Time-resolved fluoroimmunoassay for bactericidal/permeability-increasing protein
Bactericidal/permeability-increasing protein (BPI) is a cationic antimicrobial protein produced by polymorphonuclear leukocytes, that specifically interacts with and kills Gram-negative bacteria. BPl competes with lipopolysaccharide-binding protein (LBP) secreted by liver cells into blood plasma for...
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| Format: | Article |
| Language: | English |
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Wiley
1996-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935196000087 |
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| author | J.-O. Häggblom A. B. Jokilammi-Siltanen H. Peuravuori T. J. Nevalainen |
| author_facet | J.-O. Häggblom A. B. Jokilammi-Siltanen H. Peuravuori T. J. Nevalainen |
| author_sort | J.-O. Häggblom |
| collection | DOAJ |
| description | Bactericidal/permeability-increasing protein (BPI) is a cationic antimicrobial protein produced by polymorphonuclear leukocytes, that specifically interacts with and kills Gram-negative bacteria. BPl competes with lipopolysaccharide-binding protein (LBP) secreted by liver cells into blood plasma for binding to lipopolysaccharide (LPS) and thus reduces the proinflammatory effects of LPS. We have developed a time-resolved fluoroimmunoassay for BPI and measured the concentration of BPI in human serum and plasma samples. The assay is based on a rabbit antibody against recombinant BPI. This antibody specifically adheres to polymorphonuclear leukocytes in immunostained human tissues. The difference in the serum concentration of BPI between unselected hospitalized patients with and without an infection was statistically significant. The mean concentration of BPI in serum samples was 28.3 μg/l (range 1.64–132, S.D. 26.8, n = 83). In contrast, there was no difference between the two groups in the BPI levels in plasma samples. For all individuals tested, BPI levels were consistently higher in plasma samples compared to the matched serum samples. The mean concentration of BPI in plasma samples was 52.3 μg/l (range 0.9–403, S.D. 60.6, n = 90). There was a positive correlation between the concentration of BPI and the white blood cell count as well as between the BPI concentration and C-reactive protein (CRP) in serum samples. In conclusion, the present study demonstrates that BPI can be quantified reliably by time-resolved fluoroimmunoassay in human serum samples. |
| format | Article |
| id | doaj-art-2f9c6ed42a254d5b99afe4106a9847fa |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1996-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-2f9c6ed42a254d5b99afe4106a9847fa2025-08-20T02:08:09ZengWileyMediators of Inflammation0962-93511466-18611996-01-0151475010.1155/S0962935196000087Time-resolved fluoroimmunoassay for bactericidal/permeability-increasing proteinJ.-O. Häggblom0A. B. Jokilammi-Siltanen1H. Peuravuori2T. J. Nevalainen3Department of Pathology, University of Turku, Kiinanmyllynkatu 10, Turku FIN-20520, FinlandDepartment of Pathology, University of Turku, Kiinanmyllynkatu 10, Turku FIN-20520, FinlandDepartment of Pathology, University of Turku, Kiinanmyllynkatu 10, Turku FIN-20520, FinlandDepartment of Pathology, University of Turku, Kiinanmyllynkatu 10, Turku FIN-20520, FinlandBactericidal/permeability-increasing protein (BPI) is a cationic antimicrobial protein produced by polymorphonuclear leukocytes, that specifically interacts with and kills Gram-negative bacteria. BPl competes with lipopolysaccharide-binding protein (LBP) secreted by liver cells into blood plasma for binding to lipopolysaccharide (LPS) and thus reduces the proinflammatory effects of LPS. We have developed a time-resolved fluoroimmunoassay for BPI and measured the concentration of BPI in human serum and plasma samples. The assay is based on a rabbit antibody against recombinant BPI. This antibody specifically adheres to polymorphonuclear leukocytes in immunostained human tissues. The difference in the serum concentration of BPI between unselected hospitalized patients with and without an infection was statistically significant. The mean concentration of BPI in serum samples was 28.3 μg/l (range 1.64–132, S.D. 26.8, n = 83). In contrast, there was no difference between the two groups in the BPI levels in plasma samples. For all individuals tested, BPI levels were consistently higher in plasma samples compared to the matched serum samples. The mean concentration of BPI in plasma samples was 52.3 μg/l (range 0.9–403, S.D. 60.6, n = 90). There was a positive correlation between the concentration of BPI and the white blood cell count as well as between the BPI concentration and C-reactive protein (CRP) in serum samples. In conclusion, the present study demonstrates that BPI can be quantified reliably by time-resolved fluoroimmunoassay in human serum samples.http://dx.doi.org/10.1155/S0962935196000087 |
| spellingShingle | J.-O. Häggblom A. B. Jokilammi-Siltanen H. Peuravuori T. J. Nevalainen Time-resolved fluoroimmunoassay for bactericidal/permeability-increasing protein Mediators of Inflammation |
| title | Time-resolved fluoroimmunoassay for bactericidal/permeability-increasing protein |
| title_full | Time-resolved fluoroimmunoassay for bactericidal/permeability-increasing protein |
| title_fullStr | Time-resolved fluoroimmunoassay for bactericidal/permeability-increasing protein |
| title_full_unstemmed | Time-resolved fluoroimmunoassay for bactericidal/permeability-increasing protein |
| title_short | Time-resolved fluoroimmunoassay for bactericidal/permeability-increasing protein |
| title_sort | time resolved fluoroimmunoassay for bactericidal permeability increasing protein |
| url | http://dx.doi.org/10.1155/S0962935196000087 |
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