Metabolic potential of Azotobacter alginate producers and sustainable alternatives for alginate extraction
This research aimed to assess the metabolic activities of Azotobacter vinelandii DT1 strain by assaying its alginate synthesizing enzymes and modifying genes, synthesize alginate from the agricultural residues, and eradicate the pathogenicity and risk associated with Pseudomonas aeruginosa algina...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
ResearchersLinks, Ltd
2024-06-01
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| Series: | Novel Research in Microbiology Journal |
| Subjects: | |
| Online Access: | https://nrmj.journals.ekb.eg/article_361440_66d09f76539aa9922044a65bc2cc9bb2.pdf |
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| Summary: | This research aimed to assess the metabolic activities of Azotobacter vinelandii DT1 strain
by assaying its alginate synthesizing enzymes and modifying genes, synthesize alginate from
the agricultural residues, and eradicate the pathogenicity and risk associated with
Pseudomonas aeruginosa alginate. Preliminary screening for alginate production revealed the
presence of different alginate products. High-performance liquid chromatography (HPLC)
confirmed the existence of varying alginate concentrations, such as sodium alginate
(2.42754×10-1
g/ 100 ml), calcium alginate (1.09597×10-1
g/ 100 ml), acid alginate
(1.39420×10-2
g/ 100 ml), alginate oligosaccharide (8.20576×10-2
g/100 ml), and potassium
alginate (9.78836×10-2
g/ 100 ml). These were accompanied with the corresponding alginate
synthesizing enzymes; mainly GDP-Mannose dehydrogenase (23.77± 0.13 U/ ml);
glycosyltransferase (9.68± 0.53 U/ ml), phosphomannomutase (266.09± 0.16 U/ ml), mannose
phosphate isomerase (95.87± 0.51 U/ ml), alginate lyase (24.50± 0.95 U/ ml), and mannuronan
epimerase (49.93± 0.82 U/ ml). In this study, the expression of alginate-modifying genes such
as alginate lyase and GDP-Mannose dehydrogenase amplicons of Azotobacter vinelandii DT1
strain corresponding to 766 bp, 43 ng and 600 bp, 33 ng molecular weights of the fast DNA
marker; justified the synthesis of different alginate products. Azotobacter alginate synthesis
using a low-cost substrate (i.e., corn cobs) and completely non-pathogenic bacteria
(Azotobacter vinelandii DT1) may be desirable compared to the pathogenicity risks and poor
jellying qualities linked to Pseudomonas alginate biosynthesis, its expensive production costs,
and the adverse environmental effects of seaweed harvesting and processing. |
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| ISSN: | 2537-0286 2537-0294 |