Autonomous microfluidic influenza A/B subtyping system using on-chip multiplex isothermal amplification for field-deployable surveillance

Autonomous microfluidic influenza A/B subtyping system using on-chip multiplex isothermal amplification for field-deployable surveillance

Abstract Influenza viruses present significant challenges to global health. A rapid recombinase polymerase amplification (RPA)-based detection system for multiple influenza strains (A, H1N1/H2N2/H3N2/H5N1/H7N9 and B) has been developed to address the limitations of 2-h Real-Time Quantitative Polymer...

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Bibliographic Details
Main Authors: Si-Ming Lu, Jia-Long Wang, Yang Li, Ling Zhang, Hang-Ping Yao, Li-Guo Liang
Format: Article
Language:English
Published: SpringerOpen 2025-08-01
Series:AMB Express
Subjects:
Microfluidic device
RPA
Influenza A and B subtypes
Point-of-care diagnostics
RNA detection
Low-resource settings
Online Access:https://doi.org/10.1186/s13568-025-01933-2
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Summary:Abstract Influenza viruses present significant challenges to global health. A rapid recombinase polymerase amplification (RPA)-based detection system for multiple influenza strains (A, H1N1/H2N2/H3N2/H5N1/H7N9 and B) has been developed to address the limitations of 2-h Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) tests. By using optimized primers targeting key viral proteins (M, NA, HA, PA), the method achieves detection in 10 min with 0.99 log linearity (1–106 copies/μL). Clinical validation demonstrated 100% sensitivity and specificity, effectively distinguishing infections from healthy controls. This portable platform shows strong potential for point-of-care (POC) applications in resource-limited settings, offering timely diagnosis and epidemic control through its rapid and accurate detection capabilities.
ISSN:2191-0855

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