Evaluation of Antibiotic Resistance and Biofilm Production among Clinical Strain Isolated from Medical Devices
Microbial biofilms pose a serious threat to patients requiring medical devices (MDs). Prolonged periods of implantation carry a high risk of device-related infections (DRIs). Patients with DRIs often have negative outcomes following the failure of antibiotic treatment. Resistant DRIs are mainly due...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2021/9033278 |
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| author | Veronica Folliero Gianluigi Franci Federica Dell’Annunziata Rosa Giugliano Francesco Foglia Rossella Sperlongano Anna De Filippis Emiliana Finamore Massimiliano Galdiero |
| author_facet | Veronica Folliero Gianluigi Franci Federica Dell’Annunziata Rosa Giugliano Francesco Foglia Rossella Sperlongano Anna De Filippis Emiliana Finamore Massimiliano Galdiero |
| author_sort | Veronica Folliero |
| collection | DOAJ |
| description | Microbial biofilms pose a serious threat to patients requiring medical devices (MDs). Prolonged periods of implantation carry a high risk of device-related infections (DRIs). Patients with DRIs often have negative outcomes following the failure of antibiotic treatment. Resistant DRIs are mainly due to the MDs contamination by bacteria producing biofilm. The present study aimed to detect biofilm formation among MD bacterial isolates and to explore their antibiotic resistance profile. The study was conducted on 76 MDs, collected at University Hospital of Campania “Luigi Vanvitelli,” between October 2019 and September 2020. Identification of isolates and antibiotic susceptibility testing were performed using Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) and Phoenix Becton Dickinson, respectively. Biofilm-forming abilities were assessed using the tissue culture plate (TCP) method. Among the 94 MDs isolated strains, 42.7% were Gram-positive, 40.3% Gram-negative, and 17% Candida species. Among 78 bacterial strains, 43.6% were non-biofilm producers while 56.4% produced biofilms. All biofilm producing isolates were sensitive to a limited spectrum of antibiotic classes. All moderate and strong biofilm producers and 81% of weak biofilm producers were Multidrug Resistance (MDR) strains. In contrast, among non-biofilm producers, only 11.8% were classified as MDR strains. Our results highlighted that Sulfamides and Glycopeptides for the major Gram-positive strains and Fluoroquinolones, Carbapenems, and Aminoglycosides for the most represented Gram-negative isolates could be the most suitable therapeutic choice for most biofilm-DRIs. |
| format | Article |
| id | doaj-art-2f99238e3d3844aabc5ad6699199a550 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-2f99238e3d3844aabc5ad6699199a5502025-02-03T07:24:04ZengWileyInternational Journal of Microbiology1687-918X1687-91982021-01-01202110.1155/2021/90332789033278Evaluation of Antibiotic Resistance and Biofilm Production among Clinical Strain Isolated from Medical DevicesVeronica Folliero0Gianluigi Franci1Federica Dell’Annunziata2Rosa Giugliano3Francesco Foglia4Rossella Sperlongano5Anna De Filippis6Emiliana Finamore7Massimiliano Galdiero8Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples 80138, ItalyDepartment of Medicine Surgery and Dentistry “Scuola Medica Salernitana”, University of Salerno, Fisciano 84081, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples 80138, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples 80138, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples 80138, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples 80138, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples 80138, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples 80138, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples 80138, ItalyMicrobial biofilms pose a serious threat to patients requiring medical devices (MDs). Prolonged periods of implantation carry a high risk of device-related infections (DRIs). Patients with DRIs often have negative outcomes following the failure of antibiotic treatment. Resistant DRIs are mainly due to the MDs contamination by bacteria producing biofilm. The present study aimed to detect biofilm formation among MD bacterial isolates and to explore their antibiotic resistance profile. The study was conducted on 76 MDs, collected at University Hospital of Campania “Luigi Vanvitelli,” between October 2019 and September 2020. Identification of isolates and antibiotic susceptibility testing were performed using Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) and Phoenix Becton Dickinson, respectively. Biofilm-forming abilities were assessed using the tissue culture plate (TCP) method. Among the 94 MDs isolated strains, 42.7% were Gram-positive, 40.3% Gram-negative, and 17% Candida species. Among 78 bacterial strains, 43.6% were non-biofilm producers while 56.4% produced biofilms. All biofilm producing isolates were sensitive to a limited spectrum of antibiotic classes. All moderate and strong biofilm producers and 81% of weak biofilm producers were Multidrug Resistance (MDR) strains. In contrast, among non-biofilm producers, only 11.8% were classified as MDR strains. Our results highlighted that Sulfamides and Glycopeptides for the major Gram-positive strains and Fluoroquinolones, Carbapenems, and Aminoglycosides for the most represented Gram-negative isolates could be the most suitable therapeutic choice for most biofilm-DRIs.http://dx.doi.org/10.1155/2021/9033278 |
| spellingShingle | Veronica Folliero Gianluigi Franci Federica Dell’Annunziata Rosa Giugliano Francesco Foglia Rossella Sperlongano Anna De Filippis Emiliana Finamore Massimiliano Galdiero Evaluation of Antibiotic Resistance and Biofilm Production among Clinical Strain Isolated from Medical Devices International Journal of Microbiology |
| title | Evaluation of Antibiotic Resistance and Biofilm Production among Clinical Strain Isolated from Medical Devices |
| title_full | Evaluation of Antibiotic Resistance and Biofilm Production among Clinical Strain Isolated from Medical Devices |
| title_fullStr | Evaluation of Antibiotic Resistance and Biofilm Production among Clinical Strain Isolated from Medical Devices |
| title_full_unstemmed | Evaluation of Antibiotic Resistance and Biofilm Production among Clinical Strain Isolated from Medical Devices |
| title_short | Evaluation of Antibiotic Resistance and Biofilm Production among Clinical Strain Isolated from Medical Devices |
| title_sort | evaluation of antibiotic resistance and biofilm production among clinical strain isolated from medical devices |
| url | http://dx.doi.org/10.1155/2021/9033278 |
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