DYNAMICS OF IL12 CYTOKINE EXPRESSION IN HUMAN MACROPHAGES AFTER TREATMENT WITH DIOXIN
Interleukin IL-12 is a key proinflammatory cytokine, synthesized by macrophages, but the information concerning the dioxin effect on its expression is still fragmentary. The presence of previously identified potential dioxin responsive elements (DREs) in the regulatory regions of IL12A and IL12B gen...
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| Format: | Article |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-01-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/258 |
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| author | D. Y. Oshchepkov E. V. Kashina E. V. Antontseva E. A. Oshchepkova V. A. Mordvinov D. P. Furman |
| author_facet | D. Y. Oshchepkov E. V. Kashina E. V. Antontseva E. A. Oshchepkova V. A. Mordvinov D. P. Furman |
| author_sort | D. Y. Oshchepkov |
| collection | DOAJ |
| description | Interleukin IL-12 is a key proinflammatory cytokine, synthesized by macrophages, but the information concerning the dioxin effect on its expression is still fragmentary. The presence of previously identified potential dioxin responsive elements (DREs) in the regulatory regions of IL12A and IL12B genes, encoding IL-12 subunits IL-12p35 and IL-12p40, respectively, suggests direct activation of these genes by binding of the dioxin/AhR/ARNT complex to DREs. This work proves the binding capacity of these DREs by gel shift assay. The study of the dynamics of IL12A and IL12B gene expression in the human macrophage cell line U937 revealed no influence of dioxin on IL12A expression. In contrast, activation of IL12B gene expression with subsequent inhibition was noted. The observed dynamics can be explained by direct activation of the expression by the dioxin-containing complex and subsequent inhibition of the expression because of oxidative stress caused by dioxin. Thus, the well-known dioxin influence on the immune system can be associated with the difference in the dioxin effect on the expression dynamics of the genes encoding IL-12 subunits. |
| format | Article |
| id | doaj-art-2f8186377aab4adfb6c65b0027253430 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-2f8186377aab4adfb6c65b00272534302025-02-01T09:58:00ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-01-01182354362242DYNAMICS OF IL12 CYTOKINE EXPRESSION IN HUMAN MACROPHAGES AFTER TREATMENT WITH DIOXIND. Y. Oshchepkov0E. V. Kashina1E. V. Antontseva2E. A. Oshchepkova3V. A. Mordvinov4D. P. Furman5Institute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, Russia Novosibirsk State University, Novosibirsk, RussiaInterleukin IL-12 is a key proinflammatory cytokine, synthesized by macrophages, but the information concerning the dioxin effect on its expression is still fragmentary. The presence of previously identified potential dioxin responsive elements (DREs) in the regulatory regions of IL12A and IL12B genes, encoding IL-12 subunits IL-12p35 and IL-12p40, respectively, suggests direct activation of these genes by binding of the dioxin/AhR/ARNT complex to DREs. This work proves the binding capacity of these DREs by gel shift assay. The study of the dynamics of IL12A and IL12B gene expression in the human macrophage cell line U937 revealed no influence of dioxin on IL12A expression. In contrast, activation of IL12B gene expression with subsequent inhibition was noted. The observed dynamics can be explained by direct activation of the expression by the dioxin-containing complex and subsequent inhibition of the expression because of oxidative stress caused by dioxin. Thus, the well-known dioxin influence on the immune system can be associated with the difference in the dioxin effect on the expression dynamics of the genes encoding IL-12 subunits.https://vavilov.elpub.ru/jour/article/view/258macrophagedioxinil-12 |
| spellingShingle | D. Y. Oshchepkov E. V. Kashina E. V. Antontseva E. A. Oshchepkova V. A. Mordvinov D. P. Furman DYNAMICS OF IL12 CYTOKINE EXPRESSION IN HUMAN MACROPHAGES AFTER TREATMENT WITH DIOXIN Вавиловский журнал генетики и селекции macrophage dioxin il-12 |
| title | DYNAMICS OF IL12 CYTOKINE EXPRESSION IN HUMAN MACROPHAGES AFTER TREATMENT WITH DIOXIN |
| title_full | DYNAMICS OF IL12 CYTOKINE EXPRESSION IN HUMAN MACROPHAGES AFTER TREATMENT WITH DIOXIN |
| title_fullStr | DYNAMICS OF IL12 CYTOKINE EXPRESSION IN HUMAN MACROPHAGES AFTER TREATMENT WITH DIOXIN |
| title_full_unstemmed | DYNAMICS OF IL12 CYTOKINE EXPRESSION IN HUMAN MACROPHAGES AFTER TREATMENT WITH DIOXIN |
| title_short | DYNAMICS OF IL12 CYTOKINE EXPRESSION IN HUMAN MACROPHAGES AFTER TREATMENT WITH DIOXIN |
| title_sort | dynamics of il12 cytokine expression in human macrophages after treatment with dioxin |
| topic | macrophage dioxin il-12 |
| url | https://vavilov.elpub.ru/jour/article/view/258 |
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