Selection of Infective Arbuscular Mycorrhizal Fungal Isolates for Field Inoculation
Arbuscular mycorrhizal (AM) fungi play a key role in host plant growth and health, nutrient and water uptake, plant community diversity and dynamics. AM fungi differ in their symbiotic performance, which is the result of the interaction of two fungal characters, infectivity and efficiency. Infectivi...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2010-09-01
|
| Series: | Italian Journal of Agronomy |
| Online Access: | https://www.agronomy.it/index.php/agro/article/view/7 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850265190729252864 |
|---|---|
| author | Elisa Pellegrino Chandra Kamatchi Ramasamy Cristiana Sbrana Paolo Barberi Manuela Giovannetti |
| author_facet | Elisa Pellegrino Chandra Kamatchi Ramasamy Cristiana Sbrana Paolo Barberi Manuela Giovannetti |
| author_sort | Elisa Pellegrino |
| collection | DOAJ |
| description | Arbuscular mycorrhizal (AM) fungi play a key role in host plant growth and health, nutrient and water uptake, plant community diversity and dynamics. AM fungi differ in their symbiotic performance, which is the result of the interaction of two fungal characters, infectivity and efficiency. Infectivity is the ability of a fungal isolate to establish rapidly an extensive mycorrhizal symbiosis and is correlated with pre-symbiotic steps of fungal life cycle, such as spore germination and hyphal growth. Here, different AM fungal isolates were tested, with the aim of selecting infective endophytes for field inoculation. Greenhouse and microcosm experiments were performed in order to assess the ability of 12 AM fungal isolates to produce spores, colonize host roots and to perform initial steps of symbiosis establishment, such as spore germination and hyphal growth. AM fungal spore production and root colonization were significantly different among AM fungal isolates. Spore and sporocarp densities ranged from 0.8 to 7.4 and from 0.6 to 2.0 per gram of soil, respectively, whereas root colonization ranged from 2.9 to 72.2%. Percentage of spore or sporocarp germination ranged from 5.8 to 53.3% and hyphal length from 4.7 to 79.8 mm. The ordination analysis (Redundancy Analysis, RDA) showed that environmental factors explained about 60% of the whole variance and their effect on fungal infectivity variables was significant (P = 0.002). The biplot clearly showed that variables which might be used to detect infective AM fungal isolates were hyphal length and root colonization. Such analysis may allow the detection of the best parameters to select efficient AM fungal isolates to be used in agriculture. |
| format | Article |
| id | doaj-art-2fa53fecb196498597ff9613013e8772 |
| institution | OA Journals |
| issn | 1125-4718 2039-6805 |
| language | English |
| publishDate | 2010-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Italian Journal of Agronomy |
| spelling | doaj-art-2fa53fecb196498597ff9613013e87722025-08-20T01:54:30ZengElsevierItalian Journal of Agronomy1125-47182039-68052010-09-015310.4081/ija.2010.2251Selection of Infective Arbuscular Mycorrhizal Fungal Isolates for Field InoculationElisa PellegrinoChandra Kamatchi RamasamyCristiana SbranaPaolo BarberiManuela GiovannettiArbuscular mycorrhizal (AM) fungi play a key role in host plant growth and health, nutrient and water uptake, plant community diversity and dynamics. AM fungi differ in their symbiotic performance, which is the result of the interaction of two fungal characters, infectivity and efficiency. Infectivity is the ability of a fungal isolate to establish rapidly an extensive mycorrhizal symbiosis and is correlated with pre-symbiotic steps of fungal life cycle, such as spore germination and hyphal growth. Here, different AM fungal isolates were tested, with the aim of selecting infective endophytes for field inoculation. Greenhouse and microcosm experiments were performed in order to assess the ability of 12 AM fungal isolates to produce spores, colonize host roots and to perform initial steps of symbiosis establishment, such as spore germination and hyphal growth. AM fungal spore production and root colonization were significantly different among AM fungal isolates. Spore and sporocarp densities ranged from 0.8 to 7.4 and from 0.6 to 2.0 per gram of soil, respectively, whereas root colonization ranged from 2.9 to 72.2%. Percentage of spore or sporocarp germination ranged from 5.8 to 53.3% and hyphal length from 4.7 to 79.8 mm. The ordination analysis (Redundancy Analysis, RDA) showed that environmental factors explained about 60% of the whole variance and their effect on fungal infectivity variables was significant (P = 0.002). The biplot clearly showed that variables which might be used to detect infective AM fungal isolates were hyphal length and root colonization. Such analysis may allow the detection of the best parameters to select efficient AM fungal isolates to be used in agriculture.https://www.agronomy.it/index.php/agro/article/view/7 |
| spellingShingle | Elisa Pellegrino Chandra Kamatchi Ramasamy Cristiana Sbrana Paolo Barberi Manuela Giovannetti Selection of Infective Arbuscular Mycorrhizal Fungal Isolates for Field Inoculation Italian Journal of Agronomy |
| title | Selection of Infective Arbuscular Mycorrhizal Fungal Isolates for Field Inoculation |
| title_full | Selection of Infective Arbuscular Mycorrhizal Fungal Isolates for Field Inoculation |
| title_fullStr | Selection of Infective Arbuscular Mycorrhizal Fungal Isolates for Field Inoculation |
| title_full_unstemmed | Selection of Infective Arbuscular Mycorrhizal Fungal Isolates for Field Inoculation |
| title_short | Selection of Infective Arbuscular Mycorrhizal Fungal Isolates for Field Inoculation |
| title_sort | selection of infective arbuscular mycorrhizal fungal isolates for field inoculation |
| url | https://www.agronomy.it/index.php/agro/article/view/7 |
| work_keys_str_mv | AT elisapellegrino selectionofinfectivearbuscularmycorrhizalfungalisolatesforfieldinoculation AT chandrakamatchiramasamy selectionofinfectivearbuscularmycorrhizalfungalisolatesforfieldinoculation AT cristianasbrana selectionofinfectivearbuscularmycorrhizalfungalisolatesforfieldinoculation AT paolobarberi selectionofinfectivearbuscularmycorrhizalfungalisolatesforfieldinoculation AT manuelagiovannetti selectionofinfectivearbuscularmycorrhizalfungalisolatesforfieldinoculation |