Effects of PBAT biodegradable mulch on lettuce (Lactuca sativa L.) physiology and soil microbial community: Based on a long-term degradation trial

Poly (butylene-adipate-co-terephthalate) (PBAT) biodegradable mulch film has been considered a promising, environmentally friendly, and sustainable alternative to polyethylene. However, its long-term effects on crops and soil remain insufficiently understood. This study aimed to investigate the effe...

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Main Authors: Weichang Gao, Zugui Tu, Xie Yin, Shuang Ming, Kai Cai
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Ecotoxicology and Environmental Safety
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Online Access:http://www.sciencedirect.com/science/article/pii/S0147651325010796
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author Weichang Gao
Zugui Tu
Xie Yin
Shuang Ming
Kai Cai
author_facet Weichang Gao
Zugui Tu
Xie Yin
Shuang Ming
Kai Cai
author_sort Weichang Gao
collection DOAJ
description Poly (butylene-adipate-co-terephthalate) (PBAT) biodegradable mulch film has been considered a promising, environmentally friendly, and sustainable alternative to polyethylene. However, its long-term effects on crops and soil remain insufficiently understood. This study aimed to investigate the effects of PBAT mulch film with three application rates (0.05 g/kg, 0.5 g/kg, and 5 g/kg), as well as polyethylene (PE) mulch film (0.05 g/kg), on lettuce (Lactuca sativa L.) growth and soil microbial communities after 4-year pot experiment. The results indicated that the degradation of PBAT biodegradable mulch film in soil was significantly higher than that of PE. The concentrations of chlorophyll a, total chlorophyll, vitamin C, soluble protein, and nitrate in lettuce significantly increased in high application rates of PBAT mulch film. Soil urease (UR) activity increased with the increase of the PBAT application rates, whereas catalase (CAT) activity decreased. Moreover, the application of PBAT biodegradable mulch film significantly altered the composition of soil bacterial and fungal communities. The relative abundances of the bacterial genus Chujaibacter and Pseudolabrys increased with high PBAT application rates. Beneficial fungi such as Amphinema and Trichoderma showed an increasing trend, while pathogenic fungi exhibited the opposite pattern. These trends were also validated with the functional classification of bacteria and fungi. Overall, our findings suggest that PBAT mulch film application exerts significant effects on the soil micro-ecological environment and crop growth.
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institution Kabale University
issn 0147-6513
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publishDate 2025-09-01
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spelling doaj-art-2f89ef4752824dd0b0d450db729fd5c72025-08-20T03:41:53ZengElsevierEcotoxicology and Environmental Safety0147-65132025-09-0130211873410.1016/j.ecoenv.2025.118734Effects of PBAT biodegradable mulch on lettuce (Lactuca sativa L.) physiology and soil microbial community: Based on a long-term degradation trialWeichang Gao0Zugui Tu1Xie Yin2Shuang Ming3Kai Cai4Guizhou Academy of Tobacco Science, Guiyang, Guizhou 550081, China; Corresponding authors.Guizhou Academy of Tobacco Science, Guiyang, Guizhou 550081, China; College of Tobacco Science, Guizhou University, Guiyang, Guizhou 550025, ChinaGuizhou Academy of Tobacco Science, Guiyang, Guizhou 550081, China; College of Tobacco Science, Guizhou University, Guiyang, Guizhou 550025, ChinaGuizhou Academy of Tobacco Science, Guiyang, Guizhou 550081, China; College of Tobacco Science, Guizhou University, Guiyang, Guizhou 550025, ChinaGuizhou Academy of Tobacco Science, Guiyang, Guizhou 550081, China; Corresponding authors.Poly (butylene-adipate-co-terephthalate) (PBAT) biodegradable mulch film has been considered a promising, environmentally friendly, and sustainable alternative to polyethylene. However, its long-term effects on crops and soil remain insufficiently understood. This study aimed to investigate the effects of PBAT mulch film with three application rates (0.05 g/kg, 0.5 g/kg, and 5 g/kg), as well as polyethylene (PE) mulch film (0.05 g/kg), on lettuce (Lactuca sativa L.) growth and soil microbial communities after 4-year pot experiment. The results indicated that the degradation of PBAT biodegradable mulch film in soil was significantly higher than that of PE. The concentrations of chlorophyll a, total chlorophyll, vitamin C, soluble protein, and nitrate in lettuce significantly increased in high application rates of PBAT mulch film. Soil urease (UR) activity increased with the increase of the PBAT application rates, whereas catalase (CAT) activity decreased. Moreover, the application of PBAT biodegradable mulch film significantly altered the composition of soil bacterial and fungal communities. The relative abundances of the bacterial genus Chujaibacter and Pseudolabrys increased with high PBAT application rates. Beneficial fungi such as Amphinema and Trichoderma showed an increasing trend, while pathogenic fungi exhibited the opposite pattern. These trends were also validated with the functional classification of bacteria and fungi. Overall, our findings suggest that PBAT mulch film application exerts significant effects on the soil micro-ecological environment and crop growth.http://www.sciencedirect.com/science/article/pii/S0147651325010796PBAT biodegradable mulch filmLettuce physiologyLong-term degradation trialSoil microbial community
spellingShingle Weichang Gao
Zugui Tu
Xie Yin
Shuang Ming
Kai Cai
Effects of PBAT biodegradable mulch on lettuce (Lactuca sativa L.) physiology and soil microbial community: Based on a long-term degradation trial
Ecotoxicology and Environmental Safety
PBAT biodegradable mulch film
Lettuce physiology
Long-term degradation trial
Soil microbial community
title Effects of PBAT biodegradable mulch on lettuce (Lactuca sativa L.) physiology and soil microbial community: Based on a long-term degradation trial
title_full Effects of PBAT biodegradable mulch on lettuce (Lactuca sativa L.) physiology and soil microbial community: Based on a long-term degradation trial
title_fullStr Effects of PBAT biodegradable mulch on lettuce (Lactuca sativa L.) physiology and soil microbial community: Based on a long-term degradation trial
title_full_unstemmed Effects of PBAT biodegradable mulch on lettuce (Lactuca sativa L.) physiology and soil microbial community: Based on a long-term degradation trial
title_short Effects of PBAT biodegradable mulch on lettuce (Lactuca sativa L.) physiology and soil microbial community: Based on a long-term degradation trial
title_sort effects of pbat biodegradable mulch on lettuce lactuca sativa l physiology and soil microbial community based on a long term degradation trial
topic PBAT biodegradable mulch film
Lettuce physiology
Long-term degradation trial
Soil microbial community
url http://www.sciencedirect.com/science/article/pii/S0147651325010796
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