microbiota, immune-related gene expression, and small intestinal morphology in post-hatched broiler chicks. On the day of hatching, four hundred thirty-two commercial male...

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Main Authors: Hani Hasan Al-Baadani, Rashed Abdullah Alhotan, Mahmoud Mustafa Azzam, Ibrahim Abdullah Alhidary, Abdulrahman Salem Alharthi, Abdulaziz Abdullah Al-Abdullatif
Format: Article
Language:English
Published: Korean Society of Animal Sciences and Technology 2024-11-01
Series:Journal of Animal Science and Technology
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Online Access:http://www.ejast.org/archive/view_article?doi=10.5187/jast.2023.e57
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author Hani Hasan Al-Baadani
Rashed Abdullah Alhotan
Mahmoud Mustafa Azzam
Ibrahim Abdullah Alhidary
Abdulrahman Salem Alharthi
Abdulaziz Abdullah Al-Abdullatif
author_facet Hani Hasan Al-Baadani
Rashed Abdullah Alhotan
Mahmoud Mustafa Azzam
Ibrahim Abdullah Alhidary
Abdulrahman Salem Alharthi
Abdulaziz Abdullah Al-Abdullatif
author_sort Hani Hasan Al-Baadani
collection DOAJ
description The purpose of the current study was to investigate the effects of gum Arabic supplementation on short-chain fatty acids, cecal microbiota, immune-related gene expression, and small intestinal morphology in post-hatched broiler chicks. On the day of hatching, four hundred thirty-two commercial male broiler chicks were randomly allocated into six treatments with twelve cages as replicates of six chicks each for 24 days. Dietary treatments (T1 to T6) were supplemented with 0.0, 0.12, 0.25, 0.50, 0.75, and 1.0% gum Arabic to the basal diet, respectively. Performance parameters, short-chain fatty acid concentration, quantification of microbiota and immune response gene expression (pre-inflammatory cytokines, mucin-2, and secretory immunoglobulin A), and histomorphometry of the small intestine were measured. According to our results, daily weight gains in T2 and the production efficiency index increased in T2 to T4, whereas daily feed intake decreased in T2, T3, T5, and T6, but feed conversion ratio improved. Concentration of lactate, acetate, butyrate, and total short-chain fatty acid increased in T2, T3, T5, and T6. Propionate ‎in T2 T3, T4, and T6 and format in T2, T5, and T6 also increased. Lactobacillus spp. quantitatively increased from T3 to T6, whereas Bacteroides spp. decreased in T3 and T5. Other microbiota quantitatively showed no effect of dietary supplements. IL-1β, TNF-α, and MUC-2 decreased in T2 to T6 and IL-12 in T3, whereas INF-Y increased in T4 to T6 and SIgA in T4. All histometeric parameters of the duodenum, jejunum, and ileum improved with dietary supplementation. We conclude that the administration of gum Arabic resulted in an improvement in overall performance, fermentation metabolites, and modification of microbiota and immune response with improved histomorphometry in the intestines of young chicks.
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publishDate 2024-11-01
publisher Korean Society of Animal Sciences and Technology
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spelling doaj-art-2fa92899ae834902a4426e80d3f823c92025-08-20T02:50:23ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology2672-01912055-03912024-11-016661203122010.5187/jast.2023.e57Effect of gum Arabic as natural prebiotic on intestinal ecosystem of post-hatched broiler chicksHani Hasan Al-Baadani0Rashed Abdullah Alhotan1Mahmoud Mustafa Azzam2Ibrahim Abdullah Alhidary3Abdulrahman Salem Alharthi4Abdulaziz Abdullah Al-Abdullatif5Department of Animal Production, College of Food and Agriculture Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Animal Production, College of Food and Agriculture Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Animal Production, College of Food and Agriculture Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Animal Production, College of Food and Agriculture Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Animal Production, College of Food and Agriculture Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Animal Production, College of Food and Agriculture Science, King Saud University, Riyadh 11451, Saudi ArabiaThe purpose of the current study was to investigate the effects of gum Arabic supplementation on short-chain fatty acids, cecal microbiota, immune-related gene expression, and small intestinal morphology in post-hatched broiler chicks. On the day of hatching, four hundred thirty-two commercial male broiler chicks were randomly allocated into six treatments with twelve cages as replicates of six chicks each for 24 days. Dietary treatments (T1 to T6) were supplemented with 0.0, 0.12, 0.25, 0.50, 0.75, and 1.0% gum Arabic to the basal diet, respectively. Performance parameters, short-chain fatty acid concentration, quantification of microbiota and immune response gene expression (pre-inflammatory cytokines, mucin-2, and secretory immunoglobulin A), and histomorphometry of the small intestine were measured. According to our results, daily weight gains in T2 and the production efficiency index increased in T2 to T4, whereas daily feed intake decreased in T2, T3, T5, and T6, but feed conversion ratio improved. Concentration of lactate, acetate, butyrate, and total short-chain fatty acid increased in T2, T3, T5, and T6. Propionate ‎in T2 T3, T4, and T6 and format in T2, T5, and T6 also increased. Lactobacillus spp. quantitatively increased from T3 to T6, whereas Bacteroides spp. decreased in T3 and T5. Other microbiota quantitatively showed no effect of dietary supplements. IL-1β, TNF-α, and MUC-2 decreased in T2 to T6 and IL-12 in T3, whereas INF-Y increased in T4 to T6 and SIgA in T4. All histometeric parameters of the duodenum, jejunum, and ileum improved with dietary supplementation. We conclude that the administration of gum Arabic resulted in an improvement in overall performance, fermentation metabolites, and modification of microbiota and immune response with improved histomorphometry in the intestines of young chicks. http://www.ejast.org/archive/view_article?doi=10.5187/jast.2023.e57Gallus domesticusPerformanceShort-chain fatty acids (SCFAs)MicrobiotaImmune responseMorphology
spellingShingle Hani Hasan Al-Baadani
Rashed Abdullah Alhotan
Mahmoud Mustafa Azzam
Ibrahim Abdullah Alhidary
Abdulrahman Salem Alharthi
Abdulaziz Abdullah Al-Abdullatif
Effect of gum Arabic as natural prebiotic on intestinal ecosystem of post-hatched broiler chicks
Journal of Animal Science and Technology
Gallus domesticus
Performance
Short-chain fatty acids (SCFAs)
Microbiota
Immune response
Morphology
title Effect of gum Arabic as natural prebiotic on intestinal ecosystem of post-hatched broiler chicks
title_full Effect of gum Arabic as natural prebiotic on intestinal ecosystem of post-hatched broiler chicks
title_fullStr Effect of gum Arabic as natural prebiotic on intestinal ecosystem of post-hatched broiler chicks
title_full_unstemmed Effect of gum Arabic as natural prebiotic on intestinal ecosystem of post-hatched broiler chicks
title_short Effect of gum Arabic as natural prebiotic on intestinal ecosystem of post-hatched broiler chicks
title_sort effect of gum arabic as natural prebiotic on intestinal ecosystem of post hatched broiler chicks
topic Gallus domesticus
Performance
Short-chain fatty acids (SCFAs)
Microbiota
Immune response
Morphology
url http://www.ejast.org/archive/view_article?doi=10.5187/jast.2023.e57
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